Translocation events in the evolution of aminoacyl-tRNA synthetases

We have characterized hisS, the gene encoding the histidyl-tRNA synthetase (HisRS) from the tetraodontoid fish Fugu rubripes. The hisS gene is about 3.5 kbp long and contains 13 exons and 12 introns of 172 bp, on average. The Fugu hisS gene encodes a putative protein of 519 amino acids with the thre...

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Authors: Brenner, Sydney, Corrochano Peláez, Luis María
Format: article
Publication Date:1996
Country:España
Institution:Universidad de Sevilla (US)
Repository:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/29249
Online Access:http://hdl.handle.net/11441/29249
https://doi.org/10.1073/pnas.93.16.8485
Access Level:Open access
Keyword:histidyl-tRNA synthetase
Fugu rubripes
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oai_identifier_str oai:idus.us.es:11441/29249
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repository_id_str
spelling Translocation events in the evolution of aminoacyl-tRNA synthetasesBrenner, SydneyCorrochano Peláez, Luis Maríahistidyl-tRNA synthetaseFugu rubripesWe have characterized hisS, the gene encoding the histidyl-tRNA synthetase (HisRS) from the tetraodontoid fish Fugu rubripes. The hisS gene is about 3.5 kbp long and contains 13 exons and 12 introns of 172 bp, on average. The Fugu hisS gene encodes a putative protein of 519 amino acids with the three motifs identified as signatures of class 2 aminoacyl-tRNA synthetases. A model for the shifting of intron 8 between Fugu and hamster is proposed based on the successive appearance of a cryptic splicing site followed by an insertion mutation that created a new acceptor site. In addition, sequence comparisons suggest that the hisS gene has undergone a translocation through the first intron. As a result, the Fugu HisRS has an N-terminal sequence markedly different from that in the human and hamster enzymes. We propose that similar events have been responsible for variations at the N-terminal end of other aminoacyl-tRNA syn- thetases. Our analysis suggests that this involves exchanges through introns of two exons encoding an ancestral 32-amino acid motif.National Academy of SciencesGenética1996info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/29249https://doi.org/10.1073/pnas.93.16.8485reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésProceedings of the National Academy of Sciences of the United States of America, 93(16), 8485-8489http://www.pnas.org/content/93/16/8485.abstracthttp://dx.doi.org/10.1073/pnas.93.16.8485info:eu-repo/semantics/openAccessoai:idus.us.es:11441/292492026-06-17T12:51:07Z
dc.title.none.fl_str_mv Translocation events in the evolution of aminoacyl-tRNA synthetases
title Translocation events in the evolution of aminoacyl-tRNA synthetases
spellingShingle Translocation events in the evolution of aminoacyl-tRNA synthetases
Brenner, Sydney
histidyl-tRNA synthetase
Fugu rubripes
title_short Translocation events in the evolution of aminoacyl-tRNA synthetases
title_full Translocation events in the evolution of aminoacyl-tRNA synthetases
title_fullStr Translocation events in the evolution of aminoacyl-tRNA synthetases
title_full_unstemmed Translocation events in the evolution of aminoacyl-tRNA synthetases
title_sort Translocation events in the evolution of aminoacyl-tRNA synthetases
dc.creator.none.fl_str_mv Brenner, Sydney
Corrochano Peláez, Luis María
author Brenner, Sydney
author_facet Brenner, Sydney
Corrochano Peláez, Luis María
author_role author
author2 Corrochano Peláez, Luis María
author2_role author
dc.contributor.none.fl_str_mv Genética
dc.subject.none.fl_str_mv histidyl-tRNA synthetase
Fugu rubripes
topic histidyl-tRNA synthetase
Fugu rubripes
description We have characterized hisS, the gene encoding the histidyl-tRNA synthetase (HisRS) from the tetraodontoid fish Fugu rubripes. The hisS gene is about 3.5 kbp long and contains 13 exons and 12 introns of 172 bp, on average. The Fugu hisS gene encodes a putative protein of 519 amino acids with the three motifs identified as signatures of class 2 aminoacyl-tRNA synthetases. A model for the shifting of intron 8 between Fugu and hamster is proposed based on the successive appearance of a cryptic splicing site followed by an insertion mutation that created a new acceptor site. In addition, sequence comparisons suggest that the hisS gene has undergone a translocation through the first intron. As a result, the Fugu HisRS has an N-terminal sequence markedly different from that in the human and hamster enzymes. We propose that similar events have been responsible for variations at the N-terminal end of other aminoacyl-tRNA syn- thetases. Our analysis suggests that this involves exchanges through introns of two exons encoding an ancestral 32-amino acid motif.
publishDate 1996
dc.date.none.fl_str_mv 1996
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/29249
https://doi.org/10.1073/pnas.93.16.8485
url http://hdl.handle.net/11441/29249
https://doi.org/10.1073/pnas.93.16.8485
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Proceedings of the National Academy of Sciences of the United States of America, 93(16), 8485-8489
http://www.pnas.org/content/93/16/8485.abstract
http://dx.doi.org/10.1073/pnas.93.16.8485
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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