Assessing neurological and cardiovascular effects caused by pharmaceuticals in river water: Insights from Daphnia magna and Danio rerio embryos

Pharmaceutical residues in surface waters are an emerging environmental and public health issue, yet their biological impacts on aquatic life remain poorly understood. This study presents a cost-effective bioanalytical framework using Daphnia magna juveniles and zebrafish (Danio rerio) embryos to ev...

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Detalles Bibliográficos
Autores: Romero Alfano, Irene, Prats, Eva, Raldua, Demetrio, Blazquez, Mercedes, Tauler, Roma, Piña, Benjamí, Gómez-Canela, Cristian, Barata, Carlos
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.14342/5390
Acceso en línea:http://hdl.handle.net/20.500.14342/5390
https://doi.org/10.1016/j.chemosphere.2025.144541
Access Level:acceso abierto
Palabra clave:Pharmaceuticals
Neurotoxic
Daphnia magna
Zebrafish
Wastewater
Environmental risk assessment
Indústria farmacèutica
Neurotoxines
Clàdocers
Peix zebra
Aigua--Contaminació
Medi ambient--Avaluació del risc
502
615
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spelling Assessing neurological and cardiovascular effects caused by pharmaceuticals in river water: Insights from Daphnia magna and Danio rerio embryosRomero Alfano, IrenePrats, EvaRaldua, DemetrioBlazquez, MercedesTauler, RomaPiña, BenjamíGómez-Canela, CristianBarata, CarlosPharmaceuticalsNeurotoxicDaphnia magnaZebrafishWastewaterEnvironmental risk assessmentIndústria farmacèuticaNeurotoxinesClàdocersPeix zebraAigua--ContaminacióMedi ambient--Avaluació del risc502615Pharmaceutical residues in surface waters are an emerging environmental and public health issue, yet their biological impacts on aquatic life remain poorly understood. This study presents a cost-effective bioanalytical framework using Daphnia magna juveniles and zebrafish (Danio rerio) embryos to evaluate neurotoxic and cardiotoxic effects of pharmaceutical mixtures in rivers downstream of wastewater treatment plant (WWTP) effluents. Water samples from three rivers in north-eastern Spain (Besòs, Llobregat, and Onyar) were concentrated up to 5- and 20-fold using solid-phase extraction. Bioassays were conducted over 24 h for D. magna and five days for zebrafish embryos. Eighty pharmaceutical compounds were quantified via HPLC-MS and linked with phenotypic endpoints including locomotion, feeding, heart rate, neurotransmitter profiles, and metabolomic alterations. Of the 28 concentrated extracts, four were acutely toxic to zebrafish embryos. Altered behavioral and cardiovascular responses were observed in 22.2 % (D. magna) and 35.1 % (D. rerio) of extracts, primarily at higher enrichment. Concentrations of 31 pharmaceuticals were statistically associated with observed effects. Neuroactive drugs such as topiramate, rasagiline, citalopram, and fluvoxamine showed strong correlations with altered neurotransmitter levels in zebrafish, consistent with their known mechanisms. Seven additional compounds with secondary neuroactive properties were linked to similar neurological disruptions. Seventeen pharmaceuticals were associated with disturbances in amino acid metabolism and urea cycle pathways, indicating broader metabolic dysregulation. Overall, nearly 75 % of river extracts showed no observable effect, but several samples were acutely toxic or induced sublethal neurobehavioral and metabolic responses. These findings support the utility of D. magna and zebrafish (D. rerio) embryos as sensitive and complementary biosentinels for monitoring pharmaceutical pollution and highlight zebrafish as a relevant model for studying environmentally driven neurotoxicity with potential human health implications.info:eu-repo/semantics/publishedVersionElsevierUniversitat Ramon Llull. IQS2025info:eu-repo/semantics/articlep.13http://hdl.handle.net/20.500.14342/5390https://doi.org/10.1016/j.chemosphere.2025.144541reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésChemosphere 2025, 385info:eu-repo/grantAgreement/AEI-MCIN/PN I+D/TED2021-130845A-C32info:eu-repo/grantAgreement/AEI-MCIN/PN I+D/TED2021-130845B–C31info:eu-repo/grantAgreement/MCIU/PN I+D/PID2023–148502OB-C21info:eu-repo/grantAgreement/MCIU/PN I+D/PID2023–148502OB-C22info:eu-repo/grantAgreement/SUR del DEC/SGR/236SGR21© L'autor/aAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.14342/53902026-05-29T05:05:01Z
dc.title.none.fl_str_mv Assessing neurological and cardiovascular effects caused by pharmaceuticals in river water: Insights from Daphnia magna and Danio rerio embryos
title Assessing neurological and cardiovascular effects caused by pharmaceuticals in river water: Insights from Daphnia magna and Danio rerio embryos
spellingShingle Assessing neurological and cardiovascular effects caused by pharmaceuticals in river water: Insights from Daphnia magna and Danio rerio embryos
Romero Alfano, Irene
Pharmaceuticals
Neurotoxic
Daphnia magna
Zebrafish
Wastewater
Environmental risk assessment
Indústria farmacèutica
Neurotoxines
Clàdocers
Peix zebra
Aigua--Contaminació
Medi ambient--Avaluació del risc
502
615
title_short Assessing neurological and cardiovascular effects caused by pharmaceuticals in river water: Insights from Daphnia magna and Danio rerio embryos
title_full Assessing neurological and cardiovascular effects caused by pharmaceuticals in river water: Insights from Daphnia magna and Danio rerio embryos
title_fullStr Assessing neurological and cardiovascular effects caused by pharmaceuticals in river water: Insights from Daphnia magna and Danio rerio embryos
title_full_unstemmed Assessing neurological and cardiovascular effects caused by pharmaceuticals in river water: Insights from Daphnia magna and Danio rerio embryos
title_sort Assessing neurological and cardiovascular effects caused by pharmaceuticals in river water: Insights from Daphnia magna and Danio rerio embryos
dc.creator.none.fl_str_mv Romero Alfano, Irene
Prats, Eva
Raldua, Demetrio
Blazquez, Mercedes
Tauler, Roma
Piña, Benjamí
Gómez-Canela, Cristian
Barata, Carlos
author Romero Alfano, Irene
author_facet Romero Alfano, Irene
Prats, Eva
Raldua, Demetrio
Blazquez, Mercedes
Tauler, Roma
Piña, Benjamí
Gómez-Canela, Cristian
Barata, Carlos
author_role author
author2 Prats, Eva
Raldua, Demetrio
Blazquez, Mercedes
Tauler, Roma
Piña, Benjamí
Gómez-Canela, Cristian
Barata, Carlos
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Ramon Llull. IQS
dc.subject.none.fl_str_mv Pharmaceuticals
Neurotoxic
Daphnia magna
Zebrafish
Wastewater
Environmental risk assessment
Indústria farmacèutica
Neurotoxines
Clàdocers
Peix zebra
Aigua--Contaminació
Medi ambient--Avaluació del risc
502
615
topic Pharmaceuticals
Neurotoxic
Daphnia magna
Zebrafish
Wastewater
Environmental risk assessment
Indústria farmacèutica
Neurotoxines
Clàdocers
Peix zebra
Aigua--Contaminació
Medi ambient--Avaluació del risc
502
615
description Pharmaceutical residues in surface waters are an emerging environmental and public health issue, yet their biological impacts on aquatic life remain poorly understood. This study presents a cost-effective bioanalytical framework using Daphnia magna juveniles and zebrafish (Danio rerio) embryos to evaluate neurotoxic and cardiotoxic effects of pharmaceutical mixtures in rivers downstream of wastewater treatment plant (WWTP) effluents. Water samples from three rivers in north-eastern Spain (Besòs, Llobregat, and Onyar) were concentrated up to 5- and 20-fold using solid-phase extraction. Bioassays were conducted over 24 h for D. magna and five days for zebrafish embryos. Eighty pharmaceutical compounds were quantified via HPLC-MS and linked with phenotypic endpoints including locomotion, feeding, heart rate, neurotransmitter profiles, and metabolomic alterations. Of the 28 concentrated extracts, four were acutely toxic to zebrafish embryos. Altered behavioral and cardiovascular responses were observed in 22.2 % (D. magna) and 35.1 % (D. rerio) of extracts, primarily at higher enrichment. Concentrations of 31 pharmaceuticals were statistically associated with observed effects. Neuroactive drugs such as topiramate, rasagiline, citalopram, and fluvoxamine showed strong correlations with altered neurotransmitter levels in zebrafish, consistent with their known mechanisms. Seven additional compounds with secondary neuroactive properties were linked to similar neurological disruptions. Seventeen pharmaceuticals were associated with disturbances in amino acid metabolism and urea cycle pathways, indicating broader metabolic dysregulation. Overall, nearly 75 % of river extracts showed no observable effect, but several samples were acutely toxic or induced sublethal neurobehavioral and metabolic responses. These findings support the utility of D. magna and zebrafish (D. rerio) embryos as sensitive and complementary biosentinels for monitoring pharmaceutical pollution and highlight zebrafish as a relevant model for studying environmentally driven neurotoxicity with potential human health implications.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.14342/5390
https://doi.org/10.1016/j.chemosphere.2025.144541
url http://hdl.handle.net/20.500.14342/5390
https://doi.org/10.1016/j.chemosphere.2025.144541
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Chemosphere 2025, 385
info:eu-repo/grantAgreement/AEI-MCIN/PN I+D/TED2021-130845A-C32
info:eu-repo/grantAgreement/AEI-MCIN/PN I+D/TED2021-130845B–C31
info:eu-repo/grantAgreement/MCIU/PN I+D/PID2023–148502OB-C21
info:eu-repo/grantAgreement/MCIU/PN I+D/PID2023–148502OB-C22
info:eu-repo/grantAgreement/SUR del DEC/SGR/236SGR21
dc.rights.none.fl_str_mv © L'autor/a
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © L'autor/a
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv p.13
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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