Caveats on the use of rotenone to estimate mixotrophic grazing in the oceans

11 pages, 5 figures, 2 tables

Detalles Bibliográficos
Autores: Duarte Ferreira, Guilherme, Calbet, Albert
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/206447
Acceso en línea:http://hdl.handle.net/10261/206447
Access Level:acceso abierto
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spelling Caveats on the use of rotenone to estimate mixotrophic grazing in the oceansDuarte Ferreira, GuilhermeCalbet, Albert11 pages, 5 figures, 2 tablesPhagotrophic mixotrophs (mixoplankton) are now widely recognised as important members of food webs, but their role in the functioning of food webs is not yet fully understood. This is due to the lack of a well-established technique to estimate mixotrophic grazing. An immediate step in this direction would be the development of a method that separates mixotrophic from heterotrophic grazing that can be routinely incorporated into the common techniques used to measure microplankton herbivory (e.g., the dilution technique). This idea was explored by the addition of rotenone, an inhibitor of the respiratory electron chain that has been widely used to selectively eliminate metazoans, both in the field and in the laboratory. Accordingly, rotenone was added to auto-, mixo-, and heterotrophic protist cultures in increasing concentrations (ca. 24 h). The results showed that mixotrophs survived better than heterotrophs at low concentrations of rotenone. Nevertheless, their predation was more affected, rendering rotenone unusable as a heterotrophic grazing deterrent. Additionally, it was found that rotenone had a differential effect depending on the growth phase of an autotrophic culture. Altogether, these results suggest that previous uses of rotenone in the field may have disrupted the planktonic food webThis project is a contribution of the Marine Zooplankton Ecology Group from the Generalitat de Catalunya (2017 SGR 87) and has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 766327With the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI)Springer NatureEuropean CommissionGeneralitat de CatalunyaAgencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/206447reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/766327https://doi.org/10.1038/s41598-020-60764-2Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2064472026-05-22T06:33:51Z
dc.title.none.fl_str_mv Caveats on the use of rotenone to estimate mixotrophic grazing in the oceans
title Caveats on the use of rotenone to estimate mixotrophic grazing in the oceans
spellingShingle Caveats on the use of rotenone to estimate mixotrophic grazing in the oceans
Duarte Ferreira, Guilherme
title_short Caveats on the use of rotenone to estimate mixotrophic grazing in the oceans
title_full Caveats on the use of rotenone to estimate mixotrophic grazing in the oceans
title_fullStr Caveats on the use of rotenone to estimate mixotrophic grazing in the oceans
title_full_unstemmed Caveats on the use of rotenone to estimate mixotrophic grazing in the oceans
title_sort Caveats on the use of rotenone to estimate mixotrophic grazing in the oceans
dc.creator.none.fl_str_mv Duarte Ferreira, Guilherme
Calbet, Albert
author Duarte Ferreira, Guilherme
author_facet Duarte Ferreira, Guilherme
Calbet, Albert
author_role author
author2 Calbet, Albert
author2_role author
dc.contributor.none.fl_str_mv European Commission
Generalitat de Catalunya
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description 11 pages, 5 figures, 2 tables
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/206447
url http://hdl.handle.net/10261/206447
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/766327
https://doi.org/10.1038/s41598-020-60764-2
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
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instname:Consejo Superior de Investigaciones Científicas (CSIC)
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