Dataset for the paper "Martín, Alba; Giráldez, F. Javier; Montero, O.; Andrés, S. 2022. Dietary administration of L-carnitine during the fattening period of early feed restricted lambs modifies liver transcriptomic and plasma metabolomic profiles. Animal Feed Science and Technology, 292:115426"
[Description of methods used for collection/generation of data] Liver samples stabilized in RNAlater were homogenized using RLT (Qiagen), Lysing Matrix D and a FastPrep-24 instrument (MP Biochemicals); RNA purification was performed with the RNeasy Mini kit and RNase-Free DNase Set kit (Qiagen). &qu...
| Autores: | , , |
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| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/260397 |
| Acceso en línea: | http://hdl.handle.net/10261/260397 |
| Access Level: | acceso abierto |
| Palabra clave: | Nutrional programming Feed restriction Feed efficiency L-carnitine Lamb Liver Transcriptomes http://aims.fao.org/aos/agrovoc/c_92382 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7030 genómica Ganado Ovinos |
| Sumario: | [Description of methods used for collection/generation of data] Liver samples stabilized in RNAlater were homogenized using RLT (Qiagen), Lysing Matrix D and a FastPrep-24 instrument (MP Biochemicals); RNA purification was performed with the RNeasy Mini kit and RNase-Free DNase Set kit (Qiagen). "Poly(A)+ mRNA fraction was isolated from total RNA and cDNA libraries were obtained following Illumina´s recommendations. Briefly, poly(A)+ RNA was isolated on poly-T oligo-attached magnetic beads and chemically fragmented prior to reverse transcription and cDNA generation. The cDNA fragments then go through an end repair process, the addition of a single ‘A’ base to the 3’ end and then ligation of the adapters. Finally, the products are purified and enriched with PCR to create the indexed final double stranded cDNA library. The quality of the libraries was analyzed in Bioanalyzer 2100, High Sensitivity assay; the quantity of the libraries was determined by real-time PCR in LightCycler 480 (Roche). The pool of the libraries was sequenced by paired-end sequencing (100 × 2) in an Illumina HiSeq 2500 instrument." |
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