Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19

The underlying mechanisms explaining the differential course of SARS-CoV-2 infection and the potential clinical consequences after COVID-19 resolution have not been fully elucidated. As a dysregulated mitochondrial activity could impair the immune response, we explored long-lasting changes in mitoch...

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Authors: Gómez Delgado, Irene, López Pastor, Andrea R., González Jiménez, Adela, Ramos Acosta, Carlos, Hernández Garate, Yenitzeh, Martínez Micaelo, Neus, Amigó, Núria, Espino Paisán, Laura, Anguita Mandly, Eduardo Luis, Urcelay, Elena
Format: article
Publication Date:2025
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/115373
Online Access:https://hdl.handle.net/20.500.14352/115373
Access Level:Open access
Keyword:616.98:578.834
578.834:616.98
Mitocondria
COVID-19
Linfocitos
Metabolismo
Secuela
Medicina
32 Ciencias Médicas
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oai_identifier_str oai:docta.ucm.es:20.500.14352/115373
network_acronym_str ES
network_name_str España
repository_id_str
spelling Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19Gómez Delgado, IreneLópez Pastor, Andrea R.González Jiménez, AdelaRamos Acosta, CarlosHernández Garate, YenitzehMartínez Micaelo, NeusAmigó, NúriaEspino Paisán, LauraAnguita Mandly, Eduardo LuisUrcelay, Elena616.98:578.834578.834:616.98MitocondriaCOVID-19LinfocitosMetabolismoSecuelaMedicina32 Ciencias MédicasThe underlying mechanisms explaining the differential course of SARS-CoV-2 infection and the potential clinical consequences after COVID-19 resolution have not been fully elucidated. As a dysregulated mitochondrial activity could impair the immune response, we explored long-lasting changes in mitochondrial functionality, circulating cytokine levels, and metabolomic profiles of infected individuals after symptoms resolution, to evaluate whether a complete recovery could be achieved. Results of this pilot study evidenced that different parameters of aerobic respiration in lymphocytes of individuals recuperated from a severe course lagged behind those shown upon mild COVID-19 recovery, in basal conditions and after simulated reinfection, and they also showed altered glycolytic capacity. The severe groups showed trends to enhanced superoxide production in parallel to lower OPA1-S levels. Unbalance of pivotal mitochondrial fusion (MFN2, OPA1) and fission (DRP1, FIS1) proteins was detected, suggesting a disruption in mitochondrial dynamics, as well as a lack of structural integrity in the electron transport chain. In serum, altered cytokine levels of IL-1β, IFN-α2, and IL-27 persisted long after clinical recovery, and growing amounts of the latter after severe infection correlated with lower basal and maximal respiration, ATP production, and glycolytic capacity. Finally, a trend for higher circulating levels of 3-hydroxybutyrate was found in individuals recovered after severe compared to mild course. In summary, long after acute infection, mitochondrial and metabolic changes seem to differ in a situation of full recovery after mild infection versus the one evolving from severe infection.SpringerUniversidad Complutense de Madrid20252025-01-1020252025-01-10journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/115373reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1153732026-06-02T12:44:21Z
dc.title.none.fl_str_mv Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19
title Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19
spellingShingle Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19
Gómez Delgado, Irene
616.98:578.834
578.834:616.98
Mitocondria
COVID-19
Linfocitos
Metabolismo
Secuela
Medicina
32 Ciencias Médicas
title_short Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19
title_full Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19
title_fullStr Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19
title_full_unstemmed Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19
title_sort Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19
dc.creator.none.fl_str_mv Gómez Delgado, Irene
López Pastor, Andrea R.
González Jiménez, Adela
Ramos Acosta, Carlos
Hernández Garate, Yenitzeh
Martínez Micaelo, Neus
Amigó, Núria
Espino Paisán, Laura
Anguita Mandly, Eduardo Luis
Urcelay, Elena
author Gómez Delgado, Irene
author_facet Gómez Delgado, Irene
López Pastor, Andrea R.
González Jiménez, Adela
Ramos Acosta, Carlos
Hernández Garate, Yenitzeh
Martínez Micaelo, Neus
Amigó, Núria
Espino Paisán, Laura
Anguita Mandly, Eduardo Luis
Urcelay, Elena
author_role author
author2 López Pastor, Andrea R.
González Jiménez, Adela
Ramos Acosta, Carlos
Hernández Garate, Yenitzeh
Martínez Micaelo, Neus
Amigó, Núria
Espino Paisán, Laura
Anguita Mandly, Eduardo Luis
Urcelay, Elena
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 616.98:578.834
578.834:616.98
Mitocondria
COVID-19
Linfocitos
Metabolismo
Secuela
Medicina
32 Ciencias Médicas
topic 616.98:578.834
578.834:616.98
Mitocondria
COVID-19
Linfocitos
Metabolismo
Secuela
Medicina
32 Ciencias Médicas
description The underlying mechanisms explaining the differential course of SARS-CoV-2 infection and the potential clinical consequences after COVID-19 resolution have not been fully elucidated. As a dysregulated mitochondrial activity could impair the immune response, we explored long-lasting changes in mitochondrial functionality, circulating cytokine levels, and metabolomic profiles of infected individuals after symptoms resolution, to evaluate whether a complete recovery could be achieved. Results of this pilot study evidenced that different parameters of aerobic respiration in lymphocytes of individuals recuperated from a severe course lagged behind those shown upon mild COVID-19 recovery, in basal conditions and after simulated reinfection, and they also showed altered glycolytic capacity. The severe groups showed trends to enhanced superoxide production in parallel to lower OPA1-S levels. Unbalance of pivotal mitochondrial fusion (MFN2, OPA1) and fission (DRP1, FIS1) proteins was detected, suggesting a disruption in mitochondrial dynamics, as well as a lack of structural integrity in the electron transport chain. In serum, altered cytokine levels of IL-1β, IFN-α2, and IL-27 persisted long after clinical recovery, and growing amounts of the latter after severe infection correlated with lower basal and maximal respiration, ATP production, and glycolytic capacity. Finally, a trend for higher circulating levels of 3-hydroxybutyrate was found in individuals recovered after severe compared to mild course. In summary, long after acute infection, mitochondrial and metabolic changes seem to differ in a situation of full recovery after mild infection versus the one evolving from severe infection.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-10
2025
2025-01-10
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/115373
url https://hdl.handle.net/20.500.14352/115373
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.198674

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