Xenopus HJURP and condensin II are required for CENP-A assembly.

Centromeric protein A (CENP-A) is the epigenetic mark of centromeres. CENP-A replenishment is necessary in each cell cycle to compensate for the dilution associated to DNA replication, but how this is achieved mechanistically is largely unknown. We have developed an assay using Xenopus egg extracts...

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Detalles Bibliográficos
Autores: Bernad, Rafael, Sánchez, Patricia, Rivera, Teresa, Rodríguez-Corsino, Miriam, Boyarchuk, Ekaterina, Vassias, Isabelle, Ray-Gallet, Dominique, Arnaoutov, Alexei, Dasso, Mary, Almouzni, Geneviève, Losada, Ana
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/17952
Acceso en línea:http://hdl.handle.net/20.500.12105/17952
Access Level:acceso abierto
Palabra clave:Adenosine Triphosphatases
Animals
Autoantigens
Centromere
Centromere Protein A
Chromatin
Chromosomal Proteins, Non-Histone
DNA-Binding Proteins
Epigenesis, Genetic
Histone Chaperones
Humans
Interphase
Multiprotein Complexes
Xenopus Proteins
Xenopus laevis
Descripción
Sumario:Centromeric protein A (CENP-A) is the epigenetic mark of centromeres. CENP-A replenishment is necessary in each cell cycle to compensate for the dilution associated to DNA replication, but how this is achieved mechanistically is largely unknown. We have developed an assay using Xenopus egg extracts that can recapitulate the spatial and temporal specificity of CENP-A deposition observed in human cells, providing us with a robust in vitro system amenable to molecular dissection. Here we show that this deposition depends on Xenopus Holliday junction-recognizing protein (xHJURP), a member of the HJURP/Scm3 family recently identified in yeast and human cells, further supporting the essential role of these chaperones in CENP-A loading. Despite little sequence homology, human HJURP can substitute for xHJURP. We also report that condensin II, but not condensin I, is required for CENP-A assembly and contributes to retention of centromeric CENP-A nucleosomes both in mitosis and interphase. We propose that the chromatin structure imposed by condensin II at centromeres enables CENP-A incorporation initiated by xHJURP.