Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells

Background Conflicting data exist on the role of pulmonary dendritic cells (DCs) and their maturation in patients with chronic obstructive pulmonary disease (COPD). Herein, we investigated whether disease severity and smoking status could affect the distribution and maturation of DCs in lung tissues...

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Autores: Arellano Orden, Elena, Calero Acuña, Carmen, Moreno Mata, Nicolás, Gómez Izquierdo, Lourdes, Sánchez López, Verónica, López Ramírez, Cecilia, López-Villalobos, José Luis, López-Campos Bodineau, José Luis
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/115256
Acceso en línea:https://hdl.handle.net/11441/115256
https://doi.org/10.1371/journal.pone.0152737
Access Level:acceso abierto
Palabra clave:Cigarette
Smoke
Lung
Myeloid Dendritic Cells
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spelling Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic CellsArellano Orden, ElenaCalero Acuña, CarmenMoreno Mata, NicolásGómez Izquierdo, LourdesSánchez López, VerónicaLópez Ramírez, CeciliaLópez-Villalobos, José LuisLópez-Campos Bodineau, José LuisCigaretteSmokeLungMyeloid Dendritic CellsBackground Conflicting data exist on the role of pulmonary dendritic cells (DCs) and their maturation in patients with chronic obstructive pulmonary disease (COPD). Herein, we investigated whether disease severity and smoking status could affect the distribution and maturation of DCs in lung tissues of patients undergoing elective pneumectomy or lobectomy for suspected primary lung cancer. Materials and Methods A total of 75 consecutive patients were included. Spirometry testing was used to identify COPD. Lung parenchyma sections anatomically distant from the primary lesion were examined. We used flow cytometry to identify different DCs subtypes—including BDCA1-positive myeloid DCs (mDCs), BDCA3-positive mDCs, and plasmacytoid DCs (pDCs)—and determine their maturation markers (CD40, CD80, CD83, and CD86) in all participants. We also identified follicular DCs (fDCs), Langerhans DCs (LDCs), and pDCs in 42 patients by immunohistochemistry. Results COPD was diagnosed in 43 patients (16 current smokers and 27 former smokers), whereas the remaining 32 subjects were classified as non-COPD (11 current smokers, 13 former smokers, and 8 never smokers). The number and maturation of DCs did not differ significantly between COPD and non-COPD patients. However, the results of flow cytometry indicated that maturation markers CD40 and CD83 of BDCA1-positive mDCs were significantly decreased in smokers than in non-smokers (P = 0.023 and 0.013, respectively). Immunohistochemistry also revealed a lower number of LDCs in COPD patients than in non-COPD subjects. Conclusions Cigarette smoke, rather than airflow limitation, is the main determinant of impaired DCs maturation in the lung.Instituto de Salud Carlos IIIMinisterio de Economía y CompetitividadFondo de Investigación Sanitaria (project PS09/00826)Public Library of ScienceCirugíaMedicina2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/115256https://doi.org/10.1371/journal.pone.0152737reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPLoS ONE, 11 (4), art. n.0152737.project PS09/00826https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0152737info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1152562026-06-17T12:51:07Z
dc.title.none.fl_str_mv Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
title Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
spellingShingle Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
Arellano Orden, Elena
Cigarette
Smoke
Lung
Myeloid Dendritic Cells
title_short Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
title_full Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
title_fullStr Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
title_full_unstemmed Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
title_sort Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
dc.creator.none.fl_str_mv Arellano Orden, Elena
Calero Acuña, Carmen
Moreno Mata, Nicolás
Gómez Izquierdo, Lourdes
Sánchez López, Verónica
López Ramírez, Cecilia
López-Villalobos, José Luis
López-Campos Bodineau, José Luis
author Arellano Orden, Elena
author_facet Arellano Orden, Elena
Calero Acuña, Carmen
Moreno Mata, Nicolás
Gómez Izquierdo, Lourdes
Sánchez López, Verónica
López Ramírez, Cecilia
López-Villalobos, José Luis
López-Campos Bodineau, José Luis
author_role author
author2 Calero Acuña, Carmen
Moreno Mata, Nicolás
Gómez Izquierdo, Lourdes
Sánchez López, Verónica
López Ramírez, Cecilia
López-Villalobos, José Luis
López-Campos Bodineau, José Luis
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Cirugía
Medicina
dc.subject.none.fl_str_mv Cigarette
Smoke
Lung
Myeloid Dendritic Cells
topic Cigarette
Smoke
Lung
Myeloid Dendritic Cells
description Background Conflicting data exist on the role of pulmonary dendritic cells (DCs) and their maturation in patients with chronic obstructive pulmonary disease (COPD). Herein, we investigated whether disease severity and smoking status could affect the distribution and maturation of DCs in lung tissues of patients undergoing elective pneumectomy or lobectomy for suspected primary lung cancer. Materials and Methods A total of 75 consecutive patients were included. Spirometry testing was used to identify COPD. Lung parenchyma sections anatomically distant from the primary lesion were examined. We used flow cytometry to identify different DCs subtypes—including BDCA1-positive myeloid DCs (mDCs), BDCA3-positive mDCs, and plasmacytoid DCs (pDCs)—and determine their maturation markers (CD40, CD80, CD83, and CD86) in all participants. We also identified follicular DCs (fDCs), Langerhans DCs (LDCs), and pDCs in 42 patients by immunohistochemistry. Results COPD was diagnosed in 43 patients (16 current smokers and 27 former smokers), whereas the remaining 32 subjects were classified as non-COPD (11 current smokers, 13 former smokers, and 8 never smokers). The number and maturation of DCs did not differ significantly between COPD and non-COPD patients. However, the results of flow cytometry indicated that maturation markers CD40 and CD83 of BDCA1-positive mDCs were significantly decreased in smokers than in non-smokers (P = 0.023 and 0.013, respectively). Immunohistochemistry also revealed a lower number of LDCs in COPD patients than in non-COPD subjects. Conclusions Cigarette smoke, rather than airflow limitation, is the main determinant of impaired DCs maturation in the lung.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/115256
https://doi.org/10.1371/journal.pone.0152737
url https://hdl.handle.net/11441/115256
https://doi.org/10.1371/journal.pone.0152737
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS ONE, 11 (4), art. n.0152737.
project PS09/00826
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0152737
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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