Biodegradation and heart retention of polymeric microparticles in a rat model of myocardial ischemia
Poly-lactide-co-glycolide (PLGA) microparticles emerged as one of the most promising strategies to achieve site-specific drug delivery. Although these microparticles have been demonstrated to be effective in several wound healing models, their potential in cardiac regeneration has not yet been fully...
| Autores: | , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2013 |
| País: | España |
| Recursos: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/37280 |
| Acesso em linha: | https://hdl.handle.net/10171/37280 |
| Access Level: | acceso abierto |
| Palavra-chave: | Myocardial infarction PLGA microparticles Biocompatibility Phagocytic uptake Growth factors |
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Biodegradation and heart retention of polymeric microparticles in a rat model of myocardial ischemiaFormiga, F.R. (Fabio R.)|||/items/ac4aaf2f-6636-445b-943e-2bb14ad3e326Garbayo, E. (Elisa)|||/items/c0fa35e4-82c5-44f7-b300-740027106dbaDíaz-Herráez, P. (Paula)|||/items/b2b37f65-3fe4-464a-942c-bd20de1eb2ccAbizanda-Sarasa, G. (Gloria)|||/items/1e76a0e3-32d0-424e-b58a-d9ddf7a82ef7Simon-Yarza, T. (Teresa)|||/items/8bf17254-4031-4c72-9a3a-9622a60c311fTamayo, E. (Esther)|||/items/10d4cc56-0128-4842-a5fc-2585a32f1e9fProsper-Cardoso, F. (Felipe)|||/items/3d1b0b82-06c3-4e63-8280-e903dc4dc0c1Blanco-Prieto, M.J. (María José)|||/items/93e177db-635f-456f-b672-b79ef8befc40Myocardial infarctionPLGA microparticlesBiocompatibilityPhagocytic uptakeGrowth factorsPoly-lactide-co-glycolide (PLGA) microparticles emerged as one of the most promising strategies to achieve site-specific drug delivery. Although these microparticles have been demonstrated to be effective in several wound healing models, their potential in cardiac regeneration has not yet been fully assessed. The present work sought to explore PLGA microparticles as cardiac drug delivery systems. PLGA microparticles were prepared by Total Recirculation One-Machine System (TROMS) after the formation of a multiple emulsion. Microparticles of different size were prepared and characterized to select the most suitable size for intramyocardial administration. Next, the potential of PLGA microparticles for administration in the heart was assessed in a MI rat model. Particle biodegradation over time and myocardial tissue reaction were studied by routine staining and confocal microscopy. Results showed that microparticles with a diameter of 5 μm were the most compatible with intramyocardial administration in terms of injectability through a 29-gauge needle and tissue response. Particles were present in the heart tissue for up to three months post-implantation and no particle migration towards other solid organs was observed, demonstrating good myocardial retention. CD68 immunolabeling revealed 31%, 47% and below 4% microparticle uptake by macrophages one week, one month and three months after injection, respectively (P<0.001). Taken together, these findings support the feasibility of the developed PLGA microparticles as vehicles for delivering growth factors in the infarcted myocardium.ElsevierDadun. Depósito Académico Digital Universidad de Navarra20152015-01-1520132013-01-0120132013-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfimage/tiffimage/tiffimage/tiffapplication/pdfapplication/pdfimage/tiffhttps://hdl.handle.net/10171/37280reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/372802026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Biodegradation and heart retention of polymeric microparticles in a rat model of myocardial ischemia |
| title |
Biodegradation and heart retention of polymeric microparticles in a rat model of myocardial ischemia |
| spellingShingle |
Biodegradation and heart retention of polymeric microparticles in a rat model of myocardial ischemia Formiga, F.R. (Fabio R.)|||/items/ac4aaf2f-6636-445b-943e-2bb14ad3e326 Myocardial infarction PLGA microparticles Biocompatibility Phagocytic uptake Growth factors |
| title_short |
Biodegradation and heart retention of polymeric microparticles in a rat model of myocardial ischemia |
| title_full |
Biodegradation and heart retention of polymeric microparticles in a rat model of myocardial ischemia |
| title_fullStr |
Biodegradation and heart retention of polymeric microparticles in a rat model of myocardial ischemia |
| title_full_unstemmed |
Biodegradation and heart retention of polymeric microparticles in a rat model of myocardial ischemia |
| title_sort |
Biodegradation and heart retention of polymeric microparticles in a rat model of myocardial ischemia |
| dc.creator.none.fl_str_mv |
Formiga, F.R. (Fabio R.)|||/items/ac4aaf2f-6636-445b-943e-2bb14ad3e326 Garbayo, E. (Elisa)|||/items/c0fa35e4-82c5-44f7-b300-740027106dba Díaz-Herráez, P. (Paula)|||/items/b2b37f65-3fe4-464a-942c-bd20de1eb2cc Abizanda-Sarasa, G. (Gloria)|||/items/1e76a0e3-32d0-424e-b58a-d9ddf7a82ef7 Simon-Yarza, T. (Teresa)|||/items/8bf17254-4031-4c72-9a3a-9622a60c311f Tamayo, E. (Esther)|||/items/10d4cc56-0128-4842-a5fc-2585a32f1e9f Prosper-Cardoso, F. (Felipe)|||/items/3d1b0b82-06c3-4e63-8280-e903dc4dc0c1 Blanco-Prieto, M.J. (María José)|||/items/93e177db-635f-456f-b672-b79ef8befc40 |
| author |
Formiga, F.R. (Fabio R.)|||/items/ac4aaf2f-6636-445b-943e-2bb14ad3e326 |
| author_facet |
Formiga, F.R. (Fabio R.)|||/items/ac4aaf2f-6636-445b-943e-2bb14ad3e326 Garbayo, E. (Elisa)|||/items/c0fa35e4-82c5-44f7-b300-740027106dba Díaz-Herráez, P. (Paula)|||/items/b2b37f65-3fe4-464a-942c-bd20de1eb2cc Abizanda-Sarasa, G. (Gloria)|||/items/1e76a0e3-32d0-424e-b58a-d9ddf7a82ef7 Simon-Yarza, T. (Teresa)|||/items/8bf17254-4031-4c72-9a3a-9622a60c311f Tamayo, E. (Esther)|||/items/10d4cc56-0128-4842-a5fc-2585a32f1e9f Prosper-Cardoso, F. (Felipe)|||/items/3d1b0b82-06c3-4e63-8280-e903dc4dc0c1 Blanco-Prieto, M.J. (María José)|||/items/93e177db-635f-456f-b672-b79ef8befc40 |
| author_role |
author |
| author2 |
Garbayo, E. (Elisa)|||/items/c0fa35e4-82c5-44f7-b300-740027106dba Díaz-Herráez, P. (Paula)|||/items/b2b37f65-3fe4-464a-942c-bd20de1eb2cc Abizanda-Sarasa, G. (Gloria)|||/items/1e76a0e3-32d0-424e-b58a-d9ddf7a82ef7 Simon-Yarza, T. (Teresa)|||/items/8bf17254-4031-4c72-9a3a-9622a60c311f Tamayo, E. (Esther)|||/items/10d4cc56-0128-4842-a5fc-2585a32f1e9f Prosper-Cardoso, F. (Felipe)|||/items/3d1b0b82-06c3-4e63-8280-e903dc4dc0c1 Blanco-Prieto, M.J. (María José)|||/items/93e177db-635f-456f-b672-b79ef8befc40 |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Myocardial infarction PLGA microparticles Biocompatibility Phagocytic uptake Growth factors |
| topic |
Myocardial infarction PLGA microparticles Biocompatibility Phagocytic uptake Growth factors |
| description |
Poly-lactide-co-glycolide (PLGA) microparticles emerged as one of the most promising strategies to achieve site-specific drug delivery. Although these microparticles have been demonstrated to be effective in several wound healing models, their potential in cardiac regeneration has not yet been fully assessed. The present work sought to explore PLGA microparticles as cardiac drug delivery systems. PLGA microparticles were prepared by Total Recirculation One-Machine System (TROMS) after the formation of a multiple emulsion. Microparticles of different size were prepared and characterized to select the most suitable size for intramyocardial administration. Next, the potential of PLGA microparticles for administration in the heart was assessed in a MI rat model. Particle biodegradation over time and myocardial tissue reaction were studied by routine staining and confocal microscopy. Results showed that microparticles with a diameter of 5 μm were the most compatible with intramyocardial administration in terms of injectability through a 29-gauge needle and tissue response. Particles were present in the heart tissue for up to three months post-implantation and no particle migration towards other solid organs was observed, demonstrating good myocardial retention. CD68 immunolabeling revealed 31%, 47% and below 4% microparticle uptake by macrophages one week, one month and three months after injection, respectively (P<0.001). Taken together, these findings support the feasibility of the developed PLGA microparticles as vehicles for delivering growth factors in the infarcted myocardium. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013 2013-01-01 2013 2013-01-01 2015 2015-01-15 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/37280 |
| url |
https://hdl.handle.net/10171/37280 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf image/tiff image/tiff image/tiff application/pdf application/pdf image/tiff |
| dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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1869417545715417088 |
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15,301603 |