Impacts of dietary copper on the swine gut microbiome and antibiotic resistome
Restrictions on antibiotic growth promoters have prompted livestock producers to use alternative growth promoters, and dietary copper (Cu) supplementation is currently being widely used in pig production. However, elevated doses of dietary Cu constitute a risk for co-selection of antibiotic resistan...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:269083 |
| Acceso en línea: | https://ddd.uab.cat/record/269083 https://dx.doi.org/urn:doi:10.1016/j.scitotenv.2022.159609 |
| Access Level: | acceso abierto |
| Palabra clave: | Antibiotic resistance genes Antimicrobial resistance Bacterial community composition Co-selection HT-qPCR Pig microbiome |
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Impacts of dietary copper on the swine gut microbiome and antibiotic resistomeBrinck, Julius EmilLassen, Simon BoForouzandeh, Asal|||0000-0002-2436-4007Pan, TingWang, Yan ZiMonteiro, AlessandraBlavi Josa, Laia|||0000-0003-4292-7245Solà Oriol, David|||0000-0001-8365-340XStein, Hans H.|||0000-0002-4855-661XSu, Jian QiangBrandt, Kristian K.Antibiotic resistance genesAntimicrobial resistanceBacterial community compositionCo-selectionHT-qPCRPig microbiomeRestrictions on antibiotic growth promoters have prompted livestock producers to use alternative growth promoters, and dietary copper (Cu) supplementation is currently being widely used in pig production. However, elevated doses of dietary Cu constitute a risk for co-selection of antibiotic resistance and the risk may depend on the type of Cu-based feed additives being used. We here report the first controlled experiment investigating the impact of two contrasting Cu-based feed additives on the overall swine gut microbiome and antibiotic resistome. DNA was extracted from fecal samples (n = 96) collected at four time points during 116 days from 120 pigs allotted to three dietary treatments: control, divalent copper sulfate (CuSO 4; 250 μg Cu g -1 feed), and monovalent copper oxide (Cu 2O; 250 μg Cu g -1 feed). Bacterial community composition, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs) were assessed, and bioavailable Cu ([Cu] bio) was determined using whole-cell bacterial bioreporters. Cu supplementation to feed increased total Cu concentrations ([Cu] total) and [Cu] bio in feces 8-10 fold and at least 670-1000 fold, respectively, but with no significant differences between the two Cu sources. The swine gut microbiome harbored highly abundant and diverse ARGs and MGEs irrespective of the treatments throughout the experiment. Microbiomes differed significantly between pig growth stages and tended to converge over time, but only minor changes in the bacterial community composition and resistome could be linked to Cu supplementation. A significant correlation between bacterial community composition (i.e., bacterial taxa present) and ARG prevalence patterns were observed by Procrustes analysis. Overall, results of the experiment did not provide evidence for Cu-induced co-selection of ARGs or MGEs even at a Cu concentration level exceeding the maximal permitted level for pig diets in the EU (25 to 150 μg Cu g -1 feed depending on age). 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/269083https://dx.doi.org/urn:doi:10.1016/j.scitotenv.2022.159609reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2690832026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Impacts of dietary copper on the swine gut microbiome and antibiotic resistome |
| title |
Impacts of dietary copper on the swine gut microbiome and antibiotic resistome |
| spellingShingle |
Impacts of dietary copper on the swine gut microbiome and antibiotic resistome Brinck, Julius Emil Antibiotic resistance genes Antimicrobial resistance Bacterial community composition Co-selection HT-qPCR Pig microbiome |
| title_short |
Impacts of dietary copper on the swine gut microbiome and antibiotic resistome |
| title_full |
Impacts of dietary copper on the swine gut microbiome and antibiotic resistome |
| title_fullStr |
Impacts of dietary copper on the swine gut microbiome and antibiotic resistome |
| title_full_unstemmed |
Impacts of dietary copper on the swine gut microbiome and antibiotic resistome |
| title_sort |
Impacts of dietary copper on the swine gut microbiome and antibiotic resistome |
| dc.creator.none.fl_str_mv |
Brinck, Julius Emil Lassen, Simon Bo Forouzandeh, Asal|||0000-0002-2436-4007 Pan, Ting Wang, Yan Zi Monteiro, Alessandra Blavi Josa, Laia|||0000-0003-4292-7245 Solà Oriol, David|||0000-0001-8365-340X Stein, Hans H.|||0000-0002-4855-661X Su, Jian Qiang Brandt, Kristian K. |
| author |
Brinck, Julius Emil |
| author_facet |
Brinck, Julius Emil Lassen, Simon Bo Forouzandeh, Asal|||0000-0002-2436-4007 Pan, Ting Wang, Yan Zi Monteiro, Alessandra Blavi Josa, Laia|||0000-0003-4292-7245 Solà Oriol, David|||0000-0001-8365-340X Stein, Hans H.|||0000-0002-4855-661X Su, Jian Qiang Brandt, Kristian K. |
| author_role |
author |
| author2 |
Lassen, Simon Bo Forouzandeh, Asal|||0000-0002-2436-4007 Pan, Ting Wang, Yan Zi Monteiro, Alessandra Blavi Josa, Laia|||0000-0003-4292-7245 Solà Oriol, David|||0000-0001-8365-340X Stein, Hans H.|||0000-0002-4855-661X Su, Jian Qiang Brandt, Kristian K. |
| author2_role |
author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Antibiotic resistance genes Antimicrobial resistance Bacterial community composition Co-selection HT-qPCR Pig microbiome |
| topic |
Antibiotic resistance genes Antimicrobial resistance Bacterial community composition Co-selection HT-qPCR Pig microbiome |
| description |
Restrictions on antibiotic growth promoters have prompted livestock producers to use alternative growth promoters, and dietary copper (Cu) supplementation is currently being widely used in pig production. However, elevated doses of dietary Cu constitute a risk for co-selection of antibiotic resistance and the risk may depend on the type of Cu-based feed additives being used. We here report the first controlled experiment investigating the impact of two contrasting Cu-based feed additives on the overall swine gut microbiome and antibiotic resistome. DNA was extracted from fecal samples (n = 96) collected at four time points during 116 days from 120 pigs allotted to three dietary treatments: control, divalent copper sulfate (CuSO 4; 250 μg Cu g -1 feed), and monovalent copper oxide (Cu 2O; 250 μg Cu g -1 feed). Bacterial community composition, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs) were assessed, and bioavailable Cu ([Cu] bio) was determined using whole-cell bacterial bioreporters. Cu supplementation to feed increased total Cu concentrations ([Cu] total) and [Cu] bio in feces 8-10 fold and at least 670-1000 fold, respectively, but with no significant differences between the two Cu sources. The swine gut microbiome harbored highly abundant and diverse ARGs and MGEs irrespective of the treatments throughout the experiment. Microbiomes differed significantly between pig growth stages and tended to converge over time, but only minor changes in the bacterial community composition and resistome could be linked to Cu supplementation. A significant correlation between bacterial community composition (i.e., bacterial taxa present) and ARG prevalence patterns were observed by Procrustes analysis. Overall, results of the experiment did not provide evidence for Cu-induced co-selection of ARGs or MGEs even at a Cu concentration level exceeding the maximal permitted level for pig diets in the EU (25 to 150 μg Cu g -1 feed depending on age). |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2 2023-01-01 2023 2023-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
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article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/269083 https://dx.doi.org/urn:doi:10.1016/j.scitotenv.2022.159609 |
| url |
https://ddd.uab.cat/record/269083 https://dx.doi.org/urn:doi:10.1016/j.scitotenv.2022.159609 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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