Genes involved in the adhesion and invasion of Arcobacter butzleri

Arcobacter butzleri is a foodborne pathogen that mainly causes enteritis in humans, but the number of cases of bacteraemia has increased in recent years. However, there is still limited knowledge on the pathogenic mechanisms of this bacterium. To investigate how A. butzleri causes disease, single kn...

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Autores: Baztarrika Uria, Itsaso, Wösten, Marc, Alonso Monsalve, Rodrigo, Martínez Ballesteros, Ilargi, Martínez Malax-Echevarría, Irati
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/68632
Acceso en línea:http://hdl.handle.net/10810/68632
Access Level:acceso abierto
Palabra clave:Arcobacter butzleri
adhesion
invasion
culture cells
gene function
mutagenesis
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spelling Genes involved in the adhesion and invasion of Arcobacter butzleriBaztarrika Uria, ItsasoWösten, MarcAlonso Monsalve, RodrigoMartínez Ballesteros, IlargiMartínez Malax-Echevarría, IratiArcobacter butzleriadhesioninvasionculture cellsgene functionmutagenesisArcobacter butzleri is a foodborne pathogen that mainly causes enteritis in humans, but the number of cases of bacteraemia has increased in recent years. However, there is still limited knowledge on the pathogenic mechanisms of this bacterium. To investigate how A. butzleri causes disease, single knockout mutants were constructed in the cadF, ABU_RS00335, ciaB, and flaAB genes, which might be involved in adhesion and invasion properties. These mutants and the isogenic wild-type (WT) were then tested for their ability to adhere and invade human Caco-2 and HT29-MTX cells. The adhesion and invasion of A. butzleri RM4018 strain was also visualized by a Leica CTR 6500 confocal microscope. The adhesion and invasion abilities of mutants lacking the invasion antigen CiaB or a functional flagellum were lower than those of the WTs. However, the extent of the decrease varied depending on the strain and/or cell line. Mutants lacking the fibronectin (FN)-binding protein CadF consistently exhibited reduced abilities, while the inactivation of the other studied FN-binding protein, ABU_RS00335, led to a reduction in only one of the two strains tested. Therefore, the ciaB and flaAB genes appear to be important for A. butzleri adhesion and invasion properties, while cadF appears to be indispensable.This work supported by the University of the Basque Country UPV/EHU [grant number GIU21/021]. I.B was awarded an Ikertalent Ph.D. fellowship by the Basque Government.Elsevier202420242024info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/68632reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.sciencedirect.com/science/article/pii/S0882401024002195info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/3.0/es/© 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by- nc-nd/4.0/).Atribución-NoComercial-SinDerivadas 3.0 Españaoai:addi.ehu.eus:10810/686322026-06-18T09:23:17Z
dc.title.none.fl_str_mv Genes involved in the adhesion and invasion of Arcobacter butzleri
title Genes involved in the adhesion and invasion of Arcobacter butzleri
spellingShingle Genes involved in the adhesion and invasion of Arcobacter butzleri
Baztarrika Uria, Itsaso
Arcobacter butzleri
adhesion
invasion
culture cells
gene function
mutagenesis
title_short Genes involved in the adhesion and invasion of Arcobacter butzleri
title_full Genes involved in the adhesion and invasion of Arcobacter butzleri
title_fullStr Genes involved in the adhesion and invasion of Arcobacter butzleri
title_full_unstemmed Genes involved in the adhesion and invasion of Arcobacter butzleri
title_sort Genes involved in the adhesion and invasion of Arcobacter butzleri
dc.creator.none.fl_str_mv Baztarrika Uria, Itsaso
Wösten, Marc
Alonso Monsalve, Rodrigo
Martínez Ballesteros, Ilargi
Martínez Malax-Echevarría, Irati
author Baztarrika Uria, Itsaso
author_facet Baztarrika Uria, Itsaso
Wösten, Marc
Alonso Monsalve, Rodrigo
Martínez Ballesteros, Ilargi
Martínez Malax-Echevarría, Irati
author_role author
author2 Wösten, Marc
Alonso Monsalve, Rodrigo
Martínez Ballesteros, Ilargi
Martínez Malax-Echevarría, Irati
author2_role author
author
author
author
dc.subject.none.fl_str_mv Arcobacter butzleri
adhesion
invasion
culture cells
gene function
mutagenesis
topic Arcobacter butzleri
adhesion
invasion
culture cells
gene function
mutagenesis
description Arcobacter butzleri is a foodborne pathogen that mainly causes enteritis in humans, but the number of cases of bacteraemia has increased in recent years. However, there is still limited knowledge on the pathogenic mechanisms of this bacterium. To investigate how A. butzleri causes disease, single knockout mutants were constructed in the cadF, ABU_RS00335, ciaB, and flaAB genes, which might be involved in adhesion and invasion properties. These mutants and the isogenic wild-type (WT) were then tested for their ability to adhere and invade human Caco-2 and HT29-MTX cells. The adhesion and invasion of A. butzleri RM4018 strain was also visualized by a Leica CTR 6500 confocal microscope. The adhesion and invasion abilities of mutants lacking the invasion antigen CiaB or a functional flagellum were lower than those of the WTs. However, the extent of the decrease varied depending on the strain and/or cell line. Mutants lacking the fibronectin (FN)-binding protein CadF consistently exhibited reduced abilities, while the inactivation of the other studied FN-binding protein, ABU_RS00335, led to a reduction in only one of the two strains tested. Therefore, the ciaB and flaAB genes appear to be important for A. butzleri adhesion and invasion properties, while cadF appears to be indispensable.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/68632
url http://hdl.handle.net/10810/68632
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0882401024002195
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Atribución-NoComercial-SinDerivadas 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Atribución-NoComercial-SinDerivadas 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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