Nurr1 protein Is required for N-methyl-d-aspartic acid (NMDA) receptor-mediated neuronal survival

This work was supported by grants from the Ministerio de Ciencia e Innovación (Grants SAF2008-01904, SAF2011-30281, and RENEVAS) and CIBERNED (Grant CB06/05/0042) (to J. R. A) and the Ministerio de Ciencia e Innovación (Grant SAF2010-20925) (to C. A. S.).

Detalles Bibliográficos
Autores: Barneda-Zahonero, Bruna, Servitja, Joan-Marc, Badiola, Nahuai, Miñano Molina, Alfredo Jesús, Fadó Andrés, Rut, Saura, Carlos A., Rodríguez-Álvarez, José
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12328/2510
Acceso en línea:http://hdl.handle.net/20.500.12328/2510
https://dx.doi.org/10.1074/jbc.M111.272427
Access Level:acceso abierto
Palabra clave:Neurones
Cervell -- Evolució
Genomes
Apoptosi
Neuronas
Cerebro -- Evolución
Genomas
Apoptosis
Neurons
Brain -- Evolution
61
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spelling Nurr1 protein Is required for N-methyl-d-aspartic acid (NMDA) receptor-mediated neuronal survivalBarneda-Zahonero, BrunaServitja, Joan-MarcBadiola, NahuaiMiñano Molina, Alfredo JesúsFadó Andrés, RutSaura, Carlos A.Rodríguez-Álvarez, JoséNeuronesCervell -- EvolucióGenomesApoptosiNeuronasCerebro -- EvoluciónGenomasApoptosisNeuronsBrain -- EvolutionGenomesApoptosis61This work was supported by grants from the Ministerio de Ciencia e Innovación (Grants SAF2008-01904, SAF2011-30281, and RENEVAS) and CIBERNED (Grant CB06/05/0042) (to J. R. A) and the Ministerio de Ciencia e Innovación (Grant SAF2010-20925) (to C. A. S.).NMDA receptor (NMDAR) stimulation promotes neuronal survival during brain development. Cerebellar granule cells (CGCs) need NMDAR stimulation to survive and develop. These neurons differentiate and mature during its migration from the external granular layer to the internal granular layer, and lack of excitatory inputs triggers their apoptotic death. It is possible to mimic this process in vitro by culturing CGCs in low KCl concentrations (5 mm) in the presence or absence of NMDA. Using this experimental approach, we have obtained whole genome expression profiles after 3 and 8 h of NMDA addition to identify genes involved in NMDA-mediated survival of CGCs. One of the identified genes was Nurr1, a member of the orphan nuclear receptor subfamily Nr4a. Our results report a direct regulation of Nurr1 by CREB after NMDAR stimulation. ChIP assay confirmed CREB binding to Nurr1 promoter, whereas CREB shRNA blocked NMDA-mediated increase in Nurr1 expression. Moreover, we show that Nurr1 is important for NMDAR survival effect. We show that Nurr1 binds to Bdnf promoter IV and that silencing Nurr1 by shRNA leads to a decrease in brain-derived neurotrophic factor (BDNF) protein levels and a reduction of NMDA neuroprotective effect. Also, we report that Nurr1 and BDNF show a similar expression pattern during postnatal cerebellar development. Thus, we conclude that Nurr1 is a downstream target of CREB and that it is responsible for the NMDA-mediated increase in BDNF, which is necessary for the NMDA-mediated prosurvival effect on neurons.info:eu-repo/semantics/publishedVersionElsevier2012info:eu-repo/semantics/article12http://hdl.handle.net/20.500.12328/2510https://dx.doi.org/10.1074/jbc.M111.272427reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésJournal of Biological Chemistry287;14info:eu-repo/grantAgreement/ES/MICINN/SAF2008-01904info:eu-repo/grantAgreement/ES/MICINN/SAF2011-30281info:eu-repo/grantAgreement/ES/MICINN/SAF2010-20925This is an open access article under the CC BY license.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12328/25102026-05-29T05:05:01Z
dc.title.none.fl_str_mv Nurr1 protein Is required for N-methyl-d-aspartic acid (NMDA) receptor-mediated neuronal survival
title Nurr1 protein Is required for N-methyl-d-aspartic acid (NMDA) receptor-mediated neuronal survival
spellingShingle Nurr1 protein Is required for N-methyl-d-aspartic acid (NMDA) receptor-mediated neuronal survival
Barneda-Zahonero, Bruna
Neurones
Cervell -- Evolució
Genomes
Apoptosi
Neuronas
Cerebro -- Evolución
Genomas
Apoptosis
Neurons
Brain -- Evolution
Genomes
Apoptosis
61
title_short Nurr1 protein Is required for N-methyl-d-aspartic acid (NMDA) receptor-mediated neuronal survival
title_full Nurr1 protein Is required for N-methyl-d-aspartic acid (NMDA) receptor-mediated neuronal survival
title_fullStr Nurr1 protein Is required for N-methyl-d-aspartic acid (NMDA) receptor-mediated neuronal survival
title_full_unstemmed Nurr1 protein Is required for N-methyl-d-aspartic acid (NMDA) receptor-mediated neuronal survival
title_sort Nurr1 protein Is required for N-methyl-d-aspartic acid (NMDA) receptor-mediated neuronal survival
dc.creator.none.fl_str_mv Barneda-Zahonero, Bruna
Servitja, Joan-Marc
Badiola, Nahuai
Miñano Molina, Alfredo Jesús
Fadó Andrés, Rut
Saura, Carlos A.
Rodríguez-Álvarez, José
author Barneda-Zahonero, Bruna
author_facet Barneda-Zahonero, Bruna
Servitja, Joan-Marc
Badiola, Nahuai
Miñano Molina, Alfredo Jesús
Fadó Andrés, Rut
Saura, Carlos A.
Rodríguez-Álvarez, José
author_role author
author2 Servitja, Joan-Marc
Badiola, Nahuai
Miñano Molina, Alfredo Jesús
Fadó Andrés, Rut
Saura, Carlos A.
Rodríguez-Álvarez, José
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Neurones
Cervell -- Evolució
Genomes
Apoptosi
Neuronas
Cerebro -- Evolución
Genomas
Apoptosis
Neurons
Brain -- Evolution
Genomes
Apoptosis
61
topic Neurones
Cervell -- Evolució
Genomes
Apoptosi
Neuronas
Cerebro -- Evolución
Genomas
Apoptosis
Neurons
Brain -- Evolution
Genomes
Apoptosis
61
description This work was supported by grants from the Ministerio de Ciencia e Innovación (Grants SAF2008-01904, SAF2011-30281, and RENEVAS) and CIBERNED (Grant CB06/05/0042) (to J. R. A) and the Ministerio de Ciencia e Innovación (Grant SAF2010-20925) (to C. A. S.).
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12328/2510
https://dx.doi.org/10.1074/jbc.M111.272427
url http://hdl.handle.net/20.500.12328/2510
https://dx.doi.org/10.1074/jbc.M111.272427
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Biological Chemistry
287;14
info:eu-repo/grantAgreement/ES/MICINN/SAF2008-01904
info:eu-repo/grantAgreement/ES/MICINN/SAF2011-30281
info:eu-repo/grantAgreement/ES/MICINN/SAF2010-20925
dc.rights.none.fl_str_mv This is an open access article under the CC BY license.
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv This is an open access article under the CC BY license.
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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