Multi-Omics Methods Applied to Flower Development

Developmental processes in multicellular organisms depend on the proficiency of cells to orchestrate different gene expression programs. Over the past years, several studies of reproductive organ development have considered genomic analyses of transcription factors and global gene expression changes...

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Detalles Bibliográficos
Autores: Álvarez Urdiola, Raquel, Matus, José Tomás|||0000-0002-9196-1813, Riechmann, José Luis|||0000-0003-1108-1945
Tipo de recurso: capítulo de libro
Fecha de publicación:2023
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:312864
Acceso en línea:https://ddd.uab.cat/record/312864
https://dx.doi.org/urn:doi:10.1007/978-1-0716-3299-4_23
Access Level:acceso abierto
Palabra clave:Protein extraction
RNA extraction
Proteomics
Transcriptomics
Flower development
LC-MS/MS
Arabidopsis
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spelling Multi-Omics Methods Applied to Flower DevelopmentÁlvarez Urdiola, RaquelMatus, José Tomás|||0000-0002-9196-1813Riechmann, José Luis|||0000-0003-1108-1945Protein extractionRNA extractionProteomicsTranscriptomicsFlower developmentLC-MS/MSArabidopsisDevelopmental processes in multicellular organisms depend on the proficiency of cells to orchestrate different gene expression programs. Over the past years, several studies of reproductive organ development have considered genomic analyses of transcription factors and global gene expression changes, modeling complex gene regulatory networks. Nevertheless, the dynamic view of developmental processes requires, as well, the study of the proteome in its expression, complexity, and relationship with the transcriptome. In this chapter, we describe a dual extraction method-for protein and RNA-for the characterization of genome expression at proteome level and its correlation to transcript expression data. We also present a shotgun proteomic procedure (LC-MS/MS) followed by a pipeline for the imputation of missing values in mass spectrometry results.Humana 22023-01-0120232023-01-01Capítol de llibrehttp://purl.org/coar/resource_type/c_3248AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/bookPartapplication/pdfhttps://ddd.uab.cat/record/312864https://dx.doi.org/urn:doi:10.1007/978-1-0716-3299-4_23reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 BFU2014-58289-PAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-718Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2015-0533Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PRE2018-084278open accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3128642026-06-06T12:50:31Z
dc.title.none.fl_str_mv Multi-Omics Methods Applied to Flower Development
title Multi-Omics Methods Applied to Flower Development
spellingShingle Multi-Omics Methods Applied to Flower Development
Álvarez Urdiola, Raquel
Protein extraction
RNA extraction
Proteomics
Transcriptomics
Flower development
LC-MS/MS
Arabidopsis
title_short Multi-Omics Methods Applied to Flower Development
title_full Multi-Omics Methods Applied to Flower Development
title_fullStr Multi-Omics Methods Applied to Flower Development
title_full_unstemmed Multi-Omics Methods Applied to Flower Development
title_sort Multi-Omics Methods Applied to Flower Development
dc.creator.none.fl_str_mv Álvarez Urdiola, Raquel
Matus, José Tomás|||0000-0002-9196-1813
Riechmann, José Luis|||0000-0003-1108-1945
author Álvarez Urdiola, Raquel
author_facet Álvarez Urdiola, Raquel
Matus, José Tomás|||0000-0002-9196-1813
Riechmann, José Luis|||0000-0003-1108-1945
author_role author
author2 Matus, José Tomás|||0000-0002-9196-1813
Riechmann, José Luis|||0000-0003-1108-1945
author2_role author
author
dc.subject.none.fl_str_mv Protein extraction
RNA extraction
Proteomics
Transcriptomics
Flower development
LC-MS/MS
Arabidopsis
topic Protein extraction
RNA extraction
Proteomics
Transcriptomics
Flower development
LC-MS/MS
Arabidopsis
description Developmental processes in multicellular organisms depend on the proficiency of cells to orchestrate different gene expression programs. Over the past years, several studies of reproductive organ development have considered genomic analyses of transcription factors and global gene expression changes, modeling complex gene regulatory networks. Nevertheless, the dynamic view of developmental processes requires, as well, the study of the proteome in its expression, complexity, and relationship with the transcriptome. In this chapter, we describe a dual extraction method-for protein and RNA-for the characterization of genome expression at proteome level and its correlation to transcript expression data. We also present a shotgun proteomic procedure (LC-MS/MS) followed by a pipeline for the imputation of missing values in mass spectrometry results.
publishDate 2023
dc.date.none.fl_str_mv 2
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv Capítol de llibre
http://purl.org/coar/resource_type/c_3248
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/312864
https://dx.doi.org/urn:doi:10.1007/978-1-0716-3299-4_23
url https://ddd.uab.cat/record/312864
https://dx.doi.org/urn:doi:10.1007/978-1-0716-3299-4_23
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BFU2014-58289-P
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-718
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2015-0533
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PRE2018-084278
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Humana
publisher.none.fl_str_mv Humana
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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