Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients

Brain corpora amylacea, recently renamed as wasteosomes, are polyglucosan bodies that appear during aging and some neurodegenerative conditions. They collect waste substances and are part of a brain cleaning mechanism. For decades, studies on their composition have produced inconsistent results and...

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Autores: Riba Baqués, Marta, Valle i Macià, Jaume del, Romera, Clara, Alsina Planelles, Raquel, Molina Porcel, Laura, Pelegrí i Gabaldà, Carme, Vilaplana i Hortensi, Jordi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/199042
Acceso en línea:https://hdl.handle.net/2445/199042
Access Level:acceso abierto
Palabra clave:Envelliment cerebral
Hipocamp (Cervell)
Malaltia d'Alzheimer
Aging brain
Hippocampus (Brain)
Alzheimer's disease
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spelling Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patientsRiba Baqués, MartaValle i Macià, Jaume delRomera, ClaraAlsina Planelles, RaquelMolina Porcel, LauraPelegrí i Gabaldà, CarmeVilaplana i Hortensi, JordiEnvelliment cerebralHipocamp (Cervell)Malaltia d'AlzheimerAging brainHippocampus (Brain)Alzheimer's diseaseBrain corpora amylacea, recently renamed as wasteosomes, are polyglucosan bodies that appear during aging and some neurodegenerative conditions. They collect waste substances and are part of a brain cleaning mechanism. For decades, studies on their composition have produced inconsistent results and the presence of tau protein in them has been controversial. In this work, we reanalyzed the presence of this protein in wasteosomes and we pointed out a methodological problem when immunolabeling. It is well known that to detect tau it is necessary to perform an antigen retrieval. However, in the case of wasteosomes, an excessive antigen retrieval with boiling dissolves their polyglucosan structure, releases the entrapped proteins and, thus, prevents their detection. After performing an adequate pre-treatment, with an intermediate time of boiling, we observed that some brain wasteosomes from patients with Alzheimer's disease (AD) contained tau, while we did not detect tau protein in those from non-AD patients. These observations pointed the different composition of wasteosomes depending on the neuropathological condition and reinforce the role of wasteosomes as waste containers.Frontiers Media2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/199042Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fnagi.2023.1110425Frontiers in Aging Neuroscience, 2023, vol. 15, p. 1110425https://doi.org/10.3389/fnagi.2023.1110425cc-by (c) Riba Baques, Marta et al., 2023https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1990422026-05-27T06:46:51Z
dc.title.none.fl_str_mv Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients
title Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients
spellingShingle Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients
Riba Baqués, Marta
Envelliment cerebral
Hipocamp (Cervell)
Malaltia d'Alzheimer
Aging brain
Hippocampus (Brain)
Alzheimer's disease
title_short Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients
title_full Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients
title_fullStr Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients
title_full_unstemmed Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients
title_sort Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients
dc.creator.none.fl_str_mv Riba Baqués, Marta
Valle i Macià, Jaume del
Romera, Clara
Alsina Planelles, Raquel
Molina Porcel, Laura
Pelegrí i Gabaldà, Carme
Vilaplana i Hortensi, Jordi
author Riba Baqués, Marta
author_facet Riba Baqués, Marta
Valle i Macià, Jaume del
Romera, Clara
Alsina Planelles, Raquel
Molina Porcel, Laura
Pelegrí i Gabaldà, Carme
Vilaplana i Hortensi, Jordi
author_role author
author2 Valle i Macià, Jaume del
Romera, Clara
Alsina Planelles, Raquel
Molina Porcel, Laura
Pelegrí i Gabaldà, Carme
Vilaplana i Hortensi, Jordi
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Envelliment cerebral
Hipocamp (Cervell)
Malaltia d'Alzheimer
Aging brain
Hippocampus (Brain)
Alzheimer's disease
topic Envelliment cerebral
Hipocamp (Cervell)
Malaltia d'Alzheimer
Aging brain
Hippocampus (Brain)
Alzheimer's disease
description Brain corpora amylacea, recently renamed as wasteosomes, are polyglucosan bodies that appear during aging and some neurodegenerative conditions. They collect waste substances and are part of a brain cleaning mechanism. For decades, studies on their composition have produced inconsistent results and the presence of tau protein in them has been controversial. In this work, we reanalyzed the presence of this protein in wasteosomes and we pointed out a methodological problem when immunolabeling. It is well known that to detect tau it is necessary to perform an antigen retrieval. However, in the case of wasteosomes, an excessive antigen retrieval with boiling dissolves their polyglucosan structure, releases the entrapped proteins and, thus, prevents their detection. After performing an adequate pre-treatment, with an intermediate time of boiling, we observed that some brain wasteosomes from patients with Alzheimer's disease (AD) contained tau, while we did not detect tau protein in those from non-AD patients. These observations pointed the different composition of wasteosomes depending on the neuropathological condition and reinforce the role of wasteosomes as waste containers.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/199042
url https://hdl.handle.net/2445/199042
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fnagi.2023.1110425
Frontiers in Aging Neuroscience, 2023, vol. 15, p. 1110425
https://doi.org/10.3389/fnagi.2023.1110425
dc.rights.none.fl_str_mv cc-by (c) Riba Baques, Marta et al., 2023
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Riba Baques, Marta et al., 2023
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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