Cell signaling and patterning in limb development : analysis in recombinant limbs and by cell grafting
ABSTRACT: Research in limb development implies the study of how complex structures derive from a group of undifferentiated cells. We have focused on the contribution of signaling pathways involved in patterning the limb along the anterior-posterior (AP) and proximal-distal (PD) axes, taking advantag...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis doctoral |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universidad de Cantabria (UC) |
| Repositorio: | UCrea Repositorio Abierto de la Universidad de Cantabria |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unican.es:10902/9752 |
| Acceso en línea: | http://hdl.handle.net/10902/9752 |
| Access Level: | acceso abierto |
| Palabra clave: | Extremidades recombinantes Desarrollo Sonic Hedgehog (SHH) Autónomo Autopodio Recombinant limbs Development Autonomous Autopod |
| Sumario: | ABSTRACT: Research in limb development implies the study of how complex structures derive from a group of undifferentiated cells. We have focused on the contribution of signaling pathways involved in patterning the limb along the anterior-posterior (AP) and proximal-distal (PD) axes, taking advantages of the chicken embryo model. During wing development, anterior limb bud progenitors show a reduced morphogenetic capacity and require signaling from the posterior progenitors (SHH) to survive: deprival of this signal leads increased GLI3R levels and massive apoptosis (Bastida et al., 2004). However, isolated anterior progenitor cells survive and form digits when dissociated and reaggregated forming Recombinant limbs (RLs). Our first goal has been to clarify the reasons for these two highly divergent behaviors. Our second aim has been to unravel the mechanisms driving PD patterning. Grafting young progenitors to the distal environment of older buds shows that an autonomous timing mechanism specifies the positional values of the zeugopod and autopod, providing a complete model of development (Saiz-Lopez et al., 2015). |
|---|