Time-to-positivity, type of culture media and oxidase test performed on positive blood culture vials to predict Pseudomonas aeruginosa in patients with Gram-negative bacilli bacteraemia

OBJECTIVE: The aim of this study was to determine the usefulness of oxidase test and time-to-positivity (TTP) in aerobic and anaerobic blood culture vials to detect the presence of Pseudomonas aeruginosa in patients with Gram-negative bacilli (GNB) bacteraemia. METHODS: TTP was recorded for each aer...

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Autores: Cobos-Trigueros, Nazaret, Zboromyrska, Yuliya, Morata, Laura, Alejo-Cancho, Izaskun, Calle, Cristina de la, Vergara Gómez, Andrea, Cardozo Espinola, Celia, Arcas, Maria P., Soriano Viladomiu, Alex, Marco Reverté, Francesc, Mensa Pueyo, Josep, Almela, M. (Manel), Martínez, José Antonio (Martínez Martínez)
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/109685
Acceso en línea:https://hdl.handle.net/2445/109685
Access Level:acceso abierto
Palabra clave:Pseudomonas
Infeccions nosocomials
Cultius (Biologia)
Nosocomial infections
Cultures (Biology)
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spelling Time-to-positivity, type of culture media and oxidase test performed on positive blood culture vials to predict Pseudomonas aeruginosa in patients with Gram-negative bacilli bacteraemiaCobos-Trigueros, NazaretZboromyrska, YuliyaMorata, LauraAlejo-Cancho, IzaskunCalle, Cristina de laVergara Gómez, AndreaCardozo Espinola, CeliaArcas, Maria P.Soriano Viladomiu, AlexMarco Reverté, FrancescMensa Pueyo, JosepAlmela, M. (Manel)Martínez, José Antonio (Martínez Martínez)PseudomonasInfeccions nosocomialsCultius (Biologia)PseudomonasNosocomial infectionsCultures (Biology)OBJECTIVE: The aim of this study was to determine the usefulness of oxidase test and time-to-positivity (TTP) in aerobic and anaerobic blood culture vials to detect the presence of Pseudomonas aeruginosa in patients with Gram-negative bacilli (GNB) bacteraemia. METHODS: TTP was recorded for each aerobic and anaerobic blood culture vial of monomicrobial bacteraemia due to GNB. Oxidase test was performed in a pellet of the centrifuged content of the positive blood culture. An algorithm was developed in order to perform the oxidase test efficiently taking into account TTP and type of vial. RESULTS: A total of 341 episodes of GNB bacteraemia were analysed. Sensitivity, specificity, positive predictive value and negative predictive value of the oxidase test performed on positive vials with GNB to predict P. aeruginosa were 95%, 99%, 91%, and 99%, respectively. When growth was first or exclusively detected in anaerobic vials, P. aeruginosa was never identified hence the performance of the oxidase test could be avoided. When growth was only or first detected in aerobic vials, a TTP≥8h predicted P. aeruginosa in 37% or cases (63 of 169), therefore oxidase test is highly recommended. CONCLUSIONS: Oxidase test performed onto positive blood culture vials previously selected by TTP and type of vials is an easy and inexpensive way to predict P. aeruginosa. In most cases, this can lead to optimization of treatment in less than 24 hours.Sociedad Española de Quimioterapia2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/109685Articles publicats en revistes (Medicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a:Revista Espanola de Quimioterapia, 2017, vol. 30, num. 1, p. 9-13(c) Sociedad Española de Quimioterapia, 2017info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1096852026-05-27T06:46:51Z
dc.title.none.fl_str_mv Time-to-positivity, type of culture media and oxidase test performed on positive blood culture vials to predict Pseudomonas aeruginosa in patients with Gram-negative bacilli bacteraemia
title Time-to-positivity, type of culture media and oxidase test performed on positive blood culture vials to predict Pseudomonas aeruginosa in patients with Gram-negative bacilli bacteraemia
spellingShingle Time-to-positivity, type of culture media and oxidase test performed on positive blood culture vials to predict Pseudomonas aeruginosa in patients with Gram-negative bacilli bacteraemia
Cobos-Trigueros, Nazaret
Pseudomonas
Infeccions nosocomials
Cultius (Biologia)
Pseudomonas
Nosocomial infections
Cultures (Biology)
title_short Time-to-positivity, type of culture media and oxidase test performed on positive blood culture vials to predict Pseudomonas aeruginosa in patients with Gram-negative bacilli bacteraemia
title_full Time-to-positivity, type of culture media and oxidase test performed on positive blood culture vials to predict Pseudomonas aeruginosa in patients with Gram-negative bacilli bacteraemia
title_fullStr Time-to-positivity, type of culture media and oxidase test performed on positive blood culture vials to predict Pseudomonas aeruginosa in patients with Gram-negative bacilli bacteraemia
title_full_unstemmed Time-to-positivity, type of culture media and oxidase test performed on positive blood culture vials to predict Pseudomonas aeruginosa in patients with Gram-negative bacilli bacteraemia
title_sort Time-to-positivity, type of culture media and oxidase test performed on positive blood culture vials to predict Pseudomonas aeruginosa in patients with Gram-negative bacilli bacteraemia
dc.creator.none.fl_str_mv Cobos-Trigueros, Nazaret
Zboromyrska, Yuliya
Morata, Laura
Alejo-Cancho, Izaskun
Calle, Cristina de la
Vergara Gómez, Andrea
Cardozo Espinola, Celia
Arcas, Maria P.
Soriano Viladomiu, Alex
Marco Reverté, Francesc
Mensa Pueyo, Josep
Almela, M. (Manel)
Martínez, José Antonio (Martínez Martínez)
author Cobos-Trigueros, Nazaret
author_facet Cobos-Trigueros, Nazaret
Zboromyrska, Yuliya
Morata, Laura
Alejo-Cancho, Izaskun
Calle, Cristina de la
Vergara Gómez, Andrea
Cardozo Espinola, Celia
Arcas, Maria P.
Soriano Viladomiu, Alex
Marco Reverté, Francesc
Mensa Pueyo, Josep
Almela, M. (Manel)
Martínez, José Antonio (Martínez Martínez)
author_role author
author2 Zboromyrska, Yuliya
Morata, Laura
Alejo-Cancho, Izaskun
Calle, Cristina de la
Vergara Gómez, Andrea
Cardozo Espinola, Celia
Arcas, Maria P.
Soriano Viladomiu, Alex
Marco Reverté, Francesc
Mensa Pueyo, Josep
Almela, M. (Manel)
Martínez, José Antonio (Martínez Martínez)
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pseudomonas
Infeccions nosocomials
Cultius (Biologia)
Pseudomonas
Nosocomial infections
Cultures (Biology)
topic Pseudomonas
Infeccions nosocomials
Cultius (Biologia)
Pseudomonas
Nosocomial infections
Cultures (Biology)
description OBJECTIVE: The aim of this study was to determine the usefulness of oxidase test and time-to-positivity (TTP) in aerobic and anaerobic blood culture vials to detect the presence of Pseudomonas aeruginosa in patients with Gram-negative bacilli (GNB) bacteraemia. METHODS: TTP was recorded for each aerobic and anaerobic blood culture vial of monomicrobial bacteraemia due to GNB. Oxidase test was performed in a pellet of the centrifuged content of the positive blood culture. An algorithm was developed in order to perform the oxidase test efficiently taking into account TTP and type of vial. RESULTS: A total of 341 episodes of GNB bacteraemia were analysed. Sensitivity, specificity, positive predictive value and negative predictive value of the oxidase test performed on positive vials with GNB to predict P. aeruginosa were 95%, 99%, 91%, and 99%, respectively. When growth was first or exclusively detected in anaerobic vials, P. aeruginosa was never identified hence the performance of the oxidase test could be avoided. When growth was only or first detected in aerobic vials, a TTP≥8h predicted P. aeruginosa in 37% or cases (63 of 169), therefore oxidase test is highly recommended. CONCLUSIONS: Oxidase test performed onto positive blood culture vials previously selected by TTP and type of vials is an easy and inexpensive way to predict P. aeruginosa. In most cases, this can lead to optimization of treatment in less than 24 hours.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/109685
url https://hdl.handle.net/2445/109685
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a:
Revista Espanola de Quimioterapia, 2017, vol. 30, num. 1, p. 9-13
dc.rights.none.fl_str_mv (c) Sociedad Española de Quimioterapia, 2017
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Sociedad Española de Quimioterapia, 2017
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedad Española de Quimioterapia
publisher.none.fl_str_mv Sociedad Española de Quimioterapia
dc.source.none.fl_str_mv Articles publicats en revistes (Medicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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