Virulotyping of Salmonella Enterica Serovar Typhi Isolates from Pakistan: Absence of Complete SPI-10 in Vi Negative Isolates

The pathogenesis of Salmonella enterica serovar Typhi (S. Typhi), the cause of typhoid fever in humans, is mainly attributed to the acquisition of horizontally acquired DNA elements. Salmonella pathogenicity islands (SPIs) are indubitably the most important form of horizontally acquired DNA with res...

ver descrição completa

Detalhes bibliográficos
Autores: Liaquat, Sadia, Sarwar, Yasra, Ali, Aamir, Haque, Abdul, Farooq, Muhammad, Martínez Ballesteros, Ilargi, Laorden Muñoz, Lorena, Garaizar Candina, Javier, Bikandi Bikandi, Joseba
Formato: artículo
Fecha de publicación:2018
País:España
Recursos:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/30885
Acesso em linha:http://hdl.handle.net/10810/30885
Access Level:acceso abierto
Palavra-chave:pathogenicity islands
variable-number
tandem repeats
gene sequence
multiplex pcr
antigen
fever
typhimurium
expression
id ES_b4fd2f0e07fd61382e4bf8a0318b4486
oai_identifier_str oai:addi.ehu.eus:10810/30885
network_acronym_str ES
network_name_str España
repository_id_str
spelling Virulotyping of Salmonella Enterica Serovar Typhi Isolates from Pakistan: Absence of Complete SPI-10 in Vi Negative IsolatesLiaquat, SadiaSarwar, YasraAli, AamirHaque, AbdulFarooq, MuhammadMartínez Ballesteros, IlargiLaorden Muñoz, LorenaGaraizar Candina, JavierBikandi Bikandi, Josebapathogenicity islandsvariable-numbertandem repeatsgene sequencemultiplex pcrantigenfevertyphimuriumexpressionThe pathogenesis of Salmonella enterica serovar Typhi (S. Typhi), the cause of typhoid fever in humans, is mainly attributed to the acquisition of horizontally acquired DNA elements. Salmonella pathogenicity islands (SPIs) are indubitably the most important form of horizontally acquired DNA with respect to pathogenesis of this bacterium. The insertion or deletion of any of these transferrable SPIs may have impact on the virulence potential of S. Typhi. In this study, the virulence potential and genetic relatedness of 35 S. Typhi isolates, collected from 2004 to 2013 was determined by identification of SPI and non-SPI virulence factors through a combination of techniques including virulotyping, Whole Genome Sequencing (WGS), and Variable Number of Tandem Repeats (VNTR) profiling. In order to determine the virulence potential of local S. Typhi isolates, 56 virulence related genes were studied by PCR. These genes are located in the core as well as accessory genome (SPIs and plasmid). Major variations among studied virulence determinants were found in case of SPI-7 and SPI-10 associated genes. On the basis of presence of virulence related genes, the studied S. Typhi isolates from Pakistan were clustered into two virulotypes Vi-positive and Vi-negative. Interestingly, SPI-7 and SPI-10 were collectively absent or present in Vinegative and Vi-positive strains, respectively. Two Vi-negative and 11 Vi-positive S. Typhi strains were also analyzed by whole genome sequencing (WGS) and their results supported the PCR results. Genetic diversity was tested by VNTR-based molecular typing. All 35 isolates were clustered into five groups. Overall, all Vi-negative isolates were placed in a single group (T5) whereas Vi-positive isolates were grouped into four types. Vi-negative and Vi-positive isolates were mutually exclusive. This is the first report on the comparative distribution of SPI and non-SPI related virulence genes in Vi-negative and Vi-positive S. Typhi isolates with an important finding that SPI-10 is absent in all Vi-negative isolates.The Whole Genome Sequencing (WGS) was funded by the Consolider-Ingenio 2010 program of the Spanish Government (CDS 2009-00006), and Innuendo project co-funded by the European Food Safety Agency (EFSA) (GP/EFSA/AFSCO/2015/01/CT2). The rest of the research work was funded by Higher Education Commission of Pakistan (HEC) and Vital Foundation of Vitoria-Gastiez. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library Science201920192018info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/30885reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0006839info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/Copyright: © 2018 Liaquat et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Attribution 4.0 International (CC BY 4.0)Atribución 3.0 Españaoai:addi.ehu.eus:10810/308852026-06-18T09:23:17Z
dc.title.none.fl_str_mv Virulotyping of Salmonella Enterica Serovar Typhi Isolates from Pakistan: Absence of Complete SPI-10 in Vi Negative Isolates
title Virulotyping of Salmonella Enterica Serovar Typhi Isolates from Pakistan: Absence of Complete SPI-10 in Vi Negative Isolates
spellingShingle Virulotyping of Salmonella Enterica Serovar Typhi Isolates from Pakistan: Absence of Complete SPI-10 in Vi Negative Isolates
Liaquat, Sadia
pathogenicity islands
variable-number
tandem repeats
gene sequence
multiplex pcr
antigen
fever
typhimurium
expression
title_short Virulotyping of Salmonella Enterica Serovar Typhi Isolates from Pakistan: Absence of Complete SPI-10 in Vi Negative Isolates
title_full Virulotyping of Salmonella Enterica Serovar Typhi Isolates from Pakistan: Absence of Complete SPI-10 in Vi Negative Isolates
title_fullStr Virulotyping of Salmonella Enterica Serovar Typhi Isolates from Pakistan: Absence of Complete SPI-10 in Vi Negative Isolates
title_full_unstemmed Virulotyping of Salmonella Enterica Serovar Typhi Isolates from Pakistan: Absence of Complete SPI-10 in Vi Negative Isolates
title_sort Virulotyping of Salmonella Enterica Serovar Typhi Isolates from Pakistan: Absence of Complete SPI-10 in Vi Negative Isolates
dc.creator.none.fl_str_mv Liaquat, Sadia
Sarwar, Yasra
Ali, Aamir
Haque, Abdul
Farooq, Muhammad
Martínez Ballesteros, Ilargi
Laorden Muñoz, Lorena
Garaizar Candina, Javier
Bikandi Bikandi, Joseba
author Liaquat, Sadia
author_facet Liaquat, Sadia
Sarwar, Yasra
Ali, Aamir
Haque, Abdul
Farooq, Muhammad
Martínez Ballesteros, Ilargi
Laorden Muñoz, Lorena
Garaizar Candina, Javier
Bikandi Bikandi, Joseba
author_role author
author2 Sarwar, Yasra
Ali, Aamir
Haque, Abdul
Farooq, Muhammad
Martínez Ballesteros, Ilargi
Laorden Muñoz, Lorena
Garaizar Candina, Javier
Bikandi Bikandi, Joseba
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv pathogenicity islands
variable-number
tandem repeats
gene sequence
multiplex pcr
antigen
fever
typhimurium
expression
topic pathogenicity islands
variable-number
tandem repeats
gene sequence
multiplex pcr
antigen
fever
typhimurium
expression
description The pathogenesis of Salmonella enterica serovar Typhi (S. Typhi), the cause of typhoid fever in humans, is mainly attributed to the acquisition of horizontally acquired DNA elements. Salmonella pathogenicity islands (SPIs) are indubitably the most important form of horizontally acquired DNA with respect to pathogenesis of this bacterium. The insertion or deletion of any of these transferrable SPIs may have impact on the virulence potential of S. Typhi. In this study, the virulence potential and genetic relatedness of 35 S. Typhi isolates, collected from 2004 to 2013 was determined by identification of SPI and non-SPI virulence factors through a combination of techniques including virulotyping, Whole Genome Sequencing (WGS), and Variable Number of Tandem Repeats (VNTR) profiling. In order to determine the virulence potential of local S. Typhi isolates, 56 virulence related genes were studied by PCR. These genes are located in the core as well as accessory genome (SPIs and plasmid). Major variations among studied virulence determinants were found in case of SPI-7 and SPI-10 associated genes. On the basis of presence of virulence related genes, the studied S. Typhi isolates from Pakistan were clustered into two virulotypes Vi-positive and Vi-negative. Interestingly, SPI-7 and SPI-10 were collectively absent or present in Vinegative and Vi-positive strains, respectively. Two Vi-negative and 11 Vi-positive S. Typhi strains were also analyzed by whole genome sequencing (WGS) and their results supported the PCR results. Genetic diversity was tested by VNTR-based molecular typing. All 35 isolates were clustered into five groups. Overall, all Vi-negative isolates were placed in a single group (T5) whereas Vi-positive isolates were grouped into four types. Vi-negative and Vi-positive isolates were mutually exclusive. This is the first report on the comparative distribution of SPI and non-SPI related virulence genes in Vi-negative and Vi-positive S. Typhi isolates with an important finding that SPI-10 is absent in all Vi-negative isolates.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/30885
url http://hdl.handle.net/10810/30885
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0006839
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869417306132578304
score 15,300719

Registos relacionados