Altered nitrogen metabolism in biocontrol strains of Penicillium rubens

54 p.-5 fig.-5 tab.-4 fig.supl.

Detalles Bibliográficos
Autores: Espeso, Eduardo A., Villarino, María, Carreras, María, Alonso, Lola, Alonso, J. M., Melgarejo, P., Larena, Inmaculada
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/188448
Acceso en línea:http://hdl.handle.net/10261/188448
Access Level:acceso abierto
Palabra clave:Biocontrol agents
CrnA
NirA
Penicillium rubens
Nitrate metabolism
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spelling Altered nitrogen metabolism in biocontrol strains of Penicillium rubensEspeso, Eduardo A.Villarino, MaríaCarreras, MaríaAlonso, LolaAlonso, J. M.Melgarejo, P.Larena, InmaculadaBiocontrol agentsCrnANirAPenicillium rubensNitrate metabolism54 p.-5 fig.-5 tab.-4 fig.supl.The importance of the metabolic route of nitrogen in the fungus Penicillium rubens (strain PO212) is studied in relation to its biocontrol activity (BA). PO212 can resist a high concentration of chlorate anion and displays a classical nitrate-deficiency (nit-) phenotype resulting in poor colonial growth when nitrate is used as the main source of nitrogen. Analyses of genes implicated in nitrate assimilation evidenced the strong sequence conservation of PO212 and CH8 genome with penicillin producers such as reference strain P. rubens Wisconsin 54-1255, P2niaD18 and Pc3, however also revealed the presence of mutations. PO212 carries a mutation in the gene coding for zinc-binuclear cluster transcription factor NirA that specifically mediates the regulation of genes involved in nitrate assimilation. The nirA1 mutation causes an early stop of NirA factor, losing 66% of its sequence. The NirA1 mutant form is unable to mediate a nitrate-dependent regulation of nitrate and nitrite reductase coding genes. In this study, we study another isolate, CH8, with potential BA and nit- phenotype. A mutation in the nitrate permease coding gene crnA was found in CH8. An insertion of a guanine in the coding sequence cause a frameshift in CrnA with the loss of the last two transmembrane domains. Analysis of PO212 and CH8 isolates and complementation strains show the importance of NirA regulator in maintaining correct transcriptional levels of nitrate and nitrite reductases and suggest CrnA as the main nitrate transporter. the presence of alternative transporter for chlorate and the existence of a mechanism for preventing nitrite derived toxicity in Penicillum. BA of PO212 is partially altered when nirA1 mutation was complemented. This result and the finding of CH8, a novel biocontrol P. rubens strain with a nit- phenotype, suggest that nitrogen metabolism is a component of biocontrol capacity.The work at the CIB/CSIC laboratory was supported by the Spanish Ministerio de Economia y Competitividad (MINECO) throught Grants BFU212-33142 and BFU2015- 66806-R, partially supported by the Fondo Europeo de Desarrollo Regional (FEDER, EU), to E.A.E. and at the INIA was funded from RTA2010-00093-00-00, RTA2013- 00060-C05-01, RTA2017-00060-C03-01 (Plan Nacional de I+D, MCINNU, Spain). M. Villarino held postdoctoral contracts that were associated to grants RTA2010-00093-00-00 and BFU2012-33142 from the MINECO, Spain.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Espeso, Eduardo A. [0000-0002-5873-6059]Alonso, Lola [0000-0002-3493-718X]Larena, Inmaculada [0000-0001-8424-8916]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/188448reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015- 66806-Rhttps://doi.org/10.1016/j.fgb.2019.103263Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1884482026-05-22T06:33:51Z
dc.title.none.fl_str_mv Altered nitrogen metabolism in biocontrol strains of Penicillium rubens
title Altered nitrogen metabolism in biocontrol strains of Penicillium rubens
spellingShingle Altered nitrogen metabolism in biocontrol strains of Penicillium rubens
Espeso, Eduardo A.
Biocontrol agents
CrnA
NirA
Penicillium rubens
Nitrate metabolism
title_short Altered nitrogen metabolism in biocontrol strains of Penicillium rubens
title_full Altered nitrogen metabolism in biocontrol strains of Penicillium rubens
title_fullStr Altered nitrogen metabolism in biocontrol strains of Penicillium rubens
title_full_unstemmed Altered nitrogen metabolism in biocontrol strains of Penicillium rubens
title_sort Altered nitrogen metabolism in biocontrol strains of Penicillium rubens
dc.creator.none.fl_str_mv Espeso, Eduardo A.
Villarino, María
Carreras, María
Alonso, Lola
Alonso, J. M.
Melgarejo, P.
Larena, Inmaculada
author Espeso, Eduardo A.
author_facet Espeso, Eduardo A.
Villarino, María
Carreras, María
Alonso, Lola
Alonso, J. M.
Melgarejo, P.
Larena, Inmaculada
author_role author
author2 Villarino, María
Carreras, María
Alonso, Lola
Alonso, J. M.
Melgarejo, P.
Larena, Inmaculada
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Espeso, Eduardo A. [0000-0002-5873-6059]
Alonso, Lola [0000-0002-3493-718X]
Larena, Inmaculada [0000-0001-8424-8916]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biocontrol agents
CrnA
NirA
Penicillium rubens
Nitrate metabolism
topic Biocontrol agents
CrnA
NirA
Penicillium rubens
Nitrate metabolism
description 54 p.-5 fig.-5 tab.-4 fig.supl.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/188448
url http://hdl.handle.net/10261/188448
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015- 66806-R
https://doi.org/10.1016/j.fgb.2019.103263
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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