Toxic effects of the cylindrospermopsin and chlorpyrifos combination on the differentiated SH-SY5Y human neuroblastoma cell line

Due to climate change and anthropogenic activities, the levels of pollution of aquatic and terrestrial environments have increased in the last decades. In this sense, the rise of cyanobacterial blooms, which release secondary metabolites with toxic properties, and the global use of pesticides for ag...

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Autores: Hinojosa, María G., Gutiérrez-Praena, Daniel, López, Sergio, Prieto Márquez, Ana Isabel, Moreno, Francisco J., Jos, Ángeles, Cameán Fernández, A. M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/340132
Acceso en línea:http://hdl.handle.net/10261/340132
https://api.elsevier.com/content/abstract/scopus_id/85150870207
Access Level:acceso abierto
Palabra clave:Acetylcholinesterase activity
Chlorpyrifos
Cylindrospermopsin
Cytotoxicity
Emerging contaminants
SH-SY5Y cells
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spelling Toxic effects of the cylindrospermopsin and chlorpyrifos combination on the differentiated SH-SY5Y human neuroblastoma cell lineHinojosa, María G.Gutiérrez-Praena, DanielLópez, SergioPrieto Márquez, Ana IsabelMoreno, Francisco J.Jos, ÁngelesCameán Fernández, A. M.Acetylcholinesterase activityChlorpyrifosCylindrospermopsinCytotoxicityEmerging contaminantsSH-SY5Y cellsDue to climate change and anthropogenic activities, the levels of pollution of aquatic and terrestrial environments have increased in the last decades. In this sense, the rise of cyanobacterial blooms, which release secondary metabolites with toxic properties, and the global use of pesticides for agricultural purposes have a negative impact on ecosystems. Thus, it would be interesting to study the concomitance of both types of toxicants in the same sample, since it is possible that they appear together. The aim of the present work was to state the effects of the interaction between the cyanotoxin cylindrospermopsin and the pesticide chlorpyrifos in differentiated SH-SY5Y neuronal cells to assess how they could affect the nervous system. To this end, cytotoxicity, morphological, and acetylcholinesterase activity studies were performed during 24 and 48 h. The results revealed a concentration-dependent decrease in viability and interaction between both toxicants, together with clear signs of apoptosis and necrosis induction. In this sense, different stages on the differentiation process would lead to differences in the toxicity exerted by the compounds both isolated as in combination, which it is not observed in non-differentiated cells. Additionally, the acetylcholinesterase activity appeared not to be affected, which is a clear difference compared to non-differentiated cells. These results show the importance of studying not only the toxicants themselves, but also in combination, to assess their possible effects in a more realistic scenario.The authors want to thank to the Spanish Ministerio de Economía y Competitividad [AGL 2015-64558-R, MINECO/FEDER, Spain, UE], the Spanish Ministerio de Ciencia e Innovación [PID 2019-104890RB-I00, MICINN, Spain] for their financial support. Sergio López also wants to thank to the “V Plan Propio” of the Universidad de Sevilla [VPPI-US, Sevilla, Spain] for his contract (cofounded by the European Social Fund).Peer reviewedElsevierMinisterio de Economía y Competitividad (España)European CommissionMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Universidad de SevillaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/340132https://api.elsevier.com/content/abstract/scopus_id/85150870207reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2015-64558-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104890RB-I00https://doi.org/10.1016/j.toxicon.2023.107091Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3401322026-05-22T06:33:51Z
dc.title.none.fl_str_mv Toxic effects of the cylindrospermopsin and chlorpyrifos combination on the differentiated SH-SY5Y human neuroblastoma cell line
title Toxic effects of the cylindrospermopsin and chlorpyrifos combination on the differentiated SH-SY5Y human neuroblastoma cell line
spellingShingle Toxic effects of the cylindrospermopsin and chlorpyrifos combination on the differentiated SH-SY5Y human neuroblastoma cell line
Hinojosa, María G.
Acetylcholinesterase activity
Chlorpyrifos
Cylindrospermopsin
Cytotoxicity
Emerging contaminants
SH-SY5Y cells
title_short Toxic effects of the cylindrospermopsin and chlorpyrifos combination on the differentiated SH-SY5Y human neuroblastoma cell line
title_full Toxic effects of the cylindrospermopsin and chlorpyrifos combination on the differentiated SH-SY5Y human neuroblastoma cell line
title_fullStr Toxic effects of the cylindrospermopsin and chlorpyrifos combination on the differentiated SH-SY5Y human neuroblastoma cell line
title_full_unstemmed Toxic effects of the cylindrospermopsin and chlorpyrifos combination on the differentiated SH-SY5Y human neuroblastoma cell line
title_sort Toxic effects of the cylindrospermopsin and chlorpyrifos combination on the differentiated SH-SY5Y human neuroblastoma cell line
dc.creator.none.fl_str_mv Hinojosa, María G.
Gutiérrez-Praena, Daniel
López, Sergio
Prieto Márquez, Ana Isabel
Moreno, Francisco J.
Jos, Ángeles
Cameán Fernández, A. M.
author Hinojosa, María G.
author_facet Hinojosa, María G.
Gutiérrez-Praena, Daniel
López, Sergio
Prieto Márquez, Ana Isabel
Moreno, Francisco J.
Jos, Ángeles
Cameán Fernández, A. M.
author_role author
author2 Gutiérrez-Praena, Daniel
López, Sergio
Prieto Márquez, Ana Isabel
Moreno, Francisco J.
Jos, Ángeles
Cameán Fernández, A. M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Universidad de Sevilla
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Acetylcholinesterase activity
Chlorpyrifos
Cylindrospermopsin
Cytotoxicity
Emerging contaminants
SH-SY5Y cells
topic Acetylcholinesterase activity
Chlorpyrifos
Cylindrospermopsin
Cytotoxicity
Emerging contaminants
SH-SY5Y cells
description Due to climate change and anthropogenic activities, the levels of pollution of aquatic and terrestrial environments have increased in the last decades. In this sense, the rise of cyanobacterial blooms, which release secondary metabolites with toxic properties, and the global use of pesticides for agricultural purposes have a negative impact on ecosystems. Thus, it would be interesting to study the concomitance of both types of toxicants in the same sample, since it is possible that they appear together. The aim of the present work was to state the effects of the interaction between the cyanotoxin cylindrospermopsin and the pesticide chlorpyrifos in differentiated SH-SY5Y neuronal cells to assess how they could affect the nervous system. To this end, cytotoxicity, morphological, and acetylcholinesterase activity studies were performed during 24 and 48 h. The results revealed a concentration-dependent decrease in viability and interaction between both toxicants, together with clear signs of apoptosis and necrosis induction. In this sense, different stages on the differentiation process would lead to differences in the toxicity exerted by the compounds both isolated as in combination, which it is not observed in non-differentiated cells. Additionally, the acetylcholinesterase activity appeared not to be affected, which is a clear difference compared to non-differentiated cells. These results show the importance of studying not only the toxicants themselves, but also in combination, to assess their possible effects in a more realistic scenario.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/340132
https://api.elsevier.com/content/abstract/scopus_id/85150870207
url http://hdl.handle.net/10261/340132
https://api.elsevier.com/content/abstract/scopus_id/85150870207
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//AGL2015-64558-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104890RB-I00
https://doi.org/10.1016/j.toxicon.2023.107091
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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