Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus)

5 páginas, 3 tablas, 2 figuras

Detalles Bibliográficos
Autores: Velasco, Amaya, Sánchez, Ana Cristina, Martínez, Iciar, Santaclara, Francisco J., Pérez Martín, Ricardo Isaac, González Sotelo, Carmen
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/78850
Acceso en línea:http://hdl.handle.net/10261/78850
Access Level:acceso abierto
Palabra clave:Atlantic mackerel
Scomber
Species identification
Pelagic fish
Real-Time PCR
Cytochrome b
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spelling Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus)Velasco, AmayaSánchez, Ana CristinaMartínez, IciarSantaclara, Francisco J.Pérez Martín, Ricardo IsaacGonzález Sotelo, CarmenAtlantic mackerelScomberSpecies identificationPelagic fishReal-Time PCRCytochrome b5 páginas, 3 tablas, 2 figurasA Real Time-PCR method based on TaqMan technology for the identification of Scomber scombrus has been developed. A system of specific primers and a Minor Groove Binding (MGB) TaqMan probe based on sequences of the mitochondrial cytochrome b region was designed. The method was successfully tested in 81 specimens of S. scombrus and related species and validated in 26 different commercial samples. An average Threshold Cycle (Ct) value of 15.3 was obtained with S. scombrus DNA. With the other species tested fluorescence signal was not detected or Ct was significantly higher (P < 0.001). The efficiency of the assay was estimated to be 92.41%, with 100% specificity, and no cross reactivity was detected with any other species. These results reveal that the developed method is a rapid and efficient tool to unequivocally identify S. scombrus and may aid in the prevention of fraud or mislabelling in mackerel productsThe work was supported by the project “Traceability control mechanisms in the mackerel food chain between Norway and Japan – TraCtrolMac” (No: 251810704011000009)Peer reviewedElsevier201320132013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/78850reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.foodchem.2013.05.077info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/788502026-05-22T06:33:51Z
dc.title.none.fl_str_mv Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus)
title Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus)
spellingShingle Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus)
Velasco, Amaya
Atlantic mackerel
Scomber
Species identification
Pelagic fish
Real-Time PCR
Cytochrome b
title_short Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus)
title_full Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus)
title_fullStr Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus)
title_full_unstemmed Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus)
title_sort Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus)
dc.creator.none.fl_str_mv Velasco, Amaya
Sánchez, Ana Cristina
Martínez, Iciar
Santaclara, Francisco J.
Pérez Martín, Ricardo Isaac
González Sotelo, Carmen
author Velasco, Amaya
author_facet Velasco, Amaya
Sánchez, Ana Cristina
Martínez, Iciar
Santaclara, Francisco J.
Pérez Martín, Ricardo Isaac
González Sotelo, Carmen
author_role author
author2 Sánchez, Ana Cristina
Martínez, Iciar
Santaclara, Francisco J.
Pérez Martín, Ricardo Isaac
González Sotelo, Carmen
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Atlantic mackerel
Scomber
Species identification
Pelagic fish
Real-Time PCR
Cytochrome b
topic Atlantic mackerel
Scomber
Species identification
Pelagic fish
Real-Time PCR
Cytochrome b
description 5 páginas, 3 tablas, 2 figuras
publishDate 2013
dc.date.none.fl_str_mv 2013
2013
2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/78850
url http://hdl.handle.net/10261/78850
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.foodchem.2013.05.077
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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