Functional stability and community dynamics during spring and autumn seasons over 3 years in Camargue microbial mats
Microbial mats are complex biofilms in which the major element cycles are represented at a millimeter scale. In this study, community variability within microbial mats from the Camargue wetlands (Rhone Delta, southern France) were analyzed over 3 years during two different seasons (spring and autumn...
| Autores: | , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/121320 |
| Acceso en línea: | https://hdl.handle.net/2445/121320 |
| Access Level: | acceso abierto |
| Palabra clave: | Genètica microbiana Genòmica Microbiologia Microbial genetics Genomics Microbiology |
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Functional stability and community dynamics during spring and autumn seasons over 3 years in Camargue microbial matsBerlanga Herranz, MercedesPalau de Miguel, MontserratGuerrero, Ricardo, 1943-Genètica microbianaGenòmicaMicrobiologiaMicrobial geneticsGenomicsMicrobiologyMicrobial mats are complex biofilms in which the major element cycles are represented at a millimeter scale. In this study, community variability within microbial mats from the Camargue wetlands (Rhone Delta, southern France) were analyzed over 3 years during two different seasons (spring and autumn) and at different layers of the mat (0-2, 2-4, and 4-6 mm). To assess bacterial diversity in the mats, amplicons of the V1-V2 region of the 16S rRNA gene were sequenced. The community's functionality was characterized using two approaches: (i) inferred functionality through 16S rRNA amplicons genes according to PICRUSt, and (ii) a shotgun metagenomic analysis. Based on the reads distinguished, microbial communities were dominated by Bacteria ( 94%), followed by Archaea ( 4%) and Eukarya ( 1%). The major phyla of Bacteria were Proteobacteria, Bacteroidetes, Spirochaetes, Actinobacteria, Firmicutes, and Cyanobacteria, which together represented 70-80% of the total population detected. The phylum Euryarchaeota represented 80% of the Archaea identified. These results showed that the total bacterial diversity from the Camargue microbial mats was not significantly affected by seasonal changes at the studied location; however, there were differences among layers, especially between the 0-2 mm layer and the other two layers. PICRUSt and shotgun metagenomic analyses revealed similar general biological processes in all samples analyzed, by season and depth, indicating that different layers were functionally stable, although some taxa changed during the spring and autumn seasons over the 3 years. Several gene families and pathways were tracked with the oxic-anoxic gradient of the layers. Genes directly involved in photosynthesis (KO, KEGG Orthology) were significantly more abundant in the top layer (0-2 mm) than in the lower layers (2-4 and 4-6 mm). In the anoxic layers, the presence of ferredoxins likely reflected the variation of redox reactions required for anaerobic respiration. Sulfatase genes had the highest relative abundance below 2 mm. Finally, chemotaxis signature genes peaked sharply at the oxic/photic and transitional oxic-anoxic boundary. This functional differentiation reflected the taxonomic diversity of the different layers of the mat.Frontiers Media2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/121320Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fmicb.2017.02619Frontiers in Microbiology, 2017, vol. 8, p. 2619https://doi.org/10.3389/fmicb.2017.02619cc-by (c) Berlanga Herranz, Mercedes et al., 2017http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1213202026-05-27T06:46:51Z |
| dc.title.none.fl_str_mv |
Functional stability and community dynamics during spring and autumn seasons over 3 years in Camargue microbial mats |
| title |
Functional stability and community dynamics during spring and autumn seasons over 3 years in Camargue microbial mats |
| spellingShingle |
Functional stability and community dynamics during spring and autumn seasons over 3 years in Camargue microbial mats Berlanga Herranz, Mercedes Genètica microbiana Genòmica Microbiologia Microbial genetics Genomics Microbiology |
| title_short |
Functional stability and community dynamics during spring and autumn seasons over 3 years in Camargue microbial mats |
| title_full |
Functional stability and community dynamics during spring and autumn seasons over 3 years in Camargue microbial mats |
| title_fullStr |
Functional stability and community dynamics during spring and autumn seasons over 3 years in Camargue microbial mats |
| title_full_unstemmed |
Functional stability and community dynamics during spring and autumn seasons over 3 years in Camargue microbial mats |
| title_sort |
Functional stability and community dynamics during spring and autumn seasons over 3 years in Camargue microbial mats |
| dc.creator.none.fl_str_mv |
Berlanga Herranz, Mercedes Palau de Miguel, Montserrat Guerrero, Ricardo, 1943- |
| author |
Berlanga Herranz, Mercedes |
| author_facet |
Berlanga Herranz, Mercedes Palau de Miguel, Montserrat Guerrero, Ricardo, 1943- |
| author_role |
author |
| author2 |
Palau de Miguel, Montserrat Guerrero, Ricardo, 1943- |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
Genètica microbiana Genòmica Microbiologia Microbial genetics Genomics Microbiology |
| topic |
Genètica microbiana Genòmica Microbiologia Microbial genetics Genomics Microbiology |
| description |
Microbial mats are complex biofilms in which the major element cycles are represented at a millimeter scale. In this study, community variability within microbial mats from the Camargue wetlands (Rhone Delta, southern France) were analyzed over 3 years during two different seasons (spring and autumn) and at different layers of the mat (0-2, 2-4, and 4-6 mm). To assess bacterial diversity in the mats, amplicons of the V1-V2 region of the 16S rRNA gene were sequenced. The community's functionality was characterized using two approaches: (i) inferred functionality through 16S rRNA amplicons genes according to PICRUSt, and (ii) a shotgun metagenomic analysis. Based on the reads distinguished, microbial communities were dominated by Bacteria ( 94%), followed by Archaea ( 4%) and Eukarya ( 1%). The major phyla of Bacteria were Proteobacteria, Bacteroidetes, Spirochaetes, Actinobacteria, Firmicutes, and Cyanobacteria, which together represented 70-80% of the total population detected. The phylum Euryarchaeota represented 80% of the Archaea identified. These results showed that the total bacterial diversity from the Camargue microbial mats was not significantly affected by seasonal changes at the studied location; however, there were differences among layers, especially between the 0-2 mm layer and the other two layers. PICRUSt and shotgun metagenomic analyses revealed similar general biological processes in all samples analyzed, by season and depth, indicating that different layers were functionally stable, although some taxa changed during the spring and autumn seasons over the 3 years. Several gene families and pathways were tracked with the oxic-anoxic gradient of the layers. Genes directly involved in photosynthesis (KO, KEGG Orthology) were significantly more abundant in the top layer (0-2 mm) than in the lower layers (2-4 and 4-6 mm). In the anoxic layers, the presence of ferredoxins likely reflected the variation of redox reactions required for anaerobic respiration. Sulfatase genes had the highest relative abundance below 2 mm. Finally, chemotaxis signature genes peaked sharply at the oxic/photic and transitional oxic-anoxic boundary. This functional differentiation reflected the taxonomic diversity of the different layers of the mat. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/121320 |
| url |
https://hdl.handle.net/2445/121320 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
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Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2017.02619 Frontiers in Microbiology, 2017, vol. 8, p. 2619 https://doi.org/10.3389/fmicb.2017.02619 |
| dc.rights.none.fl_str_mv |
cc-by (c) Berlanga Herranz, Mercedes et al., 2017 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess |
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cc-by (c) Berlanga Herranz, Mercedes et al., 2017 http://creativecommons.org/licenses/by/3.0/es |
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openAccess |
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application/pdf |
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Frontiers Media |
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Frontiers Media |
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Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona |
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Universidad de Barcelona |
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Dipòsit Digital de la UB |
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Dipòsit Digital de la UB |
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