SILAC-based nuclear proteomics uncovers antitumor mechanisms of selenium nanoparticles with in vivo validation in a melanoma model

Chitosan-stabilized selenium nanoparticles (Ch-SeNPs) are promising agents for cancer therapy due to their unique physicochemical properties, including spherical morphology and uniform size distribution. This study investigates the molecular mechanisms underlying their antitumoral effects, with a fo...

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Autores: García Calvo, Estefanía, Estévez Sánchez, Héctor, Álvarez-Fernández García, Roberto, Sánchez-Díaz, Raquel, Lazcano, Juan José, Martín, Pilar, Luque García, José Luis
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/121630
Acceso en línea:https://hdl.handle.net/20.500.14352/121630
Access Level:acceso abierto
Palabra clave:543
Química analítica (Química)
2301 Química Analítica
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spelling SILAC-based nuclear proteomics uncovers antitumor mechanisms of selenium nanoparticles with in vivo validation in a melanoma modelGarcía Calvo, EstefaníaEstévez Sánchez, HéctorÁlvarez-Fernández García, RobertoSánchez-Díaz, RaquelLazcano, Juan JoséMartín, PilarLuque García, José Luis543Química analítica (Química)2301 Química AnalíticaChitosan-stabilized selenium nanoparticles (Ch-SeNPs) are promising agents for cancer therapy due to their unique physicochemical properties, including spherical morphology and uniform size distribution. This study investigates the molecular mechanisms underlying their antitumoral effects, with a focus on the nuclear proteome. Quantitative proteomic analysis revealed 343 nuclear proteins, 47 of which showed significant changes following Ch-SeNPs treatment. Key regulators such as CDK1 and CDC5 were implicated in cell cycle arrest and tumor suppression pathways. Ch-SeNPs also affected processes including mRNA metabolism and cytoskeleton organization. In addition, Ch-SeNPs significantly inhibited tumor growth in a murine melanoma model, supporting their therapeutic potential.ElsevierUniversidad Complutense de Madrid20252025-06-0620252025-06-06journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/121630reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-114529RB-I00 NUEVOS NANOSISTEMAS MULTIFUNCIONALES PARA SU APLICACION EN BIOMEDICINA: DISEÑO, SINTESIS Y EVALUACION MEDIANTE ESTRATEGIAS (BIO)ANALITICASopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1216302026-06-02T12:44:21Z
dc.title.none.fl_str_mv SILAC-based nuclear proteomics uncovers antitumor mechanisms of selenium nanoparticles with in vivo validation in a melanoma model
title SILAC-based nuclear proteomics uncovers antitumor mechanisms of selenium nanoparticles with in vivo validation in a melanoma model
spellingShingle SILAC-based nuclear proteomics uncovers antitumor mechanisms of selenium nanoparticles with in vivo validation in a melanoma model
García Calvo, Estefanía
543
Química analítica (Química)
2301 Química Analítica
title_short SILAC-based nuclear proteomics uncovers antitumor mechanisms of selenium nanoparticles with in vivo validation in a melanoma model
title_full SILAC-based nuclear proteomics uncovers antitumor mechanisms of selenium nanoparticles with in vivo validation in a melanoma model
title_fullStr SILAC-based nuclear proteomics uncovers antitumor mechanisms of selenium nanoparticles with in vivo validation in a melanoma model
title_full_unstemmed SILAC-based nuclear proteomics uncovers antitumor mechanisms of selenium nanoparticles with in vivo validation in a melanoma model
title_sort SILAC-based nuclear proteomics uncovers antitumor mechanisms of selenium nanoparticles with in vivo validation in a melanoma model
dc.creator.none.fl_str_mv García Calvo, Estefanía
Estévez Sánchez, Héctor
Álvarez-Fernández García, Roberto
Sánchez-Díaz, Raquel
Lazcano, Juan José
Martín, Pilar
Luque García, José Luis
author García Calvo, Estefanía
author_facet García Calvo, Estefanía
Estévez Sánchez, Héctor
Álvarez-Fernández García, Roberto
Sánchez-Díaz, Raquel
Lazcano, Juan José
Martín, Pilar
Luque García, José Luis
author_role author
author2 Estévez Sánchez, Héctor
Álvarez-Fernández García, Roberto
Sánchez-Díaz, Raquel
Lazcano, Juan José
Martín, Pilar
Luque García, José Luis
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Química analítica (Química)
2301 Química Analítica
topic 543
Química analítica (Química)
2301 Química Analítica
description Chitosan-stabilized selenium nanoparticles (Ch-SeNPs) are promising agents for cancer therapy due to their unique physicochemical properties, including spherical morphology and uniform size distribution. This study investigates the molecular mechanisms underlying their antitumoral effects, with a focus on the nuclear proteome. Quantitative proteomic analysis revealed 343 nuclear proteins, 47 of which showed significant changes following Ch-SeNPs treatment. Key regulators such as CDK1 and CDC5 were implicated in cell cycle arrest and tumor suppression pathways. Ch-SeNPs also affected processes including mRNA metabolism and cytoskeleton organization. In addition, Ch-SeNPs significantly inhibited tumor growth in a murine melanoma model, supporting their therapeutic potential.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-06-06
2025
2025-06-06
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/121630
url https://hdl.handle.net/20.500.14352/121630
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-114529RB-I00 NUEVOS NANOSISTEMAS MULTIFUNCIONALES PARA SU APLICACION EN BIOMEDICINA: DISEÑO, SINTESIS Y EVALUACION MEDIANTE ESTRATEGIAS (BIO)ANALITICAS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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