Exploring Polar Headgroup Interactions Between Sphingomyelin and Ceramide with Infrared Spectroscopy

Ceramide is a major actor in the sphingolipid signaling pathway elicited by various kinds of cell stress. Under those conditions ceramide (Cer) is produced in the plasma membrane as a product of sphingomyelin (SM) hydrolysis, and this may lead to apoptosis. Thus, SM and Cer coexist in the membrane f...

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Autores: De la Arada Echevarría, Igor, González Ramírez, Emilio José, Alonso Izquierdo, Alicia, Goñi Urcelay, Félix María, Rodríguez Arrondo, José Luis
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/50110
Acceso en línea:http://hdl.handle.net/10810/50110
Access Level:acceso abierto
Palabra clave:lipid interactions
phase
sphingolipids
membranes
domains
cholesterol
coexistence
monolayers
proteins
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spelling Exploring Polar Headgroup Interactions Between Sphingomyelin and Ceramide with Infrared SpectroscopyDe la Arada Echevarría, IgorGonzález Ramírez, Emilio JoséAlonso Izquierdo, AliciaGoñi Urcelay, Félix MaríaRodríguez Arrondo, José Luislipid interactionsphasesphingolipidsmembranesdomainscholesterolcoexistencemonolayersproteinsCeramide is a major actor in the sphingolipid signaling pathway elicited by various kinds of cell stress. Under those conditions ceramide (Cer) is produced in the plasma membrane as a product of sphingomyelin (SM) hydrolysis, and this may lead to apoptosis. Thus, SM and Cer coexist in the membrane for some time, and they are known to separate laterally from the (more abundant) glycerolipids, giving rise to highly rigid domains or platforms. The properties of these domains/platforms are rather well understood, but the underlying SM:Cer molecular interactions have not been explored in detail. Infrared (IR) spectroscopy is a powerful analytical technique that provides information on all the chemical groupings in a molecule, and that can be applied to membranes and lipid bilayers in aqueous media. IR spectra can be conveniently retrieved as a function of temperature, thus revealing the thermotropic transitions of SM and its mixtures with Cer. Four regions of the IR spectrum of these sphingolipids have been examined, two of them dominated by the hydrophobic regions in the molecules, namely the C-H stretching vibrations (2800-3000 cm(-1)), and the CH2 scissoring vibrations (1455-1485 cm(-1)), and two others arising from chemical groups at the lipid-water interface, the sphingolipid amide I band (1600-1680 cm(-1)), and the phosphate vibrations in the 1000-1110 cm(-1) region. The latter two regions have been rarely studied in the past. The IR data from the hydrophobic components show a gel (or ripple)-fluid transition of SM at 40 degrees C, that is shifted up to about 70 degrees C when Cer is added to the bilayers, in agreement with previous studies using a variety of techniques. IR information concerning the polar parts is more interesting. The amide I (carbonyl) band of pure SM exhibits a maximum at 1638 cm(-1) at room temperature, and its position is shifted by about 10 cm(-1) in the presence of Cer. Cer causes also a change in the overall band shape, but no signs of band splitting are seen, suggesting that SM and Cer carbonyl groups are interacting tightly, presumably through H-bonds. The 1086 cm(-1) band, corresponding to PO2- vibrations, appears more stable in SM than in DPPC, and it is further stabilized by Cer, again suggesting an important role of H-bonds in the formation of SM:Cer clusters. Thus, SM and Cer can interact through their polar headgroups, in a way that is not accessible to other lipid classes.This work was supported in part by the Spanish Ministerio de Ciencia e Innovacion (MCI), Agencia Estatal de Investigacion (AEI) and Fondo Europeo de Desarrollo Regional (FEDER) (grant No. PGC2018-099857-B-I00), by the Basque Government (Grants No. IT1264-19, IT1196-19 and IT1270-19), by the Fundacion Biofisica Bizkaia and by the Basque Excellence Research Centre (BERC) program of the Basque Government.Nature202120212020info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/50110reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MICINN/PGC2018-099857-B-I00/https://www.nature.com/articles/s41598-020-74781-8info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/Tis article is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0)Atribución 3.0 Españaoai:addi.ehu.eus:10810/501102026-06-18T09:23:17Z
dc.title.none.fl_str_mv Exploring Polar Headgroup Interactions Between Sphingomyelin and Ceramide with Infrared Spectroscopy
title Exploring Polar Headgroup Interactions Between Sphingomyelin and Ceramide with Infrared Spectroscopy
spellingShingle Exploring Polar Headgroup Interactions Between Sphingomyelin and Ceramide with Infrared Spectroscopy
De la Arada Echevarría, Igor
lipid interactions
phase
sphingolipids
membranes
domains
cholesterol
coexistence
monolayers
proteins
title_short Exploring Polar Headgroup Interactions Between Sphingomyelin and Ceramide with Infrared Spectroscopy
title_full Exploring Polar Headgroup Interactions Between Sphingomyelin and Ceramide with Infrared Spectroscopy
title_fullStr Exploring Polar Headgroup Interactions Between Sphingomyelin and Ceramide with Infrared Spectroscopy
title_full_unstemmed Exploring Polar Headgroup Interactions Between Sphingomyelin and Ceramide with Infrared Spectroscopy
title_sort Exploring Polar Headgroup Interactions Between Sphingomyelin and Ceramide with Infrared Spectroscopy
dc.creator.none.fl_str_mv De la Arada Echevarría, Igor
González Ramírez, Emilio José
Alonso Izquierdo, Alicia
Goñi Urcelay, Félix María
Rodríguez Arrondo, José Luis
author De la Arada Echevarría, Igor
author_facet De la Arada Echevarría, Igor
González Ramírez, Emilio José
Alonso Izquierdo, Alicia
Goñi Urcelay, Félix María
Rodríguez Arrondo, José Luis
author_role author
author2 González Ramírez, Emilio José
Alonso Izquierdo, Alicia
Goñi Urcelay, Félix María
Rodríguez Arrondo, José Luis
author2_role author
author
author
author
dc.subject.none.fl_str_mv lipid interactions
phase
sphingolipids
membranes
domains
cholesterol
coexistence
monolayers
proteins
topic lipid interactions
phase
sphingolipids
membranes
domains
cholesterol
coexistence
monolayers
proteins
description Ceramide is a major actor in the sphingolipid signaling pathway elicited by various kinds of cell stress. Under those conditions ceramide (Cer) is produced in the plasma membrane as a product of sphingomyelin (SM) hydrolysis, and this may lead to apoptosis. Thus, SM and Cer coexist in the membrane for some time, and they are known to separate laterally from the (more abundant) glycerolipids, giving rise to highly rigid domains or platforms. The properties of these domains/platforms are rather well understood, but the underlying SM:Cer molecular interactions have not been explored in detail. Infrared (IR) spectroscopy is a powerful analytical technique that provides information on all the chemical groupings in a molecule, and that can be applied to membranes and lipid bilayers in aqueous media. IR spectra can be conveniently retrieved as a function of temperature, thus revealing the thermotropic transitions of SM and its mixtures with Cer. Four regions of the IR spectrum of these sphingolipids have been examined, two of them dominated by the hydrophobic regions in the molecules, namely the C-H stretching vibrations (2800-3000 cm(-1)), and the CH2 scissoring vibrations (1455-1485 cm(-1)), and two others arising from chemical groups at the lipid-water interface, the sphingolipid amide I band (1600-1680 cm(-1)), and the phosphate vibrations in the 1000-1110 cm(-1) region. The latter two regions have been rarely studied in the past. The IR data from the hydrophobic components show a gel (or ripple)-fluid transition of SM at 40 degrees C, that is shifted up to about 70 degrees C when Cer is added to the bilayers, in agreement with previous studies using a variety of techniques. IR information concerning the polar parts is more interesting. The amide I (carbonyl) band of pure SM exhibits a maximum at 1638 cm(-1) at room temperature, and its position is shifted by about 10 cm(-1) in the presence of Cer. Cer causes also a change in the overall band shape, but no signs of band splitting are seen, suggesting that SM and Cer carbonyl groups are interacting tightly, presumably through H-bonds. The 1086 cm(-1) band, corresponding to PO2- vibrations, appears more stable in SM than in DPPC, and it is further stabilized by Cer, again suggesting an important role of H-bonds in the formation of SM:Cer clusters. Thus, SM and Cer can interact through their polar headgroups, in a way that is not accessible to other lipid classes.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/50110
url http://hdl.handle.net/10810/50110
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN/PGC2018-099857-B-I00/
https://www.nature.com/articles/s41598-020-74781-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Tis article is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0)
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Tis article is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0)
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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