Phenotyping and susceptibility of established porcine cells lines to African Swine Fever Virus infection and viral production

African swine fever virus (ASFV) is a highly pathogenic, double-stranded DNA virus with a marked tropism for cells of the monocyte-macrophage lineage, affecting swine species and provoking severe economic losses and health threats. In the present study, four established porcine cell lines, IPAM-WT,...

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Bibliographic Details
Authors: Sánchez, Elena G., Riera, Elena, Nogal, Marisa, Gallardo, Carmina, Revilla, Yolanda, Fernández Freire, Paloma, Bello-Morales Arroyo, Ángeles Raquel, López Guerrero, José Antonio, Chitko-Mckown, Carol G., Richt, Jürgen A.
Format: article
Publication Date:2017
Country:España
Institution:Universidad Autónoma de Madrid
Repository:Biblos-e Archivo. Repositorio Institucional de la UAM
Language:English
OAI Identifier:oai:repositorio.uam.es:10486/715511
Online Access:http://hdl.handle.net/10486/715511
https://dx.doi.org/10.1038/s41598-017-09948-x
Access Level:Open access
Keyword:African swine fever virus (ASFV)
porcine cell lines
Biología y Biomedicina / Biología
Description
Summary:African swine fever virus (ASFV) is a highly pathogenic, double-stranded DNA virus with a marked tropism for cells of the monocyte-macrophage lineage, affecting swine species and provoking severe economic losses and health threats. In the present study, four established porcine cell lines, IPAM-WT, IPAM-CD163, Câ2+ and WSL, were compared to porcine alveolar macrophage (PAM) in terms of surface marker phenotype, susceptibility to ASFV infection and virus production. The virulent ASFV Armenia/07, E70 or the naturally attenuated NHV/P68 strains were used as viral models. Cells expressed only low levels of specific receptors linked to the monocyte/macrophage lineage, with low levels of infection overall, with the exception of WSL, which showed more efficient production of strain NHV/P68 but not of strains E70 and Armenia/07

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