Temporal profile of the singlet oxygen emission endogenously produced by photosystem II reaction centre in an aqueous buffer.

The temporal profile of the phosphorescence of singlet oxygen endogenously photosensitized by photosystem II (PSII) reaction centre (RC) in an aqueous buffer has been recorded using laser excitation and a near infrared photomultiplier tube. A weak emission signal was discernible, and could be fitted...

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Detalles Bibliográficos
Autores: Li, Heng, Melo, Thor Bernt, Arellano, Juan B., Naqvi, K. Razi
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/48014
Acceso en línea:http://hdl.handle.net/10261/48014
Access Level:acceso abierto
Palabra clave:Singlet oxygen
Photosystem II
Reaction centre
Phosphorescence emission
temporal profile
Aqueous medium
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spelling Temporal profile of the singlet oxygen emission endogenously produced by photosystem II reaction centre in an aqueous buffer.Li, HengMelo, Thor BerntArellano, Juan B.Naqvi, K. RaziSinglet oxygenPhotosystem IIReaction centrePhosphorescence emissiontemporal profileAqueous mediumThe temporal profile of the phosphorescence of singlet oxygen endogenously photosensitized by photosystem II (PSII) reaction centre (RC) in an aqueous buffer has been recorded using laser excitation and a near infrared photomultiplier tube. A weak emission signal was discernible, and could be fitted to the functional form a[exp(-t/tau2)-exp(-t/tau1), with a > 0 and tau2 > tau1. The value of tau2 decreased from 11.6+/-0.5μs under aerobic conditions to 4.1+/-0.2 μs in oxygen saturated samples, due to enhanced bimolecular quenching of the donor triplet by oxygen, whereas that of tau1, identifiable with the lifetime of singlet oxygen, was close to 3 μs in both cases. Extrapolations based on the low amplitude of the emission signal of singlet oxygen formed by PSII RC in the aqueous buffer and the expected values of tau1 and tau2 in chloroplasts indicate that attempts to analyse the temporal profile of singlet oxygen in chloroplasts are unlikely to be rewarded with success without a significant advance in the sensitivity of the detection equipment.This work was funded by the Research Council of Norway (Project No. 191102) and the Spanish Ministry of Science and Innovation (Grant number BFU2007-68107-C02-02/BMC). JBA is very grateful to the CSIC mobility programme for funding his short term visit to NTNU (Ref. number PA1002668).Peer reviewedSpringer Nature201220122012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/48014reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www.springerlink.com/content/h12281214238n617/info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/480142026-05-22T06:33:51Z
dc.title.none.fl_str_mv Temporal profile of the singlet oxygen emission endogenously produced by photosystem II reaction centre in an aqueous buffer.
title Temporal profile of the singlet oxygen emission endogenously produced by photosystem II reaction centre in an aqueous buffer.
spellingShingle Temporal profile of the singlet oxygen emission endogenously produced by photosystem II reaction centre in an aqueous buffer.
Li, Heng
Singlet oxygen
Photosystem II
Reaction centre
Phosphorescence emission
temporal profile
Aqueous medium
title_short Temporal profile of the singlet oxygen emission endogenously produced by photosystem II reaction centre in an aqueous buffer.
title_full Temporal profile of the singlet oxygen emission endogenously produced by photosystem II reaction centre in an aqueous buffer.
title_fullStr Temporal profile of the singlet oxygen emission endogenously produced by photosystem II reaction centre in an aqueous buffer.
title_full_unstemmed Temporal profile of the singlet oxygen emission endogenously produced by photosystem II reaction centre in an aqueous buffer.
title_sort Temporal profile of the singlet oxygen emission endogenously produced by photosystem II reaction centre in an aqueous buffer.
dc.creator.none.fl_str_mv Li, Heng
Melo, Thor Bernt
Arellano, Juan B.
Naqvi, K. Razi
author Li, Heng
author_facet Li, Heng
Melo, Thor Bernt
Arellano, Juan B.
Naqvi, K. Razi
author_role author
author2 Melo, Thor Bernt
Arellano, Juan B.
Naqvi, K. Razi
author2_role author
author
author
dc.subject.none.fl_str_mv Singlet oxygen
Photosystem II
Reaction centre
Phosphorescence emission
temporal profile
Aqueous medium
topic Singlet oxygen
Photosystem II
Reaction centre
Phosphorescence emission
temporal profile
Aqueous medium
description The temporal profile of the phosphorescence of singlet oxygen endogenously photosensitized by photosystem II (PSII) reaction centre (RC) in an aqueous buffer has been recorded using laser excitation and a near infrared photomultiplier tube. A weak emission signal was discernible, and could be fitted to the functional form a[exp(-t/tau2)-exp(-t/tau1), with a > 0 and tau2 > tau1. The value of tau2 decreased from 11.6+/-0.5μs under aerobic conditions to 4.1+/-0.2 μs in oxygen saturated samples, due to enhanced bimolecular quenching of the donor triplet by oxygen, whereas that of tau1, identifiable with the lifetime of singlet oxygen, was close to 3 μs in both cases. Extrapolations based on the low amplitude of the emission signal of singlet oxygen formed by PSII RC in the aqueous buffer and the expected values of tau1 and tau2 in chloroplasts indicate that attempts to analyse the temporal profile of singlet oxygen in chloroplasts are unlikely to be rewarded with success without a significant advance in the sensitivity of the detection equipment.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/48014
url http://hdl.handle.net/10261/48014
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.springerlink.com/content/h12281214238n617/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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