Interactions of JAZ repressors with anthocyanin biosynthesis-related transcription factors of fragaria × ananassa

© 2020 by the authors.

Detalles Bibliográficos
Autores: Garrido-Bigotes, Adrián, Torrejón, Marcela, Solano, Roberto, Figueroa, Carlos R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/229263
Acceso en línea:http://hdl.handle.net/10261/229263
Access Level:acceso abierto
Palabra clave:Jasmonates
JA-signaling pathway
JAZ repressors
MBW-complex
MYC2 transcription factor
Strawberry
YABBY genes
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spelling Interactions of JAZ repressors with anthocyanin biosynthesis-related transcription factors of fragaria × ananassaGarrido-Bigotes, AdriánTorrejón, MarcelaSolano, RobertoFigueroa, Carlos R.JasmonatesJA-signaling pathwayJAZ repressorsMBW-complexMYC2 transcription factorStrawberryYABBY genes© 2020 by the authors.Strawberry fruits are rich in flavonoids like proanthocyanidins and anthocyanins. Their biosynthesis and accumulation are controlled by the MYB-bHLH-WD40 (MBW) transcriptional complex, which is mainly formed by basic helix-loop-helix (bHLH) and MYB transcription factors (TFs). In Arabidopsis thaliana both bHLH and MYB TFs are repressed by JASMONATE ZIM-DOMAIN (JAZ) proteins, the key repressors of the jasmonate-signaling pathway. The aim of this research was the characterization of the FaJAZ1/8.1/9/10 proteins and molecular targets of signaling components and anthocyanin biosynthesis-related TFs of Fragaria × ananassa by protein–protein interactions. For this, domain compositions were studied by multiple alignments and phylogenetic analyses, while interactions were analyzed by yeast two-hybrid (Y2H) assays. We detected high conservation of FaJAZ proteins and jasmonate-signaling components, as well as FabHLHs and FaMYB10 TFs. Moreover, we report the F. × ananassa YABBY1 (FaYAB1) TF, which is related to anthocyanin biosynthesis in Arabidopsis, showed high conservation of functional domains. We demonstrated that FaJAZ repressors interacted with F. × ananassa NOVEL INTERACTOR OF JAZ (FaNINJA), FaMYC2, and JASMONATE ASSOCIATED MYC2-LIKE (FaJAM) proteins. Besides, transcription factors of MBW-complex like FabHLH3, FabHLH33, and FaMYB10, together with FaYAB1, were molecular targets of FaJAZ repressors, exhibiting specificity or redundancy of interaction depending on particular FaJAZ protein. Overall, these results suggest that interactions of jasmonate-signaling components are fully conserved, and anthocyanin biosynthesis might be regulated by JAZ repressors in F. × ananassa.This research was funded by the National Research and Development Agency (ANID, Chile) grant FONDECYT/Regular 1181310 to C.R.F. Work in R.S.’s laboratory was funded by the Spanish Ministry of Science, Innovation and Universities grant BIO2019-107012RB (MICINN/FEDER). A.G.-B. and M.T. acknowledge ANID, grants FONDECYT/Postdoctorado 3190894, and FONDECYT/Regular 1180926, respectively.Multidisciplinary Digital Publishing InstituteAgencia Nacional de Investigación y Desarrollo (Chile)Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/229263reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BIO2019-107012RBBIO2019-107012RB/AEI/10.13039/501100011033http://dx.doi.org/10.3390/agronomy10101586Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2292632026-05-22T06:33:51Z
dc.title.none.fl_str_mv Interactions of JAZ repressors with anthocyanin biosynthesis-related transcription factors of fragaria × ananassa
title Interactions of JAZ repressors with anthocyanin biosynthesis-related transcription factors of fragaria × ananassa
spellingShingle Interactions of JAZ repressors with anthocyanin biosynthesis-related transcription factors of fragaria × ananassa
Garrido-Bigotes, Adrián
Jasmonates
JA-signaling pathway
JAZ repressors
MBW-complex
MYC2 transcription factor
Strawberry
YABBY genes
title_short Interactions of JAZ repressors with anthocyanin biosynthesis-related transcription factors of fragaria × ananassa
title_full Interactions of JAZ repressors with anthocyanin biosynthesis-related transcription factors of fragaria × ananassa
title_fullStr Interactions of JAZ repressors with anthocyanin biosynthesis-related transcription factors of fragaria × ananassa
title_full_unstemmed Interactions of JAZ repressors with anthocyanin biosynthesis-related transcription factors of fragaria × ananassa
title_sort Interactions of JAZ repressors with anthocyanin biosynthesis-related transcription factors of fragaria × ananassa
dc.creator.none.fl_str_mv Garrido-Bigotes, Adrián
Torrejón, Marcela
Solano, Roberto
Figueroa, Carlos R.
author Garrido-Bigotes, Adrián
author_facet Garrido-Bigotes, Adrián
Torrejón, Marcela
Solano, Roberto
Figueroa, Carlos R.
author_role author
author2 Torrejón, Marcela
Solano, Roberto
Figueroa, Carlos R.
author2_role author
author
author
dc.contributor.none.fl_str_mv Agencia Nacional de Investigación y Desarrollo (Chile)
Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Jasmonates
JA-signaling pathway
JAZ repressors
MBW-complex
MYC2 transcription factor
Strawberry
YABBY genes
topic Jasmonates
JA-signaling pathway
JAZ repressors
MBW-complex
MYC2 transcription factor
Strawberry
YABBY genes
description © 2020 by the authors.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/229263
url http://hdl.handle.net/10261/229263
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BIO2019-107012RB
BIO2019-107012RB/AEI/10.13039/501100011033
http://dx.doi.org/10.3390/agronomy10101586
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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