Bicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamers

Producción Científica

Detalles Bibliográficos
Autores: Cipriani, Filippo, Dominik Bernhagen, García Arévalo, María del Carmen, González de Torre, Israel, Timmerman, Peter, Rodríguez Cabello, José Carlos
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/34880
Acceso en línea:https://doi.org/10.1088/1748-605X/aafd83
http://uvadoc.uva.es/handle/10324/34880
Access Level:acceso abierto
Palabra clave:Medicina regenerativa
Regenerative medicine
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spelling Bicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamersCipriani, FilippoDominik BernhagenGarcía Arévalo, María del CarmenGonzález de Torre, IsraelTimmerman, PeterRodríguez Cabello, José CarlosMedicina regenerativaRegenerative medicineProducción CientíficaBiomaterial design in tissue engineering aims to identify appropriate cellular microenvironments in which cells can grow and guide new tissue formation. Despite the large diversity of synthetic polymers available for regenerative medicine, most of them fail to fully match the functional properties of their native counterparts. In contrast, the few biological alternatives employed as biomaterials lack the versatility that chemical synthesis can offer. Herein, we studied the HUVEC adhesion and proliferation properties of elastin-like recombinamers (ELRs) that were covalently functionalized with each three high-affinity and selectivity αvβ3- and α5β1-binding bicyclic RGD peptides. Next to the bicycles, ELRs were also functionalized with various integrin-binding benchmark peptides, i.e. knottin-RGD, cyclo-[KRGDf] and GRGDS, allowing for better classification of the obtained results. Covalent functionalization with the RGD peptides, as validated by MALDI-TOF analysis, guarantees flexibility and minimal steric hindrance for interactions with cellular integrins. In addition to the covalently modified RGD-ELRs, we also synthesized another benchmark ELR comprising RGD as part of the backbone. HUVEC adhesion and proliferation analysis using the PicoGreen® assay revealed a higher short-term adhesion and proliferative capacity of cells on ELR surfaces functionalized with high affinity, integrin-binding bicyclic RGD-peptides compared with the ELRs containing RGD in the backbone.2020-01-202020-01-20European Commission (NMP-2014-646075, MSCA-ITN-2014-ETN-642687)Ministerio de Economía, Industria y Competitividad (Projects PCIN-2015-010, 26 MAT2015-68901-R, MAT2016-78903-R)Junta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. VA015U16)IOP Publishing2019info:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1088/1748-605X/aafd83http://uvadoc.uva.es/handle/10324/34880reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://iopscience.iop.org/article/10.1088/1748-605X/aafd83info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:uvadoc.uva.es:10324/348802026-06-13T12:44:47Z
dc.title.none.fl_str_mv Bicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamers
title Bicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamers
spellingShingle Bicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamers
Cipriani, Filippo
Medicina regenerativa
Regenerative medicine
title_short Bicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamers
title_full Bicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamers
title_fullStr Bicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamers
title_full_unstemmed Bicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamers
title_sort Bicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamers
dc.creator.none.fl_str_mv Cipriani, Filippo
Dominik Bernhagen
García Arévalo, María del Carmen
González de Torre, Israel
Timmerman, Peter
Rodríguez Cabello, José Carlos
author Cipriani, Filippo
author_facet Cipriani, Filippo
Dominik Bernhagen
García Arévalo, María del Carmen
González de Torre, Israel
Timmerman, Peter
Rodríguez Cabello, José Carlos
author_role author
author2 Dominik Bernhagen
García Arévalo, María del Carmen
González de Torre, Israel
Timmerman, Peter
Rodríguez Cabello, José Carlos
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Medicina regenerativa
Regenerative medicine
topic Medicina regenerativa
Regenerative medicine
description Producción Científica
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://doi.org/10.1088/1748-605X/aafd83
http://uvadoc.uva.es/handle/10324/34880
url https://doi.org/10.1088/1748-605X/aafd83
http://uvadoc.uva.es/handle/10324/34880
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://iopscience.iop.org/article/10.1088/1748-605X/aafd83
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv IOP Publishing
publisher.none.fl_str_mv IOP Publishing
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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