Increased power by harmonizing structural MRI site differences with the comBat batch adjustment method in ENIGMA

A common limitation of neuroimaging studies is their small sample sizes. To overcome this hurdle, the Enhancing Neuro Imaging Genetics through Meta-Analysis (ENIGMA) Consortium combines neuroimaging data from many institutions worldwide. However, this introduces heterogeneity due to different scanni...

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Autores: Radua, Joaquim, Vieta i Pascual, Eduard, 1963-, Shinohara, Russell, Kochunov, Peter, Quidé, Yann, Green, Melissa J., Weickert, Cynthia S., Weickert, Thomas, Bruggemann, Jason, Kircher, Tilo, Nenadic, Igor, Cairns, Murray J., Seal, Marc, Schall, Ulrich, Henskens, Frans, Fullerton, Janice M., Mowry, Bryan, Pantelis, Christos, Lenroot, Rhoshel, Cropley, Vanessa, Loughland, Carmel, Scott, Rodney J., Wolf, Daniel, Satterthwaite, Theodore D., Tan, Yunlong, Sim, Kang, Piras, Fabrizio, Spalletta, Gianfranco, Banaj, Nerisa, Pomarol-Clotet, Edith, Solanes, Aleix, Albajes Eizagirre, Anton, Canales Rodríguez, Erick Jorge, Sarró, Salvador, Di Giorgio, Annabella, Bertolino, Alessandro, Stäblein, Michael, Oertel, Viola, Knöchel, Christian, Borgwardt, Stefan, du Plessis, Stefan, Yun, Je-Yeon, Kwon, Jun Soo, Dannlowski, Udo, Hahn, Tim, Grotegerd, Dominik, Alloza, Clara, Arango López, Celso, Janssen, Joost, Díaz Caneja, Covadonga M., Jiang, Wenhao, Calhoun, Vince, Ehrlich, Stefan, Yang, Kun, Cascella, Nicola G., Takayanagi, Yoichiro, Sawa, Akira, Tomyshev, Alexander, Lebedeva, Irina, Kaleda, Vasily, Kirschner, Matthias, Hoschl, Cyril, Tomecek, David, Skoch, Antonin, van Amelsvoort, Therese, Bakker, Geor, James, Anthony, Preda, Adrian, Weideman, Andrea, Stein, Dan J., Howells, Fleur, Uhlmann, Anne, Temmingh, Henk, López Jaramillo, Carlos, Díaz Zuluaga, Ana M., Fortea, Laura, Martínez Heras, Eloy, Solana Díaz, Elisabeth, Llufriu Duran, Sara, ENIGMA Consortium, ENIGMA Consortium collaborators
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/219930
Acesso em linha:https://hdl.handle.net/2445/219930
Access Level:acceso abierto
Palavra-chave:Diagnòstic per la imatge
Cervell
Esquizofrènia
Diagnostic imaging
Brain
Schizophrenia
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spelling Increased power by harmonizing structural MRI site differences with the comBat batch adjustment method in ENIGMARadua, JoaquimVieta i Pascual, Eduard, 1963-Shinohara, RussellKochunov, PeterQuidé, YannGreen, Melissa J.Weickert, Cynthia S.Weickert, ThomasBruggemann, JasonKircher, TiloNenadic, IgorCairns, Murray J.Seal, MarcSchall, UlrichHenskens, FransFullerton, Janice M.Mowry, BryanPantelis, ChristosLenroot, RhoshelCropley, VanessaLoughland, CarmelScott, Rodney J.Wolf, DanielSatterthwaite, Theodore D.Tan, YunlongSim, KangPiras, FabrizioSpalletta, GianfrancoBanaj, NerisaPomarol-Clotet, EdithSolanes, AleixAlbajes Eizagirre, AntonCanales Rodríguez, Erick JorgeSarró, SalvadorDi Giorgio, AnnabellaBertolino, AlessandroStäblein, MichaelOertel, ViolaKnöchel, ChristianBorgwardt, Stefandu Plessis, StefanYun, Je-YeonKwon, Jun SooDannlowski, UdoHahn, TimGrotegerd, DominikAlloza, ClaraArango López, CelsoJanssen, JoostDíaz Caneja, Covadonga M.Jiang, WenhaoCalhoun, VinceEhrlich, StefanYang, KunCascella, Nicola G.Takayanagi, YoichiroSawa, AkiraTomyshev, AlexanderLebedeva, IrinaKaleda, VasilyKirschner, MatthiasHoschl, CyrilTomecek, DavidSkoch, Antoninvan Amelsvoort, ThereseBakker, GeorJames, AnthonyPreda, AdrianWeideman, AndreaStein, Dan J.Howells, FleurUhlmann, AnneTemmingh, HenkLópez Jaramillo, CarlosDíaz Zuluaga, Ana M.Fortea, LauraMartínez Heras, EloySolana Díaz, ElisabethLlufriu Duran, SaraENIGMA ConsortiumENIGMA Consortium collaboratorsDiagnòstic per la imatgeCervellEsquizofrèniaDiagnostic imagingBrainSchizophreniaA common limitation of neuroimaging studies is their small sample sizes. To overcome this hurdle, the Enhancing Neuro Imaging Genetics through Meta-Analysis (ENIGMA) Consortium combines neuroimaging data from many institutions worldwide. However, this introduces heterogeneity due to different scanning devices and sequences. ENIGMA projects commonly address this heterogeneity with random-effects meta-analysis or mixed-effects mega-analysis. Here we tested whether the batch adjustment method, ComBat, can further reduce site-related heterogeneity and thus increase statistical power. We conducted random-effects meta-analyses, mixed-effects mega-analyses and ComBat mega-analyses to compare cortical thickness, surface area and subcortical volumes between 2897 individuals with a diagnosis of schizophrenia and 3141 healthy controls from 33 sites. Specifically, we compared the imaging data between individuals with schizophrenia and healthy controls, covarying for age and sex. The use of ComBat substantially increased the statistical significance of the findings as compared to random-effects meta-analyses. The findings were more similar when comparing ComBat with mixed-effects mega-analysis, although ComBat still slightly increased the statistical significance. ComBat also showed increased statistical power when we repeated the analyses with fewer sites. Results were nearly identical when we applied the ComBat harmonization separately for cortical thickness, cortical surface area and subcortical volumes. Therefore, we recommend applying the ComBat function to attenuate potential effects of site in ENIGMA projects and other multi-site structural imaging work. We provide easy-to-use functions in R that work even if imaging data are partially missing in some brain regions, and they can be trained with one data set and then applied to another (a requirement for some analyses such as machine learning).Elsevier B.V.2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/219930Articles publicats en revistes (Medicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.neuroimage.2020.116956Neuroimage, 2020, vol. 218https://doi.org/10.1016/j.neuroimage.2020.116956cc-by-nc-nd (c) Radua, J. et al., 2020http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2199302026-05-27T06:46:51Z
dc.title.none.fl_str_mv Increased power by harmonizing structural MRI site differences with the comBat batch adjustment method in ENIGMA
title Increased power by harmonizing structural MRI site differences with the comBat batch adjustment method in ENIGMA
spellingShingle Increased power by harmonizing structural MRI site differences with the comBat batch adjustment method in ENIGMA
Radua, Joaquim
Diagnòstic per la imatge
Cervell
Esquizofrènia
Diagnostic imaging
Brain
Schizophrenia
title_short Increased power by harmonizing structural MRI site differences with the comBat batch adjustment method in ENIGMA
title_full Increased power by harmonizing structural MRI site differences with the comBat batch adjustment method in ENIGMA
title_fullStr Increased power by harmonizing structural MRI site differences with the comBat batch adjustment method in ENIGMA
title_full_unstemmed Increased power by harmonizing structural MRI site differences with the comBat batch adjustment method in ENIGMA
title_sort Increased power by harmonizing structural MRI site differences with the comBat batch adjustment method in ENIGMA
dc.creator.none.fl_str_mv Radua, Joaquim
Vieta i Pascual, Eduard, 1963-
Shinohara, Russell
Kochunov, Peter
Quidé, Yann
Green, Melissa J.
Weickert, Cynthia S.
Weickert, Thomas
Bruggemann, Jason
Kircher, Tilo
Nenadic, Igor
Cairns, Murray J.
Seal, Marc
Schall, Ulrich
Henskens, Frans
Fullerton, Janice M.
Mowry, Bryan
Pantelis, Christos
Lenroot, Rhoshel
Cropley, Vanessa
Loughland, Carmel
Scott, Rodney J.
Wolf, Daniel
Satterthwaite, Theodore D.
Tan, Yunlong
Sim, Kang
Piras, Fabrizio
Spalletta, Gianfranco
Banaj, Nerisa
Pomarol-Clotet, Edith
Solanes, Aleix
Albajes Eizagirre, Anton
Canales Rodríguez, Erick Jorge
Sarró, Salvador
Di Giorgio, Annabella
Bertolino, Alessandro
Stäblein, Michael
Oertel, Viola
Knöchel, Christian
Borgwardt, Stefan
du Plessis, Stefan
Yun, Je-Yeon
Kwon, Jun Soo
Dannlowski, Udo
Hahn, Tim
Grotegerd, Dominik
Alloza, Clara
Arango López, Celso
Janssen, Joost
Díaz Caneja, Covadonga M.
Jiang, Wenhao
Calhoun, Vince
Ehrlich, Stefan
Yang, Kun
Cascella, Nicola G.
Takayanagi, Yoichiro
Sawa, Akira
Tomyshev, Alexander
Lebedeva, Irina
Kaleda, Vasily
Kirschner, Matthias
Hoschl, Cyril
Tomecek, David
Skoch, Antonin
van Amelsvoort, Therese
Bakker, Geor
James, Anthony
Preda, Adrian
Weideman, Andrea
Stein, Dan J.
Howells, Fleur
Uhlmann, Anne
Temmingh, Henk
López Jaramillo, Carlos
Díaz Zuluaga, Ana M.
Fortea, Laura
Martínez Heras, Eloy
Solana Díaz, Elisabeth
Llufriu Duran, Sara
ENIGMA Consortium
ENIGMA Consortium collaborators
author Radua, Joaquim
author_facet Radua, Joaquim
Vieta i Pascual, Eduard, 1963-
Shinohara, Russell
Kochunov, Peter
Quidé, Yann
Green, Melissa J.
Weickert, Cynthia S.
Weickert, Thomas
Bruggemann, Jason
Kircher, Tilo
Nenadic, Igor
Cairns, Murray J.
Seal, Marc
Schall, Ulrich
Henskens, Frans
Fullerton, Janice M.
Mowry, Bryan
Pantelis, Christos
Lenroot, Rhoshel
Cropley, Vanessa
Loughland, Carmel
Scott, Rodney J.
Wolf, Daniel
Satterthwaite, Theodore D.
Tan, Yunlong
Sim, Kang
Piras, Fabrizio
Spalletta, Gianfranco
Banaj, Nerisa
Pomarol-Clotet, Edith
Solanes, Aleix
Albajes Eizagirre, Anton
Canales Rodríguez, Erick Jorge
Sarró, Salvador
Di Giorgio, Annabella
Bertolino, Alessandro
Stäblein, Michael
Oertel, Viola
Knöchel, Christian
Borgwardt, Stefan
du Plessis, Stefan
Yun, Je-Yeon
Kwon, Jun Soo
Dannlowski, Udo
Hahn, Tim
Grotegerd, Dominik
Alloza, Clara
Arango López, Celso
Janssen, Joost
Díaz Caneja, Covadonga M.
Jiang, Wenhao
Calhoun, Vince
Ehrlich, Stefan
Yang, Kun
Cascella, Nicola G.
Takayanagi, Yoichiro
Sawa, Akira
Tomyshev, Alexander
Lebedeva, Irina
Kaleda, Vasily
Kirschner, Matthias
Hoschl, Cyril
Tomecek, David
Skoch, Antonin
van Amelsvoort, Therese
Bakker, Geor
James, Anthony
Preda, Adrian
Weideman, Andrea
Stein, Dan J.
Howells, Fleur
Uhlmann, Anne
Temmingh, Henk
López Jaramillo, Carlos
Díaz Zuluaga, Ana M.
Fortea, Laura
Martínez Heras, Eloy
Solana Díaz, Elisabeth
Llufriu Duran, Sara
ENIGMA Consortium
ENIGMA Consortium collaborators
author_role author
author2 Vieta i Pascual, Eduard, 1963-
Shinohara, Russell
Kochunov, Peter
Quidé, Yann
Green, Melissa J.
Weickert, Cynthia S.
Weickert, Thomas
Bruggemann, Jason
Kircher, Tilo
Nenadic, Igor
Cairns, Murray J.
Seal, Marc
Schall, Ulrich
Henskens, Frans
Fullerton, Janice M.
Mowry, Bryan
Pantelis, Christos
Lenroot, Rhoshel
Cropley, Vanessa
Loughland, Carmel
Scott, Rodney J.
Wolf, Daniel
Satterthwaite, Theodore D.
Tan, Yunlong
Sim, Kang
Piras, Fabrizio
Spalletta, Gianfranco
Banaj, Nerisa
Pomarol-Clotet, Edith
Solanes, Aleix
Albajes Eizagirre, Anton
Canales Rodríguez, Erick Jorge
Sarró, Salvador
Di Giorgio, Annabella
Bertolino, Alessandro
Stäblein, Michael
Oertel, Viola
Knöchel, Christian
Borgwardt, Stefan
du Plessis, Stefan
Yun, Je-Yeon
Kwon, Jun Soo
Dannlowski, Udo
Hahn, Tim
Grotegerd, Dominik
Alloza, Clara
Arango López, Celso
Janssen, Joost
Díaz Caneja, Covadonga M.
Jiang, Wenhao
Calhoun, Vince
Ehrlich, Stefan
Yang, Kun
Cascella, Nicola G.
Takayanagi, Yoichiro
Sawa, Akira
Tomyshev, Alexander
Lebedeva, Irina
Kaleda, Vasily
Kirschner, Matthias
Hoschl, Cyril
Tomecek, David
Skoch, Antonin
van Amelsvoort, Therese
Bakker, Geor
James, Anthony
Preda, Adrian
Weideman, Andrea
Stein, Dan J.
Howells, Fleur
Uhlmann, Anne
Temmingh, Henk
López Jaramillo, Carlos
Díaz Zuluaga, Ana M.
Fortea, Laura
Martínez Heras, Eloy
Solana Díaz, Elisabeth
Llufriu Duran, Sara
ENIGMA Consortium
ENIGMA Consortium collaborators
author2_role author
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dc.subject.none.fl_str_mv Diagnòstic per la imatge
Cervell
Esquizofrènia
Diagnostic imaging
Brain
Schizophrenia
topic Diagnòstic per la imatge
Cervell
Esquizofrènia
Diagnostic imaging
Brain
Schizophrenia
description A common limitation of neuroimaging studies is their small sample sizes. To overcome this hurdle, the Enhancing Neuro Imaging Genetics through Meta-Analysis (ENIGMA) Consortium combines neuroimaging data from many institutions worldwide. However, this introduces heterogeneity due to different scanning devices and sequences. ENIGMA projects commonly address this heterogeneity with random-effects meta-analysis or mixed-effects mega-analysis. Here we tested whether the batch adjustment method, ComBat, can further reduce site-related heterogeneity and thus increase statistical power. We conducted random-effects meta-analyses, mixed-effects mega-analyses and ComBat mega-analyses to compare cortical thickness, surface area and subcortical volumes between 2897 individuals with a diagnosis of schizophrenia and 3141 healthy controls from 33 sites. Specifically, we compared the imaging data between individuals with schizophrenia and healthy controls, covarying for age and sex. The use of ComBat substantially increased the statistical significance of the findings as compared to random-effects meta-analyses. The findings were more similar when comparing ComBat with mixed-effects mega-analysis, although ComBat still slightly increased the statistical significance. ComBat also showed increased statistical power when we repeated the analyses with fewer sites. Results were nearly identical when we applied the ComBat harmonization separately for cortical thickness, cortical surface area and subcortical volumes. Therefore, we recommend applying the ComBat function to attenuate potential effects of site in ENIGMA projects and other multi-site structural imaging work. We provide easy-to-use functions in R that work even if imaging data are partially missing in some brain regions, and they can be trained with one data set and then applied to another (a requirement for some analyses such as machine learning).
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/219930
url https://hdl.handle.net/2445/219930
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.neuroimage.2020.116956
Neuroimage, 2020, vol. 218
https://doi.org/10.1016/j.neuroimage.2020.116956
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Radua, J. et al., 2020
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Radua, J. et al., 2020
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Medicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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