Identification and characterization of amphipathic antimicrobial peptides with broad spectrum activity against multi-drug resistant bacteria

Antimicrobial peptides (AMPs) are potential alternatives to antibiotics given the reduced likelihood of resistance and their high selectivity towards bacteria. AMPs with activity against antibiotic-resistant bacteria have been reported. The aim of this study is to characterize the activity of novel...

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Autores: López Siles, Mireia, Tajuelo, Ana, Caravaca Fuentes, Pau, Planas i Grabuleda, Marta, Feliu Soley, Lidia, McConnell, Michael J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/28128
Acceso en línea:http://hdl.handle.net/10256/28128
Access Level:acceso abierto
Palabra clave:Antibiòtics pèptids
Peptide antibiotics
Microorganismes -- Resistència als medicaments
Drug resistance in microorganisms
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spelling Identification and characterization of amphipathic antimicrobial peptides with broad spectrum activity against multi-drug resistant bacteriaLópez Siles, MireiaTajuelo, AnaCaravaca Fuentes, PauPlanas i Grabuleda, MartaFeliu Soley, LidiaMcConnell, Michael J.Antibiòtics pèptidsPeptide antibioticsMicroorganismes -- Resistència als medicamentsDrug resistance in microorganismsAntimicrobial peptides (AMPs) are potential alternatives to antibiotics given the reduced likelihood of resistance and their high selectivity towards bacteria. AMPs with activity against antibiotic-resistant bacteria have been reported. The aim of this study is to characterize the activity of novel BP100 analogues against multidrug-resistant bacteria. Eleven bacterial strains representing five pathogenic species were used to evaluate the antimicrobial activity of 26 peptides. An initial screen was performed at 50 µg/ml, and those peptides that inhibited ≈90 % of growth of all strains were selected. Minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC), inhibition in biofilm formation, time kill assays, stability in human serum and in vivo toxicity were assessed. BP607, BP76 and BP145, had broad activity against multidrug-resistant bacteria. MICs ranged between 3.13 and 50 µg/ml, whereas MBCs ranged between 6.25 and 100 µg/ml. Acinetobacter baumannii, Klebsiella pneumoniae and Escherichia coli were the most susceptible species. At 2x the MIC, all compounds were bactericidal after 6h. BP76 inhibited ≥ 76.77 % of K. pneumoniae and E. coli biofilm formation at subinhibitory concentrations. BP145 had improved serum stability and lower toxicity compared to BP607. In conclusion, BP145 and BP76 demonstrate broad antimicrobial activity, are active at non-toxic concentrations, feature bactericidal activity at 6h and inhibit biofilm formationMLS is a Serra Húnter Fellow, and acknowledges support from the Catalan Research Agency (AGAUR, 2023 PROD 00034) and “La Caixa” foundation (Grant LCF/TR/CI23/56000019). AT is supported by a FPU grant (FPU20/03261). PCF is supported by a FPU grant (FPU20/07152). MJM is supported by grants from the Instituto de Salud Carlos III (MPY 516/19 and MPY 380/18)Elsevier2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/28128http://hdl.handle.net/10256/28128Current Research in Microbial Sciences, 2025, vol. 8, art. núm. 100363Articles publicats (D-Q)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.crmicr.2025.100363info:eu-repo/semantics/altIdentifier/eissn/2666-5174Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/281282026-05-29T05:05:01Z
dc.title.none.fl_str_mv Identification and characterization of amphipathic antimicrobial peptides with broad spectrum activity against multi-drug resistant bacteria
title Identification and characterization of amphipathic antimicrobial peptides with broad spectrum activity against multi-drug resistant bacteria
spellingShingle Identification and characterization of amphipathic antimicrobial peptides with broad spectrum activity against multi-drug resistant bacteria
López Siles, Mireia
Antibiòtics pèptids
Peptide antibiotics
Microorganismes -- Resistència als medicaments
Drug resistance in microorganisms
title_short Identification and characterization of amphipathic antimicrobial peptides with broad spectrum activity against multi-drug resistant bacteria
title_full Identification and characterization of amphipathic antimicrobial peptides with broad spectrum activity against multi-drug resistant bacteria
title_fullStr Identification and characterization of amphipathic antimicrobial peptides with broad spectrum activity against multi-drug resistant bacteria
title_full_unstemmed Identification and characterization of amphipathic antimicrobial peptides with broad spectrum activity against multi-drug resistant bacteria
title_sort Identification and characterization of amphipathic antimicrobial peptides with broad spectrum activity against multi-drug resistant bacteria
dc.creator.none.fl_str_mv López Siles, Mireia
Tajuelo, Ana
Caravaca Fuentes, Pau
Planas i Grabuleda, Marta
Feliu Soley, Lidia
McConnell, Michael J.
author López Siles, Mireia
author_facet López Siles, Mireia
Tajuelo, Ana
Caravaca Fuentes, Pau
Planas i Grabuleda, Marta
Feliu Soley, Lidia
McConnell, Michael J.
author_role author
author2 Tajuelo, Ana
Caravaca Fuentes, Pau
Planas i Grabuleda, Marta
Feliu Soley, Lidia
McConnell, Michael J.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Antibiòtics pèptids
Peptide antibiotics
Microorganismes -- Resistència als medicaments
Drug resistance in microorganisms
topic Antibiòtics pèptids
Peptide antibiotics
Microorganismes -- Resistència als medicaments
Drug resistance in microorganisms
description Antimicrobial peptides (AMPs) are potential alternatives to antibiotics given the reduced likelihood of resistance and their high selectivity towards bacteria. AMPs with activity against antibiotic-resistant bacteria have been reported. The aim of this study is to characterize the activity of novel BP100 analogues against multidrug-resistant bacteria. Eleven bacterial strains representing five pathogenic species were used to evaluate the antimicrobial activity of 26 peptides. An initial screen was performed at 50 µg/ml, and those peptides that inhibited ≈90 % of growth of all strains were selected. Minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC), inhibition in biofilm formation, time kill assays, stability in human serum and in vivo toxicity were assessed. BP607, BP76 and BP145, had broad activity against multidrug-resistant bacteria. MICs ranged between 3.13 and 50 µg/ml, whereas MBCs ranged between 6.25 and 100 µg/ml. Acinetobacter baumannii, Klebsiella pneumoniae and Escherichia coli were the most susceptible species. At 2x the MIC, all compounds were bactericidal after 6h. BP76 inhibited ≥ 76.77 % of K. pneumoniae and E. coli biofilm formation at subinhibitory concentrations. BP145 had improved serum stability and lower toxicity compared to BP607. In conclusion, BP145 and BP76 demonstrate broad antimicrobial activity, are active at non-toxic concentrations, feature bactericidal activity at 6h and inhibit biofilm formation
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/28128
http://hdl.handle.net/10256/28128
url http://hdl.handle.net/10256/28128
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.crmicr.2025.100363
info:eu-repo/semantics/altIdentifier/eissn/2666-5174
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Current Research in Microbial Sciences, 2025, vol. 8, art. núm. 100363
Articles publicats (D-Q)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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