Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9

The high-affinity K+ transporter HAK5 is the major contributor to root K+ uptake from dilute solutions in K+-starved Arabidopsis plants. Its functionality is tightly regulated and its activity is enhanced under K+ starvation by the transcriptional induction of the AtHAK5 gene, and by the activation...

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Autores: Lara, Alberto, Ródenas, Reyes, Andrés, Zaida, Martínez, Vicente, Quintero, Francisco J., Nieves-Cordones, Manuel, Botella, M. Ángeles, Rubio, Francisco
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Miguel Hernández de Elche
Repositorio:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/32436
Acceso en línea:https://hdl.handle.net/11000/32436
Access Level:acceso abierto
Palabra clave:Arabidopsis
AtCBL
AtCIPK
AtHAK5
potassium
uptake
CDU::5 - Ciencias puras y naturales::57 - Biología
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repository_id_str
spelling Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9Lara, AlbertoRódenas, ReyesAndrés, ZaidaMartínez, VicenteQuintero, Francisco J.Nieves-Cordones, ManuelBotella, M. ÁngelesRubio, FranciscoArabidopsisAtCBLAtCIPKAtHAK5potassiumuptakeCDU::5 - Ciencias puras y naturales::57 - BiologíaThe high-affinity K+ transporter HAK5 is the major contributor to root K+ uptake from dilute solutions in K+-starved Arabidopsis plants. Its functionality is tightly regulated and its activity is enhanced under K+ starvation by the transcriptional induction of the AtHAK5 gene, and by the activation of the transporter via the AtCBL1–AtCIPK23 complex. In the present study, the 26 members of the Arabidopsis CIPK protein kinase family were screened in yeast for their capacity to activate HAK5-mediated K+ uptake. Among them, AtCIPK1 was the most efficient activator of AtHAK5. In addition, AtCIPK9, previously reported to participate in K+ homeostasis, also activated the transporter. In roots, the genes encoding AtCIPK1 and AtCIPK9 were induced by K+ deprivation and atcipk1 and atcipk9 Arabidopsis KO mutants showed a reduced AtHAK5-mediated Rb+ uptake. Activation of AtHAK5 by AtCIPK1 did not occur under hyperosmotic stress conditions, where AtCIPK1 function has been shown to be required to maintain plant growth. Taken together, our data contribute to the identification of the complex regulatory networks that control the highaffinity K+ transporter AtHAK5 and root K+ uptake.Oxford University PressDepartamentos de la UMH::Biología Aplicada202420242020info:eu-repo/semantics/articleapplication/pdf8application/pdfhttps://hdl.handle.net/11000/32436reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheIngléshttps://doi.org/10.1093/jxb/eraa212info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dspace.umh.es:11000/324362026-05-27T13:36:21Z
dc.title.none.fl_str_mv Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9
title Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9
spellingShingle Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9
Lara, Alberto
Arabidopsis
AtCBL
AtCIPK
AtHAK5
potassium
uptake
CDU::5 - Ciencias puras y naturales::57 - Biología
title_short Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9
title_full Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9
title_fullStr Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9
title_full_unstemmed Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9
title_sort Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9
dc.creator.none.fl_str_mv Lara, Alberto
Ródenas, Reyes
Andrés, Zaida
Martínez, Vicente
Quintero, Francisco J.
Nieves-Cordones, Manuel
Botella, M. Ángeles
Rubio, Francisco
author Lara, Alberto
author_facet Lara, Alberto
Ródenas, Reyes
Andrés, Zaida
Martínez, Vicente
Quintero, Francisco J.
Nieves-Cordones, Manuel
Botella, M. Ángeles
Rubio, Francisco
author_role author
author2 Ródenas, Reyes
Andrés, Zaida
Martínez, Vicente
Quintero, Francisco J.
Nieves-Cordones, Manuel
Botella, M. Ángeles
Rubio, Francisco
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Biología Aplicada
dc.subject.none.fl_str_mv Arabidopsis
AtCBL
AtCIPK
AtHAK5
potassium
uptake
CDU::5 - Ciencias puras y naturales::57 - Biología
topic Arabidopsis
AtCBL
AtCIPK
AtHAK5
potassium
uptake
CDU::5 - Ciencias puras y naturales::57 - Biología
description The high-affinity K+ transporter HAK5 is the major contributor to root K+ uptake from dilute solutions in K+-starved Arabidopsis plants. Its functionality is tightly regulated and its activity is enhanced under K+ starvation by the transcriptional induction of the AtHAK5 gene, and by the activation of the transporter via the AtCBL1–AtCIPK23 complex. In the present study, the 26 members of the Arabidopsis CIPK protein kinase family were screened in yeast for their capacity to activate HAK5-mediated K+ uptake. Among them, AtCIPK1 was the most efficient activator of AtHAK5. In addition, AtCIPK9, previously reported to participate in K+ homeostasis, also activated the transporter. In roots, the genes encoding AtCIPK1 and AtCIPK9 were induced by K+ deprivation and atcipk1 and atcipk9 Arabidopsis KO mutants showed a reduced AtHAK5-mediated Rb+ uptake. Activation of AtHAK5 by AtCIPK1 did not occur under hyperosmotic stress conditions, where AtCIPK1 function has been shown to be required to maintain plant growth. Taken together, our data contribute to the identification of the complex regulatory networks that control the highaffinity K+ transporter AtHAK5 and root K+ uptake.
publishDate 2020
dc.date.none.fl_str_mv 2020
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/32436
url https://hdl.handle.net/11000/32436
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1093/jxb/eraa212
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
8
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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