Proteomic Characterization of Urinary Extracellular Vesicles from Kidney-Transplanted Patients Treated with Calcineurin Inhibitors

Use of immunosuppressive drugs is still unavoidable in kidney-transplanted patients. Since their discovery, calcineurin inhibitors (CNI) have been considered the first-line immunosuppressive agents, in spite of their known nephrotoxicity. Chronic CNI toxicity (CNIT) may lead to kidney fibrosis, a th...

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Autores: Carreras-Planella, Laura|||0000-0003-3512-0849, Juega-Mariño, Javier|||0000-0002-9927-9683, Taco, Omar|||0000-0001-9081-5665, Cañas, Laura, Franquesa, Marcella|||0000-0002-1287-8908, Lauzurica, Ricardo|||0000-0002-8051-5199, Borràs i Serres, Francesc Enric|||0000-0003-4038-1912
Tipo de documento: artigo
Data de publicação:2020
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:252995
Acesso em linha:https://ddd.uab.cat/record/252995
https://dx.doi.org/urn:doi:10.3390/ijms21207569
Access Level:Acceso aberto
Palavra-chave:Exosomes
Renal transplantation
Tacrolimus
Cyclosporine A
Proteomics
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spelling Proteomic Characterization of Urinary Extracellular Vesicles from Kidney-Transplanted Patients Treated with Calcineurin InhibitorsCarreras-Planella, Laura|||0000-0003-3512-0849Juega-Mariño, Javier|||0000-0002-9927-9683Taco, Omar|||0000-0001-9081-5665Cañas, LauraFranquesa, Marcella|||0000-0002-1287-8908Lauzurica, Ricardo|||0000-0002-8051-5199Borràs i Serres, Francesc Enric|||0000-0003-4038-1912ExosomesRenal transplantationTacrolimusCyclosporine AProteomicsUse of immunosuppressive drugs is still unavoidable in kidney-transplanted patients. Since their discovery, calcineurin inhibitors (CNI) have been considered the first-line immunosuppressive agents, in spite of their known nephrotoxicity. Chronic CNI toxicity (CNIT) may lead to kidney fibrosis, a threatening scenario for graft survival. However, there is still controversy regarding CNIT diagnosis, monitoring and therapeutic management, and their specific effects at the molecular level are not fully known. Aiming to better characterize CNIT patients, in the present study, we collected urine from kidney-transplanted patients treated with CNI who (i) had a normal kidney function, (ii) suffered CNIT, or (iii) presented interstitial fibrosis and tubular atrophy (IFTA). Urinary extracellular vesicles (uEV) were enriched and the proteome was analyzed to get insight into changes happening during CNI. Members of the uroplakin and plakin families were significantly upregulated in the CNIT group, suggesting an important role in CNIT processes. Although biomarkers cannot be asserted from this single pilot study, our results evidence the potential of uEV as a source of non-invasive protein biomarkers for a better detection and monitoring of this renal alteration in kidney-transplanted patients.Universitat Autònoma de Barcelona. Departament de Medicina 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/252995https://dx.doi.org/urn:doi:10.3390/ijms21207569reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0009"la Caixa" Foundation https://doi.org/10.13039/100010434 LCF/TR/CI19/52460021open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2529952026-06-06T12:50:31Z
dc.title.none.fl_str_mv Proteomic Characterization of Urinary Extracellular Vesicles from Kidney-Transplanted Patients Treated with Calcineurin Inhibitors
title Proteomic Characterization of Urinary Extracellular Vesicles from Kidney-Transplanted Patients Treated with Calcineurin Inhibitors
spellingShingle Proteomic Characterization of Urinary Extracellular Vesicles from Kidney-Transplanted Patients Treated with Calcineurin Inhibitors
Carreras-Planella, Laura|||0000-0003-3512-0849
Exosomes
Renal transplantation
Tacrolimus
Cyclosporine A
Proteomics
title_short Proteomic Characterization of Urinary Extracellular Vesicles from Kidney-Transplanted Patients Treated with Calcineurin Inhibitors
title_full Proteomic Characterization of Urinary Extracellular Vesicles from Kidney-Transplanted Patients Treated with Calcineurin Inhibitors
title_fullStr Proteomic Characterization of Urinary Extracellular Vesicles from Kidney-Transplanted Patients Treated with Calcineurin Inhibitors
title_full_unstemmed Proteomic Characterization of Urinary Extracellular Vesicles from Kidney-Transplanted Patients Treated with Calcineurin Inhibitors
title_sort Proteomic Characterization of Urinary Extracellular Vesicles from Kidney-Transplanted Patients Treated with Calcineurin Inhibitors
dc.creator.none.fl_str_mv Carreras-Planella, Laura|||0000-0003-3512-0849
Juega-Mariño, Javier|||0000-0002-9927-9683
Taco, Omar|||0000-0001-9081-5665
Cañas, Laura
Franquesa, Marcella|||0000-0002-1287-8908
Lauzurica, Ricardo|||0000-0002-8051-5199
Borràs i Serres, Francesc Enric|||0000-0003-4038-1912
author Carreras-Planella, Laura|||0000-0003-3512-0849
author_facet Carreras-Planella, Laura|||0000-0003-3512-0849
Juega-Mariño, Javier|||0000-0002-9927-9683
Taco, Omar|||0000-0001-9081-5665
Cañas, Laura
Franquesa, Marcella|||0000-0002-1287-8908
Lauzurica, Ricardo|||0000-0002-8051-5199
Borràs i Serres, Francesc Enric|||0000-0003-4038-1912
author_role author
author2 Juega-Mariño, Javier|||0000-0002-9927-9683
Taco, Omar|||0000-0001-9081-5665
Cañas, Laura
Franquesa, Marcella|||0000-0002-1287-8908
Lauzurica, Ricardo|||0000-0002-8051-5199
Borràs i Serres, Francesc Enric|||0000-0003-4038-1912
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona. Departament de Medicina
dc.subject.none.fl_str_mv Exosomes
Renal transplantation
Tacrolimus
Cyclosporine A
Proteomics
topic Exosomes
Renal transplantation
Tacrolimus
Cyclosporine A
Proteomics
description Use of immunosuppressive drugs is still unavoidable in kidney-transplanted patients. Since their discovery, calcineurin inhibitors (CNI) have been considered the first-line immunosuppressive agents, in spite of their known nephrotoxicity. Chronic CNI toxicity (CNIT) may lead to kidney fibrosis, a threatening scenario for graft survival. However, there is still controversy regarding CNIT diagnosis, monitoring and therapeutic management, and their specific effects at the molecular level are not fully known. Aiming to better characterize CNIT patients, in the present study, we collected urine from kidney-transplanted patients treated with CNI who (i) had a normal kidney function, (ii) suffered CNIT, or (iii) presented interstitial fibrosis and tubular atrophy (IFTA). Urinary extracellular vesicles (uEV) were enriched and the proteome was analyzed to get insight into changes happening during CNI. Members of the uroplakin and plakin families were significantly upregulated in the CNIT group, suggesting an important role in CNIT processes. Although biomarkers cannot be asserted from this single pilot study, our results evidence the potential of uEV as a source of non-invasive protein biomarkers for a better detection and monitoring of this renal alteration in kidney-transplanted patients.
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/252995
https://dx.doi.org/urn:doi:10.3390/ijms21207569
url https://ddd.uab.cat/record/252995
https://dx.doi.org/urn:doi:10.3390/ijms21207569
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0009
"la Caixa" Foundation https://doi.org/10.13039/100010434 LCF/TR/CI19/52460021
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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