A multifunctionalized potyvirus-derived nanoparticle that targets and internalizes into cancer cells

Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen...

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Autores: Truchado Martín, Daniel Alejandro, Juárez-Molina, María, Rincón, Sara, Zurita, Lucía, Tomé-Amat, Jaime, Lorz López, María Corina, Ponz, Fernando
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/103238
Acceso en línea:https://hdl.handle.net/20.500.14352/103238
Access Level:acceso abierto
Palabra clave:578
577.2
616-006.04
Turnip mosaic virus
VLP
Protein A
Z domain
Cetuximab
Squamous cell carcinoma
Viral nanoparticles
Microbiología (Biología)
Biotecnología
Oncología
3201.01 Oncología
2414 Microbiología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/103238
network_acronym_str ES
network_name_str España
repository_id_str
spelling A multifunctionalized potyvirus-derived nanoparticle that targets and internalizes into cancer cellsTruchado Martín, Daniel AlejandroJuárez-Molina, MaríaRincón, SaraZurita, LucíaTomé-Amat, JaimeLorz López, María CorinaPonz, Fernando578577.2616-006.04Turnip mosaic virusVLPProtein AZ domainCetuximabSquamous cell carcinomaViral nanoparticlesMicrobiología (Biología)BiotecnologíaOncología3201.01 Oncología2414 MicrobiologíaPlant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen, that exhibits a high affinity for immunoglobulins G (IgG) thanks to its functionalization with the Z domain of staphylococcal Protein A via gene fusion. We selected cetuximab as a model IgG to demonstrate the versatility of this novel TuMV VNP by developing a fluorescent nanoplatform to mark tumoral cells from the Cal33 line of a tongue squamous cell carcinoma. Using confocal microscopy, we observed that fluorescent VNP–cetuximab bound selectively to Cal33 and was internalized, revealing the potential of this nanotool in cancer research.MDPIUniversidad Complutense de Madrid20242024-04-1320242024-04-13journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/103238reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1032382026-06-02T12:44:21Z
dc.title.none.fl_str_mv A multifunctionalized potyvirus-derived nanoparticle that targets and internalizes into cancer cells
title A multifunctionalized potyvirus-derived nanoparticle that targets and internalizes into cancer cells
spellingShingle A multifunctionalized potyvirus-derived nanoparticle that targets and internalizes into cancer cells
Truchado Martín, Daniel Alejandro
578
577.2
616-006.04
Turnip mosaic virus
VLP
Protein A
Z domain
Cetuximab
Squamous cell carcinoma
Viral nanoparticles
Microbiología (Biología)
Biotecnología
Oncología
3201.01 Oncología
2414 Microbiología
title_short A multifunctionalized potyvirus-derived nanoparticle that targets and internalizes into cancer cells
title_full A multifunctionalized potyvirus-derived nanoparticle that targets and internalizes into cancer cells
title_fullStr A multifunctionalized potyvirus-derived nanoparticle that targets and internalizes into cancer cells
title_full_unstemmed A multifunctionalized potyvirus-derived nanoparticle that targets and internalizes into cancer cells
title_sort A multifunctionalized potyvirus-derived nanoparticle that targets and internalizes into cancer cells
dc.creator.none.fl_str_mv Truchado Martín, Daniel Alejandro
Juárez-Molina, María
Rincón, Sara
Zurita, Lucía
Tomé-Amat, Jaime
Lorz López, María Corina
Ponz, Fernando
author Truchado Martín, Daniel Alejandro
author_facet Truchado Martín, Daniel Alejandro
Juárez-Molina, María
Rincón, Sara
Zurita, Lucía
Tomé-Amat, Jaime
Lorz López, María Corina
Ponz, Fernando
author_role author
author2 Juárez-Molina, María
Rincón, Sara
Zurita, Lucía
Tomé-Amat, Jaime
Lorz López, María Corina
Ponz, Fernando
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 578
577.2
616-006.04
Turnip mosaic virus
VLP
Protein A
Z domain
Cetuximab
Squamous cell carcinoma
Viral nanoparticles
Microbiología (Biología)
Biotecnología
Oncología
3201.01 Oncología
2414 Microbiología
topic 578
577.2
616-006.04
Turnip mosaic virus
VLP
Protein A
Z domain
Cetuximab
Squamous cell carcinoma
Viral nanoparticles
Microbiología (Biología)
Biotecnología
Oncología
3201.01 Oncología
2414 Microbiología
description Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen, that exhibits a high affinity for immunoglobulins G (IgG) thanks to its functionalization with the Z domain of staphylococcal Protein A via gene fusion. We selected cetuximab as a model IgG to demonstrate the versatility of this novel TuMV VNP by developing a fluorescent nanoplatform to mark tumoral cells from the Cal33 line of a tongue squamous cell carcinoma. Using confocal microscopy, we observed that fluorescent VNP–cetuximab bound selectively to Cal33 and was internalized, revealing the potential of this nanotool in cancer research.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-04-13
2024
2024-04-13
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/103238
url https://hdl.handle.net/20.500.14352/103238
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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