Functional role of native and invasive filter-feeders, and the effect of parasites: learning from hypersaline ecosystems [Dataset]
Artemia franciscana vs A. parthenogenetica. Artemia sampling was conducted during November 2012 in two different salt pan complexes located along the Atlantic coast of South West Spain. Native A. parthenogenetica was collected from Odiel saltpans (Huelva 37°15'29"N, 6°58'25"W). I...
| Autores: | , , , |
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| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/134859 |
| Acceso en línea: | http://hdl.handle.net/10261/134859 |
| Access Level: | acceso abierto |
| Palabra clave: | Artemia Cestodes Invasion Feeding Chlorophylla Filter-feeders, hypersaline ecosystems |
| Sumario: | Artemia franciscana vs A. parthenogenetica. Artemia sampling was conducted during November 2012 in two different salt pan complexes located along the Atlantic coast of South West Spain. Native A. parthenogenetica was collected from Odiel saltpans (Huelva 37°15'29"N, 6°58'25"W). Invasive A. franciscana were collected from La Tapa saltpans (Cádiz Bay 36º35’52”N, 06º13’07”W). In each locality, Artemia were collected from two separate evaporation ponds with distinct salinities (90 and 145 g/l). Artemia was sampled using a 0.5 mm mesh net, then they were immediately transported to the laboratory and transferred to plastic tanks containing aerated water from the same pond, and subjected to a natural photoperiod. Feeding rates were quantified at two salinities (90 and 145 g/l) and two temperatures (15 and 24 ºC). Prior to measuring feeding, individuals were acclimated for 12 hours in climatic chambers under experimental conditions (15ºC-90g/l, 15ºC-145g/l, 24ºC-90g/l or 24ºC-145g/l). Individuals were assigned to the experimental salinity that matched what they were exposed to in the field. We then selected 48 adult brine shrimps of similar size for each temperature-salinity treatment. We transferred them to Petri plates containing filtered (0.45 μm) and autoclaved water from the pond at the same salinity and temperature during 1h without food, in order to increase feeding motivation. To measure feeding rates, brine shrimps were placed individually into multi-well plates filled with 2.5 ml of freeze-dried green algae Tetraselmis chuii (EasyAlgae®, Spain) solution (algal concentration 0.2 mg/ml) and placed in climatic chambers at 24ºC and 15ºC. We prepared control and blank samples in triplicate for each treatment. Artemia individuals grazed during 4 hours under continuous light conditions. During this period, we gently agitated the water every 30 min with a plastic Pasteur pipette, to avoid food particle sedimentation at the bottom of the plates. At the end of the experiment we collected 1ml from each well and counted remaining algal particles using an EasyCyte Plus System flow cytometer (Guava Express Plus software). The number of consumed cells was calculated by subtracting the final number of cells from the initial number. For triplicate controls and blanks, samples of 1 ml were taken and counted before and after the experiment. Brine shrimps were anaesthetized with carbonated water before being mounted (sacrificed) in a temporary glycerol mount and examined under the microscope to confirm that no cestode parasites were present. We measured the length of each individual from the end of the abdomen (furca) to the top of the head using a stereomicroscope coupled with a videocamera (Axiovision software). Infected vs uninfected A. parthenogenetica. Artemia sampling was conducted at Odiel saltpans in spring 2013, from ponds of intermediate salinity where the prevalence of the cestodes Flamingolepis liguloides (hereafter FL), Anomotaenia tringae (AT, a shorebird parasite) and Confluaria podicipina (CP) was high (FL: pond E15 at 130g/l on 02/05/2013; AT and CP: pond E18 at 170g/l on 04/06/2013). We used the above experimental setup but with fixed temperature and salinity conditions. We conducted two independent experiments on the above dates, one for FL and another for CP-AT under similar conditions. Experiments were carried out at 130g/l salinity with a 0.2mg/l concentration of Tetraselmis chuii, calculating feeding rates as described above. Individuals collected from pond E18 were first acclimated to the experimental salinity for 12h. After 4 hours, all individuals were measured and their parasitic status confirmed as described above. Parasite identification followed Georgiev et al., 2005. For the first experiment we used 43 non-infected (hereafter NI) individuals and 55 infected with FL; for the second experiment we used 40 NI Artemia, 14 infected with CP and 27 with AT. |
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