PACIFIC: a lightweight deep-learning classifier of SARS-CoV-2 and co-infecting RNA viruses

Viral co-infections occur in COVID-19 patients, potentially impacting disease progression and severity. However, there is currently no dedicated method to identify viral co-infections in patient RNA-seq data. We developed PACIFIC, a deep-learning algorithm that accurately detects SARS-CoV-2 and othe...

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Detalles Bibliográficos
Autores: Acera Mateos, Pablo, Balboa, Renzo F., Easteal, Simon, Eyras Jiménez, Eduardo, Patel, Hardip R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/48162
Acceso en línea:http://hdl.handle.net/10230/48162
http://dx.doi.org/10.1038/s41598-021-82043-4
Access Level:acceso abierto
Palabra clave:Clinical microbiology
Computational biology and bioinformatics
Genetics
Infectious-disease diagnostics
Microbiology
Molecular biology
Pathogens
Virology
Descripción
Sumario:Viral co-infections occur in COVID-19 patients, potentially impacting disease progression and severity. However, there is currently no dedicated method to identify viral co-infections in patient RNA-seq data. We developed PACIFIC, a deep-learning algorithm that accurately detects SARS-CoV-2 and other common RNA respiratory viruses from RNA-seq data. Using in silico data, PACIFIC recovers the presence and relative concentrations of viruses with > 99% precision and recall. PACIFIC accurately detects SARS-CoV-2 and other viral infections in 63 independent in vitro cell culture and patient datasets. PACIFIC is an end-to-end tool that enables the systematic monitoring of viral infections in the current global pandemic.