Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis

Cholangiocarcinoma (CCA) comprises a group of heterogeneous biliary cancers with dismal prognosis. The etiologies of most CCAs are unknown, but primary sclerosing cholangitis (PSC) is a risk factor. Non-invasive diagnosis of CCA is challenging and accurate biomarkers are lacking. We aimed to charact...

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Detalles Bibliográficos
Autores: Lapitz Dambolenea, Ainhoa, Arbelaiz Cossio, Ander, O'Rourke, Colm J., Lavín, José L., La Casta, Adelaida, Ibarra, César, Jimeno, Juan P., Santos Laso, Álvaro, Izquierdo Sánchez, Laura, Krawczyk, Marcin, Perugorria Montiel, María Jesús, Jiménez Agüero, Raúl, Sánchez Campos, Alberto, Riaño Fernández, Ioana, González, Esperanza, Lammert, Frank, Marzioni, Marco, Rodríguez Macías, Rocío Isabel, García Marín, Jose Juan, Karlsen, Tom H., Bujanda Fernández de Pierola, Luis, Falcón Pérez, Juan Manuel, Andersen, Jesper B., Aransay Bañares, Ana María, Rodrigues, Pedro M., Bañales Asurmendi, Jesús María
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/42599
Acceso en línea:http://hdl.handle.net/10810/42599
Access Level:acceso abierto
Palabra clave:biomarkers
cholangiocarcinoma
extracellular vesicles
liquid biopsy
transcriptomics
Descripción
Sumario:Cholangiocarcinoma (CCA) comprises a group of heterogeneous biliary cancers with dismal prognosis. The etiologies of most CCAs are unknown, but primary sclerosing cholangitis (PSC) is a risk factor. Non-invasive diagnosis of CCA is challenging and accurate biomarkers are lacking. We aimed to characterize the transcriptomic profile of serum and urine extracellular vesicles (EVs) from patients with CCA, PSC, ulcerative colitis (UC), and healthy individuals. Serum and urine EVs were isolated by serial ultracentrifugations and characterized by nanoparticle tracking analysis, transmission electron microscopy, and immunoblotting. EVs transcriptome was determined by Illumina gene expression array [messenger RNAs (mRNA) and non-coding RNAs (ncRNAs)]. Differential RNA profiles were found in serum and urine EVs from patients with CCA compared to control groups (disease and healthy), showing high diagnostic capacity. The comparison of the mRNA profiles of serum or urine EVs from patients with CCA with the transcriptome of tumor tissues from two cohorts of patients, CCA cells in vitro, and CCA cells-derived EVs, identified 105 and 39 commonly-altered transcripts, respectively. Gene ontology analysis indicated that most commonly-altered mRNAs participate in carcinogenic steps. Overall, patients with CCA present specific RNA profiles in EVs mirroring the tumor, and constituting novel promising liquid biopsy biomarkers.

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