Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in Pseudomonas

Este trabajo fue financiado por la beca BIO2012-31634 del Ministerio de Economía y Competitividad de España, y por el Programa de Investigación MICROAMBIENTE-CM de la Comunidad de Madrid. Francisco Martínez-Granero es el beneficiario de un contrato postdoctoral de la Comunidad de Madrid.

Detalles Bibliográficos
Autores: Martínez-Granero, Francisco, Navazo, Ana, Barahona, Emma, Redondo-Nieto, Miguel, González de Heredia, Elena, Baena, Irene, Martín-Martín, Irene, Rivilla, Rafael, Martín, Marta
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
OAI Identifier:oai:burjcdigital.urjc.es:10115/27915
Acceso en línea:https://hdl.handle.net/10115/27915
Access Level:acceso abierto
Palabra clave:PilZ domain
c-di-GMP levels
regulation of swimming motility and biofilm formation
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spelling Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in PseudomonasMartínez-Granero, FranciscoNavazo, AnaBarahona, EmmaRedondo-Nieto, MiguelGonzález de Heredia, ElenaBaena, IreneMartín-Martín, IreneRivilla, RafaelMartín, MartaPilZ domainc-di-GMP levelsregulation of swimming motility and biofilm formationEste trabajo fue financiado por la beca BIO2012-31634 del Ministerio de Economía y Competitividad de España, y por el Programa de Investigación MICROAMBIENTE-CM de la Comunidad de Madrid. Francisco Martínez-Granero es el beneficiario de un contrato postdoctoral de la Comunidad de Madrid.Diguanylate cyclase and phosphodiesterase enzymatic activities control c-di-GMP levels modulating planktonic versus sessile lifestyle behavior in bacteria. The PilZ domain is described as a sensor of c-di-GMP intracellular levels and the proteins containing a PilZ domain represent the best studied class of c-di-GMP receptors forming part of the c-di-GMP signaling cascade. In P. fluorescens F113 we have found two diguanylate cyclases (WspR, SadC) and one phosphodiesterase (BifA) implicated in regulation of swimming motility and biofilm formation. Here we identify a flgZ gene located in a flagellar operon encoding a protein that contains a PilZ domain. Moreover, we show that FlgZ subcellular localization depends on the c-di-GMP intracellular levels. The overexpression analysis of flgZ in P. fluorescens F113 and P. putida KT2440 backgrounds reveal a participation of FlgZ in Pseudomonas swimming motility regulation. Besides, the epistasis of flgZ over wspR and bifA clearly shows that c-di-GMP intracellular levels produced by the enzymatic activity of the diguanylate cyclase WspR and the phosphodiesterase BifA regulates biofilm formation through FlgZ.PLOS202320232014info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10115/27915reponame:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlosinstname:Universidad Rey Juan CarlosInglésAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:burjcdigital.urjc.es:10115/279152026-06-24T12:48:17Z
dc.title.none.fl_str_mv Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in Pseudomonas
title Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in Pseudomonas
spellingShingle Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in Pseudomonas
Martínez-Granero, Francisco
PilZ domain
c-di-GMP levels
regulation of swimming motility and biofilm formation
title_short Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in Pseudomonas
title_full Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in Pseudomonas
title_fullStr Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in Pseudomonas
title_full_unstemmed Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in Pseudomonas
title_sort Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in Pseudomonas
dc.creator.none.fl_str_mv Martínez-Granero, Francisco
Navazo, Ana
Barahona, Emma
Redondo-Nieto, Miguel
González de Heredia, Elena
Baena, Irene
Martín-Martín, Irene
Rivilla, Rafael
Martín, Marta
author Martínez-Granero, Francisco
author_facet Martínez-Granero, Francisco
Navazo, Ana
Barahona, Emma
Redondo-Nieto, Miguel
González de Heredia, Elena
Baena, Irene
Martín-Martín, Irene
Rivilla, Rafael
Martín, Marta
author_role author
author2 Navazo, Ana
Barahona, Emma
Redondo-Nieto, Miguel
González de Heredia, Elena
Baena, Irene
Martín-Martín, Irene
Rivilla, Rafael
Martín, Marta
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PilZ domain
c-di-GMP levels
regulation of swimming motility and biofilm formation
topic PilZ domain
c-di-GMP levels
regulation of swimming motility and biofilm formation
description Este trabajo fue financiado por la beca BIO2012-31634 del Ministerio de Economía y Competitividad de España, y por el Programa de Investigación MICROAMBIENTE-CM de la Comunidad de Madrid. Francisco Martínez-Granero es el beneficiario de un contrato postdoctoral de la Comunidad de Madrid.
publishDate 2014
dc.date.none.fl_str_mv 2014
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10115/27915
url https://hdl.handle.net/10115/27915
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv reponame:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
collection BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869417565275553792
score 15,811543

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