Thermoluminescence can be used to study lipid peroxidation in photosynthetic organisms

Oxidants attack lipids with carbon-carbon double bonds, causing the formation of lipid peroxyl radicals and hydroperoxides through a process called lipid peroxidation. Different aldehydes, including malondialdehyde, can also be formed as secondary products. The thiobarbituric acid reactive substance...

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Detalhes bibliográficos
Autor: Ortega Rodríguez, José María
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2025
País:España
Recursos:Universidad de Sevilla (US)
Repositório:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/179221
Acesso em linha:https://hdl.handle.net/11441/179221
https://doi.org/10.1007/s11120-025-01171-4
Access Level:Acceso aberto
Palavra-chave:Lipid peroxidation
Oxidative stress. Photosynthesis
Thermoluminescence
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spelling Thermoluminescence can be used to study lipid peroxidation in photosynthetic organismsOrtega Rodríguez, José MaríaLipid peroxidationOxidative stress. PhotosynthesisThermoluminescenceOxidants attack lipids with carbon-carbon double bonds, causing the formation of lipid peroxyl radicals and hydroperoxides through a process called lipid peroxidation. Different aldehydes, including malondialdehyde, can also be formed as secondary products. The thiobarbituric acid reactive substances (TBARS) test is commonly used as an assay to measure lipid peroxidation, and its determination is based on spectrophotometric quantification of malondialdehyde. However, the TBARS test is not entirely specific for lipid peroxidation analysis because of the presence of other malondialdehyde sources and the possibility of reaction with other oxidation products. High temperature thermoluminescence technique is a useful method for studying lipid peroxidation in photosynthetic organisms. This technique measures the luminescence emission generated at high temperatures by some of the final products of lipid peroxidation. The breakdown of lipid peroxides is caused by high temperatures, which leads to the formation of carbonyl species in an excited triplet state. When chlorophyll molecules receive energy from excited carbonyls, they release this energy as luminescence once they settle into their ground state. Multiple studies have observed significant thermoluminescence emission bands at high temperatures caused by the energy transfer of lipid peroxidation by-products to chlorophyll. The band peaking at 115–130 °C correlates well with the concentration of different lipid peroxidation products. This band is an extremely sensitive in vivo indicator of the effects of stress conditions in photosynthetic materials. This technique has several benefits when used for lipid peroxidation assays. It is non-invasive, does not require the addition of external probes, and offers sensitive and continuous monitoring of peroxide levels.Springer NatureBioquímica Vegetal y Biología MolecularJunta de AndalucíaJunta de Andalucía2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/179221https://doi.org/10.1007/s11120-025-01171-4reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPhotosynthesis Research, 163 (5), 49.PID2020- 112645GB-100PID2023-146157NB-100PCM 00004https://doi.org/10.1007/s11120-025-01171-4info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1792212026-06-17T12:51:07Z
dc.title.none.fl_str_mv Thermoluminescence can be used to study lipid peroxidation in photosynthetic organisms
title Thermoluminescence can be used to study lipid peroxidation in photosynthetic organisms
spellingShingle Thermoluminescence can be used to study lipid peroxidation in photosynthetic organisms
Ortega Rodríguez, José María
Lipid peroxidation
Oxidative stress. Photosynthesis
Thermoluminescence
title_short Thermoluminescence can be used to study lipid peroxidation in photosynthetic organisms
title_full Thermoluminescence can be used to study lipid peroxidation in photosynthetic organisms
title_fullStr Thermoluminescence can be used to study lipid peroxidation in photosynthetic organisms
title_full_unstemmed Thermoluminescence can be used to study lipid peroxidation in photosynthetic organisms
title_sort Thermoluminescence can be used to study lipid peroxidation in photosynthetic organisms
dc.creator.none.fl_str_mv Ortega Rodríguez, José María
author Ortega Rodríguez, José María
author_facet Ortega Rodríguez, José María
author_role author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
Junta de Andalucía
Junta de Andalucía
dc.subject.none.fl_str_mv Lipid peroxidation
Oxidative stress. Photosynthesis
Thermoluminescence
topic Lipid peroxidation
Oxidative stress. Photosynthesis
Thermoluminescence
description Oxidants attack lipids with carbon-carbon double bonds, causing the formation of lipid peroxyl radicals and hydroperoxides through a process called lipid peroxidation. Different aldehydes, including malondialdehyde, can also be formed as secondary products. The thiobarbituric acid reactive substances (TBARS) test is commonly used as an assay to measure lipid peroxidation, and its determination is based on spectrophotometric quantification of malondialdehyde. However, the TBARS test is not entirely specific for lipid peroxidation analysis because of the presence of other malondialdehyde sources and the possibility of reaction with other oxidation products. High temperature thermoluminescence technique is a useful method for studying lipid peroxidation in photosynthetic organisms. This technique measures the luminescence emission generated at high temperatures by some of the final products of lipid peroxidation. The breakdown of lipid peroxides is caused by high temperatures, which leads to the formation of carbonyl species in an excited triplet state. When chlorophyll molecules receive energy from excited carbonyls, they release this energy as luminescence once they settle into their ground state. Multiple studies have observed significant thermoluminescence emission bands at high temperatures caused by the energy transfer of lipid peroxidation by-products to chlorophyll. The band peaking at 115–130 °C correlates well with the concentration of different lipid peroxidation products. This band is an extremely sensitive in vivo indicator of the effects of stress conditions in photosynthetic materials. This technique has several benefits when used for lipid peroxidation assays. It is non-invasive, does not require the addition of external probes, and offers sensitive and continuous monitoring of peroxide levels.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/179221
https://doi.org/10.1007/s11120-025-01171-4
url https://hdl.handle.net/11441/179221
https://doi.org/10.1007/s11120-025-01171-4
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Photosynthesis Research, 163 (5), 49.
PID2020- 112645GB-100
PID2023-146157NB-100
PCM 00004
https://doi.org/10.1007/s11120-025-01171-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
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