Heterogeneity of Moraxella isolates found in the nasal cavities of piglets
Previous studies have shown that the genus Moraxella is commonly present in the nasal microbiota of swine. In this study, 51 isolates of Moraxella were obtained from nasal swabs from 3 to 4 week old piglets, which represented 26 different fingerprintings by enterobacterial repetitive intergenic cons...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:252423 |
| Acceso en línea: | https://ddd.uab.cat/record/252423 https://dx.doi.org/urn:doi:10.1186/s12917-020-2250-9 |
| Access Level: | acceso abierto |
| Palabra clave: | Swine Nasal microbiota Virulence Bacterial diversity Moraxella |
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Heterogeneity of Moraxella isolates found in the nasal cavities of pigletsLópez-Serrano, Sergi|||0000-0002-0968-7713Galofré Milà, NuriaCosta-Hurtado, MarPérez de Rozas, Ana|||0000-0002-5728-7642Aragón, Virginia|||0000-0002-3470-6015SwineNasal microbiotaVirulenceBacterial diversityMoraxellaPrevious studies have shown that the genus Moraxella is commonly present in the nasal microbiota of swine. In this study, 51 isolates of Moraxella were obtained from nasal swabs from 3 to 4 week old piglets, which represented 26 different fingerprintings by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Whole 16S rRNA gene sequencing allowed the identification at species level of the Moraxella spp. isolates. The majority of the field strains were identified as Moraxella pluranimalium, but Moraxella porci was also detected. In addition, a cluster of 7 strains did not group with any described Moraxella species, probably representing a new species. Subsequent phenotypic characterization indicated that strains of Moraxella pluranimalium were mainly sensitive to serum complement, while the cluster representing the putative new species was highly resistant. Biofilm formation capacity was very variable among the Moraxella spp. isolates, while adherence to epithelial cell lines was similar among selected strains. Additionally, variability was also observed in the association of selected strains to porcine alveolar macrophages. Antimicrobial tests evidenced the existence of multidrug-resistance in the strains. In summary, phenotypic characterization revealed heterogeneity among Moraxella strains from the nasal cavity of piglets. Strains with pathogenic potential were detected as well as those that may be commensal members of the nasal microbiota. However, the role of Moraxella in porcine diseases and health should be further evaluated. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/252423https://dx.doi.org/urn:doi:10.1186/s12917-020-2250-9reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 AGL2013-45662Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 AGL2016-77361open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2524232026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Heterogeneity of Moraxella isolates found in the nasal cavities of piglets |
| title |
Heterogeneity of Moraxella isolates found in the nasal cavities of piglets |
| spellingShingle |
Heterogeneity of Moraxella isolates found in the nasal cavities of piglets López-Serrano, Sergi|||0000-0002-0968-7713 Swine Nasal microbiota Virulence Bacterial diversity Moraxella |
| title_short |
Heterogeneity of Moraxella isolates found in the nasal cavities of piglets |
| title_full |
Heterogeneity of Moraxella isolates found in the nasal cavities of piglets |
| title_fullStr |
Heterogeneity of Moraxella isolates found in the nasal cavities of piglets |
| title_full_unstemmed |
Heterogeneity of Moraxella isolates found in the nasal cavities of piglets |
| title_sort |
Heterogeneity of Moraxella isolates found in the nasal cavities of piglets |
| dc.creator.none.fl_str_mv |
López-Serrano, Sergi|||0000-0002-0968-7713 Galofré Milà, Nuria Costa-Hurtado, Mar Pérez de Rozas, Ana|||0000-0002-5728-7642 Aragón, Virginia|||0000-0002-3470-6015 |
| author |
López-Serrano, Sergi|||0000-0002-0968-7713 |
| author_facet |
López-Serrano, Sergi|||0000-0002-0968-7713 Galofré Milà, Nuria Costa-Hurtado, Mar Pérez de Rozas, Ana|||0000-0002-5728-7642 Aragón, Virginia|||0000-0002-3470-6015 |
| author_role |
author |
| author2 |
Galofré Milà, Nuria Costa-Hurtado, Mar Pérez de Rozas, Ana|||0000-0002-5728-7642 Aragón, Virginia|||0000-0002-3470-6015 |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Swine Nasal microbiota Virulence Bacterial diversity Moraxella |
| topic |
Swine Nasal microbiota Virulence Bacterial diversity Moraxella |
| description |
Previous studies have shown that the genus Moraxella is commonly present in the nasal microbiota of swine. In this study, 51 isolates of Moraxella were obtained from nasal swabs from 3 to 4 week old piglets, which represented 26 different fingerprintings by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Whole 16S rRNA gene sequencing allowed the identification at species level of the Moraxella spp. isolates. The majority of the field strains were identified as Moraxella pluranimalium, but Moraxella porci was also detected. In addition, a cluster of 7 strains did not group with any described Moraxella species, probably representing a new species. Subsequent phenotypic characterization indicated that strains of Moraxella pluranimalium were mainly sensitive to serum complement, while the cluster representing the putative new species was highly resistant. Biofilm formation capacity was very variable among the Moraxella spp. isolates, while adherence to epithelial cell lines was similar among selected strains. Additionally, variability was also observed in the association of selected strains to porcine alveolar macrophages. Antimicrobial tests evidenced the existence of multidrug-resistance in the strains. In summary, phenotypic characterization revealed heterogeneity among Moraxella strains from the nasal cavity of piglets. Strains with pathogenic potential were detected as well as those that may be commensal members of the nasal microbiota. However, the role of Moraxella in porcine diseases and health should be further evaluated. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2 2020-01-01 2020 2020-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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https://ddd.uab.cat/record/252423 https://dx.doi.org/urn:doi:10.1186/s12917-020-2250-9 |
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https://ddd.uab.cat/record/252423 https://dx.doi.org/urn:doi:10.1186/s12917-020-2250-9 |
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Inglés eng |
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Inglés |
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eng |
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Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 AGL2013-45662 Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 AGL2016-77361 |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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Universitat Autònoma de Barcelona |
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