Transcription start site associated RNAs in bacteria
Here, we report the genome-wide identification of small RNAs associated with transcription start sites (TSSs), termed tssRNAs, in Mycoplasma pneumoniae. tssRNAs were also found to be present in a different bacterial phyla, Escherichia coli. Similar to the recently identified promoter-associated tiny...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Universitat Pompeu Fabra |
| Repositorio: | Repositorio Digital de la UPF |
| OAI Identifier: | oai:repositori.upf.edu:10230/22229 |
| Acceso en línea: | http://hdl.handle.net/10230/22229 http://dx.doi.org/10.1038/msb.2012.16 |
| Access Level: | acceso abierto |
| Palabra clave: | RNA Microbiologia |
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Transcription start site associated RNAs in bacteriaYus, EvaGüell Cargol, Marc, 1982-Vivancos Prellezo, AnaChen, Wei-HuaLluch-Senar, Maria 1982-Delgado Blanco, JavierGavin, Anne-ClaudeBork, PeerSerrano Pubull, Luis, 1982-RNAMicrobiologiaHere, we report the genome-wide identification of small RNAs associated with transcription start sites (TSSs), termed tssRNAs, in Mycoplasma pneumoniae. tssRNAs were also found to be present in a different bacterial phyla, Escherichia coli. Similar to the recently identified promoter-associated tiny RNAs (tiRNAs) in eukaryotes, tssRNAs are associated with active promoters. Evidence suggests that these tssRNAs are distinct from previously described abortive transcription RNAs. ssRNAs have an average size of 45 bases and map exactly to the beginning of cognate full-length transcripts and to cryptic TSSs. Expression of bacterial tssRNAs requires factors other than the standard RNA polymerase holoenzyme. We have found that the RNA polymerase is halted at tssRNA positions in vivo, which may indicate that a pausing mechanism exists to prevent transcription in the absence of genes. These results suggest that small RNAs associated with TSSs could be a universal feature of bacterial transcription.This work was supported by an European Research council (ERC) advanced grant, the Fundación Marcelino Botín, the Spanish Ministry of Research and ICREA (Institució Catalana de Recerca i Estudis Avançats)Nature Publishing Group201420142012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/22229http://dx.doi.org/10.1038/msb.2012.16reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésMolecular Systems Biology. 2012;8:585info:eu-repo/grantAgreement/EC/FP7/232913This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike Licensehttp://creativecommons.org/licenses/by/3.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/222292026-06-12T07:21:37Z |
| dc.title.none.fl_str_mv |
Transcription start site associated RNAs in bacteria |
| title |
Transcription start site associated RNAs in bacteria |
| spellingShingle |
Transcription start site associated RNAs in bacteria Yus, Eva RNA Microbiologia |
| title_short |
Transcription start site associated RNAs in bacteria |
| title_full |
Transcription start site associated RNAs in bacteria |
| title_fullStr |
Transcription start site associated RNAs in bacteria |
| title_full_unstemmed |
Transcription start site associated RNAs in bacteria |
| title_sort |
Transcription start site associated RNAs in bacteria |
| dc.creator.none.fl_str_mv |
Yus, Eva Güell Cargol, Marc, 1982- Vivancos Prellezo, Ana Chen, Wei-Hua Lluch-Senar, Maria 1982- Delgado Blanco, Javier Gavin, Anne-Claude Bork, Peer Serrano Pubull, Luis, 1982- |
| author |
Yus, Eva |
| author_facet |
Yus, Eva Güell Cargol, Marc, 1982- Vivancos Prellezo, Ana Chen, Wei-Hua Lluch-Senar, Maria 1982- Delgado Blanco, Javier Gavin, Anne-Claude Bork, Peer Serrano Pubull, Luis, 1982- |
| author_role |
author |
| author2 |
Güell Cargol, Marc, 1982- Vivancos Prellezo, Ana Chen, Wei-Hua Lluch-Senar, Maria 1982- Delgado Blanco, Javier Gavin, Anne-Claude Bork, Peer Serrano Pubull, Luis, 1982- |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
RNA Microbiologia |
| topic |
RNA Microbiologia |
| description |
Here, we report the genome-wide identification of small RNAs associated with transcription start sites (TSSs), termed tssRNAs, in Mycoplasma pneumoniae. tssRNAs were also found to be present in a different bacterial phyla, Escherichia coli. Similar to the recently identified promoter-associated tiny RNAs (tiRNAs) in eukaryotes, tssRNAs are associated with active promoters. Evidence suggests that these tssRNAs are distinct from previously described abortive transcription RNAs. ssRNAs have an average size of 45 bases and map exactly to the beginning of cognate full-length transcripts and to cryptic TSSs. Expression of bacterial tssRNAs requires factors other than the standard RNA polymerase holoenzyme. We have found that the RNA polymerase is halted at tssRNA positions in vivo, which may indicate that a pausing mechanism exists to prevent transcription in the absence of genes. These results suggest that small RNAs associated with TSSs could be a universal feature of bacterial transcription. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012 2014 2014 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10230/22229 http://dx.doi.org/10.1038/msb.2012.16 |
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http://hdl.handle.net/10230/22229 http://dx.doi.org/10.1038/msb.2012.16 |
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Inglés |
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Inglés |
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Molecular Systems Biology. 2012;8:585 info:eu-repo/grantAgreement/EC/FP7/232913 |
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http://creativecommons.org/licenses/by/3.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/3.0/ |
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openAccess |
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application/pdf application/pdf |
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Nature Publishing Group |
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Nature Publishing Group |
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reponame:Repositorio Digital de la UPF instname:Universitat Pompeu Fabra |
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Universitat Pompeu Fabra |
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Repositorio Digital de la UPF |
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Repositorio Digital de la UPF |
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