Generation of induced pluripotent stem cells from human cord blood cells with only two factors:OCT4 and SOX2
Induced pluripotent stem cells (iPSC ) provide an invaluable resource for regenerative medicine as they allow the generation/nof patient-specific progenitors with potential value for cell therapy. However, in many instances, an off-the-shelf approach is/ndesirable, such as for cell therapy of acute...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10230/12432 |
| Acceso en línea: | http://hdl.handle.net/10230/12432 http://dx.doi.org/10.1038/nprot.2010.16 |
| Access Level: | acceso abierto |
| Palabra clave: | Cél·lules mare embrionàries Medicina regenerativa |
| Sumario: | Induced pluripotent stem cells (iPSC ) provide an invaluable resource for regenerative medicine as they allow the generation/nof patient-specific progenitors with potential value for cell therapy. However, in many instances, an off-the-shelf approach is/ndesirable, such as for cell therapy of acute conditions or when the patient’s somatic cells are altered as a consequence of a chronic/ndisease or aging. Cord blood (CB) stem cells appear ideally suited for this purpose as they are young cells expected to carry/nminimal somatic mutations and possess the immunological immaturity of newborn cells; additionally, several hundred thousand/nimmunotyped CB units are readily available through a worldwide network of CB banks. Here we present a detailed protocol for the/nderivation of CB stem cells and how they can be reprogrammed to pluripotency by retroviral transduction with only two factors/n(OCT 4 and SO X2) in 2 weeks and without the need for additional chemical compounds. |
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