Cloning and functional characterization of an elovl4-like gene involved in the biosynthesis of long-chain polyunsaturated fatty acids in the swimming crab Portunus trituberculatus

Elongation of very long-chain fatty acid 4 (Elovl4) proteins participate in the biosynthesis of long-chain and very long-chain polyunsaturated fatty acids (LC-PUFA and VLC-PUFA). In the present study, an elovl4 cDNA was cloned from the swimming crab Portunus trituberculatus by PCR techniques and fun...

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Detalles Bibliográficos
Autores: Sun, Peng, Zhou, Qicun, Monroig, Óscar, Navarro, Juan Carlos, Jin, Min, Yuan, Ye, Wang, Xuexi, Jiao, Lefei
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/202165
Acceso en línea:http://hdl.handle.net/10261/202165
Access Level:acceso abierto
Palabra clave:Elovl4-like
LC-PUFA
Functional characterization
Portunus trituberculatus
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spelling Cloning and functional characterization of an elovl4-like gene involved in the biosynthesis of long-chain polyunsaturated fatty acids in the swimming crab Portunus trituberculatusSun, PengZhou, QicunMonroig, ÓscarNavarro, Juan CarlosJin, MinYuan, YeWang, XuexiJiao, LefeiElovl4-likeLC-PUFAFunctional characterizationPortunus trituberculatusElongation of very long-chain fatty acid 4 (Elovl4) proteins participate in the biosynthesis of long-chain and very long-chain polyunsaturated fatty acids (LC-PUFA and VLC-PUFA). In the present study, an elovl4 cDNA was cloned from the swimming crab Portunus trituberculatus by PCR techniques and functionally characterized using recombinant expression in yeast Saccharomyces cerevisiae. The elovl4 cDNA sequence contained an open reading frame of 1038 base pairs, encoding a protein of 346 amino acids. The elovl4 has typical Elovl structures, with transmembrane domains (6) and a histidine box. The elovl4 was expressed in various tissues analyzed, with the highest expression found in intestine and hepatopancreas, followed by stomach and eyestalk. The functional characterization of Elovl4 yeast showed that the P. trituberculatus Elovl4 can elongate C18–22 polyunsaturated fatty acids (PUFA), reaching in some cases products of C24 and C26. Along its ability to elongate PUFA, the P. trituberculatus Elovl4 was also efficient in the elongation of saturated fatty acids, with 28:0 and 30:0 being prominent elongation products. These results provide insight into the LC-PUFA biosynthetic capability of commercially important species of crustaceans.This study was supported by National Key R & D Program of China (2018YFD0900400), China Agriculture Research System-48 (CARS-48), the National Natural Science Foundation of China (41476125), and the Nature Science Foundation of Zhejiang Province (LY17C190002), Key Research Program of Zhejiang Province of China (2018C02037), the Major Agriculture Program of Ningbo (2017C110007). This research was also sponsored by the K. C. Wong Magna Fund in Ningbo University. Further funding was obtained through a Proyecto Intramural Especial of CSIC (201840I016) awarded to ÓM.Peer reviewedElsevierNational Key Research and Development Program (China)National Natural Science Foundation of ChinaAgriculture Research System of ChinaZhejiang Provincial Natural Science FoundationK. C. Wong Magna FundNavarro, Juan Carlos [0000-0001-6976-6686]Monroig, Óscar [0000-0001-8712-0440]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/202165reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.cbpb.2020.110408Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2021652026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cloning and functional characterization of an elovl4-like gene involved in the biosynthesis of long-chain polyunsaturated fatty acids in the swimming crab Portunus trituberculatus
title Cloning and functional characterization of an elovl4-like gene involved in the biosynthesis of long-chain polyunsaturated fatty acids in the swimming crab Portunus trituberculatus
spellingShingle Cloning and functional characterization of an elovl4-like gene involved in the biosynthesis of long-chain polyunsaturated fatty acids in the swimming crab Portunus trituberculatus
Sun, Peng
Elovl4-like
LC-PUFA
Functional characterization
Portunus trituberculatus
title_short Cloning and functional characterization of an elovl4-like gene involved in the biosynthesis of long-chain polyunsaturated fatty acids in the swimming crab Portunus trituberculatus
title_full Cloning and functional characterization of an elovl4-like gene involved in the biosynthesis of long-chain polyunsaturated fatty acids in the swimming crab Portunus trituberculatus
title_fullStr Cloning and functional characterization of an elovl4-like gene involved in the biosynthesis of long-chain polyunsaturated fatty acids in the swimming crab Portunus trituberculatus
title_full_unstemmed Cloning and functional characterization of an elovl4-like gene involved in the biosynthesis of long-chain polyunsaturated fatty acids in the swimming crab Portunus trituberculatus
title_sort Cloning and functional characterization of an elovl4-like gene involved in the biosynthesis of long-chain polyunsaturated fatty acids in the swimming crab Portunus trituberculatus
dc.creator.none.fl_str_mv Sun, Peng
Zhou, Qicun
Monroig, Óscar
Navarro, Juan Carlos
Jin, Min
Yuan, Ye
Wang, Xuexi
Jiao, Lefei
author Sun, Peng
author_facet Sun, Peng
Zhou, Qicun
Monroig, Óscar
Navarro, Juan Carlos
Jin, Min
Yuan, Ye
Wang, Xuexi
Jiao, Lefei
author_role author
author2 Zhou, Qicun
Monroig, Óscar
Navarro, Juan Carlos
Jin, Min
Yuan, Ye
Wang, Xuexi
Jiao, Lefei
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv National Key Research and Development Program (China)
National Natural Science Foundation of China
Agriculture Research System of China
Zhejiang Provincial Natural Science Foundation
K. C. Wong Magna Fund
Navarro, Juan Carlos [0000-0001-6976-6686]
Monroig, Óscar [0000-0001-8712-0440]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Elovl4-like
LC-PUFA
Functional characterization
Portunus trituberculatus
topic Elovl4-like
LC-PUFA
Functional characterization
Portunus trituberculatus
description Elongation of very long-chain fatty acid 4 (Elovl4) proteins participate in the biosynthesis of long-chain and very long-chain polyunsaturated fatty acids (LC-PUFA and VLC-PUFA). In the present study, an elovl4 cDNA was cloned from the swimming crab Portunus trituberculatus by PCR techniques and functionally characterized using recombinant expression in yeast Saccharomyces cerevisiae. The elovl4 cDNA sequence contained an open reading frame of 1038 base pairs, encoding a protein of 346 amino acids. The elovl4 has typical Elovl structures, with transmembrane domains (6) and a histidine box. The elovl4 was expressed in various tissues analyzed, with the highest expression found in intestine and hepatopancreas, followed by stomach and eyestalk. The functional characterization of Elovl4 yeast showed that the P. trituberculatus Elovl4 can elongate C18–22 polyunsaturated fatty acids (PUFA), reaching in some cases products of C24 and C26. Along its ability to elongate PUFA, the P. trituberculatus Elovl4 was also efficient in the elongation of saturated fatty acids, with 28:0 and 30:0 being prominent elongation products. These results provide insight into the LC-PUFA biosynthetic capability of commercially important species of crustaceans.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/202165
url http://hdl.handle.net/10261/202165
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.cbpb.2020.110408
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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