Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses

Objective The aim of this study was to perform a comprehensive assessment of the placental aging process through senescence and apoptotic markers in late-onset small fetuses classified as SGA or FGR. Study Design A prospective nested case-control study in singleton pregnancies delivering at term inc...

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Autores: Paules, Cristina, Dantas, Ana Paula, Miranda, Jezid, Crovetto, Francesca, Eixarch Roca, Elisenda, Rodríguez Sureda, Víctor, Domínguez, Carmen, Casu, Giulia, Rovira, Carlota, Nadal Serra, Alfons, Crispi Brillas, Fàtima, Gratacós Solsona, Eduard
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/125602
Acceso en línea:https://hdl.handle.net/2445/125602
Access Level:acceso abierto
Palabra clave:Creixement fetal
Placenta
Telòmer
Fetal growth
Telomere
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spelling Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetusesPaules, CristinaDantas, Ana PaulaMiranda, JezidCrovetto, FrancescaEixarch Roca, ElisendaRodríguez Sureda, VíctorDomínguez, CarmenCasu, GiuliaRovira, CarlotaNadal Serra, AlfonsCrispi Brillas, FàtimaGratacós Solsona, EduardCreixement fetalPlacentaTelòmerFetal growthPlacentaTelomereObjective The aim of this study was to perform a comprehensive assessment of the placental aging process through senescence and apoptotic markers in late-onset small fetuses classified as SGA or FGR. Study Design A prospective nested case-control study in singleton pregnancies delivering at term including 21 normally grown fetuses and 36 small fetuses classified into SGA (if birthweight was between the 3rd and 9th centile and normal fetoplacental Doppler; n=18) and FGR (if birthweight <3rd centile and/or abnormal cerebroplacental ratio or uterine artery Doppler; n=18). Telomerase activity, telomere length and RNA expression of senescence (Sirtuin 1,3,6) and apoptotic markers (p53, p21, BAX, Caspase 3 and 9) were analyzed in placental samples collected at birth. Results Compared with normally grown fetuses, both SGA and FGR presented signs of accelerated placental aging including lower telomerase activity (controls mean±SD 12.8% ± 6.6 vs SGA 7.98% ± 4.2 vs FGR 7.79% ± 4.6, p=0.008), shorter telomeres (controls 1.20 T/S ± 0.6 vs SGA 1.08 T/S ± 0.9 vs FGR 0.66 T/S ± 0.5, p=0.017), and reduced Sirtuin1 RNA expression (controls 1.55 2-' ' Ct ± 0.8 vs SGA 0.91 2-' ' Ct ± 0.8vs FGR 0.63 2-' ' Ct ± 0.5, p<0.001) together with increased p53 RNA expression (controls median(IQR) 1.072-' ' Ct (3.2) vs SGA 5.39 2-' ' Ct (15) vs FGR 3.75 2-' ' Ct (7.8), p=0.040), with a significant linear tendency across severity stages. In addition, FGR cases presented signs of apoptosis with increased RNA levels of Caspase 3 (controls 0.94 2-' ' Ct (1.1) vs FGR 3.98 2-' ' Ct (30), p=0.031) and Caspase 9 (controls 1.21 2-' ' Ct (4.0) vs FGR 3.87 2-' ' Ct (8.7), p=0.037) as compared to controls.John Wiley & Sons2018201920192018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion28 p.application/pdfhttps://hdl.handle.net/2445/125602Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1002/uog.20103Ultrasound in Obstetrics & Gynecology, 2019, vol. 53, num. 5, p. 615-622https://doi.org/10.1002/uog.20103(c) International Society of Ultrasound in Obstetrics & Gynecology (ISUOG), 2019info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1256022026-05-29T05:05:01Z
dc.title.none.fl_str_mv Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses
title Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses
spellingShingle Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses
Paules, Cristina
Creixement fetal
Placenta
Telòmer
Fetal growth
Placenta
Telomere
title_short Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses
title_full Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses
title_fullStr Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses
title_full_unstemmed Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses
title_sort Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses
dc.creator.none.fl_str_mv Paules, Cristina
Dantas, Ana Paula
Miranda, Jezid
Crovetto, Francesca
Eixarch Roca, Elisenda
Rodríguez Sureda, Víctor
Domínguez, Carmen
Casu, Giulia
Rovira, Carlota
Nadal Serra, Alfons
Crispi Brillas, Fàtima
Gratacós Solsona, Eduard
author Paules, Cristina
author_facet Paules, Cristina
Dantas, Ana Paula
Miranda, Jezid
Crovetto, Francesca
Eixarch Roca, Elisenda
Rodríguez Sureda, Víctor
Domínguez, Carmen
Casu, Giulia
Rovira, Carlota
Nadal Serra, Alfons
Crispi Brillas, Fàtima
Gratacós Solsona, Eduard
author_role author
author2 Dantas, Ana Paula
Miranda, Jezid
Crovetto, Francesca
Eixarch Roca, Elisenda
Rodríguez Sureda, Víctor
Domínguez, Carmen
Casu, Giulia
Rovira, Carlota
Nadal Serra, Alfons
Crispi Brillas, Fàtima
Gratacós Solsona, Eduard
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Creixement fetal
Placenta
Telòmer
Fetal growth
Placenta
Telomere
topic Creixement fetal
Placenta
Telòmer
Fetal growth
Placenta
Telomere
description Objective The aim of this study was to perform a comprehensive assessment of the placental aging process through senescence and apoptotic markers in late-onset small fetuses classified as SGA or FGR. Study Design A prospective nested case-control study in singleton pregnancies delivering at term including 21 normally grown fetuses and 36 small fetuses classified into SGA (if birthweight was between the 3rd and 9th centile and normal fetoplacental Doppler; n=18) and FGR (if birthweight <3rd centile and/or abnormal cerebroplacental ratio or uterine artery Doppler; n=18). Telomerase activity, telomere length and RNA expression of senescence (Sirtuin 1,3,6) and apoptotic markers (p53, p21, BAX, Caspase 3 and 9) were analyzed in placental samples collected at birth. Results Compared with normally grown fetuses, both SGA and FGR presented signs of accelerated placental aging including lower telomerase activity (controls mean±SD 12.8% ± 6.6 vs SGA 7.98% ± 4.2 vs FGR 7.79% ± 4.6, p=0.008), shorter telomeres (controls 1.20 T/S ± 0.6 vs SGA 1.08 T/S ± 0.9 vs FGR 0.66 T/S ± 0.5, p=0.017), and reduced Sirtuin1 RNA expression (controls 1.55 2-' ' Ct ± 0.8 vs SGA 0.91 2-' ' Ct ± 0.8vs FGR 0.63 2-' ' Ct ± 0.5, p<0.001) together with increased p53 RNA expression (controls median(IQR) 1.072-' ' Ct (3.2) vs SGA 5.39 2-' ' Ct (15) vs FGR 3.75 2-' ' Ct (7.8), p=0.040), with a significant linear tendency across severity stages. In addition, FGR cases presented signs of apoptosis with increased RNA levels of Caspase 3 (controls 0.94 2-' ' Ct (1.1) vs FGR 3.98 2-' ' Ct (30), p=0.031) and Caspase 9 (controls 1.21 2-' ' Ct (4.0) vs FGR 3.87 2-' ' Ct (8.7), p=0.037) as compared to controls.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/125602
url https://hdl.handle.net/2445/125602
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1002/uog.20103
Ultrasound in Obstetrics & Gynecology, 2019, vol. 53, num. 5, p. 615-622
https://doi.org/10.1002/uog.20103
dc.rights.none.fl_str_mv (c) International Society of Ultrasound in Obstetrics & Gynecology (ISUOG), 2019
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) International Society of Ultrasound in Obstetrics & Gynecology (ISUOG), 2019
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 28 p.
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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