Microsatellite genetic characterization of Silene ciliata Poiret (Caryophyllaceae)
We collected S. ciliata leaf tissue from 20 genets per population for genetic analysis (n = 180). DNeasy Plant minikit (QIAGEN, Valencia, USA) was used for DNA extraction of 10–20 mg of dried S. ciliata tissue. Based on a previous study (García-Fernández et al. 2012) we selected eight microsatellite...
| Autores: | , , |
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| Tipo de recurso: | conjunto de datos |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Consorcio Madroño |
| Repositorio: | e-cienciaDatos, Repositorio de Datos del Consorcio Madroño |
| OAI Identifier: | doi:10.21950/GSTZ26 |
| Acceso en línea: | https://doi.org/10.21950/GSTZ26 |
| Access Level: | acceso abierto |
| Palabra clave: | Earth and Environmental Sciences Silene ciliata genotype microsatellites population genetics |
| Sumario: | We collected S. ciliata leaf tissue from 20 genets per population for genetic analysis (n = 180). DNeasy Plant minikit (QIAGEN, Valencia, USA) was used for DNA extraction of 10–20 mg of dried S. ciliata tissue. Based on a previous study (García-Fernández et al. 2012) we selected eight microsatellite loci for genotyping: Sci1224, Sci1208, Sci0106, Sci1443, EST-2HTS, EST-37HTS, EST-G34D06 and EST-G47A02. PCR protocols were performed as described in García-Fernández et al. (2012). We genotyped all samples in an automated DNA sequencer (ABI PRISM 3730, Applied Biosystems, California, USA) in Parque Científico de Madrid (Madrid, Spain). GeneMarker version 1.85 (SoftGenetics, StateCollege, Pennsylvania, USA) was used for fragment size determination. By re-amplifying and re-scoring 20% of the samples we evaluated genotyping accuracy |
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