Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank

One of the main concerns in human milk banks (HMB) is the transmission of human cytomegalovirus (HCMV) that could be present in the milk of infected women. There are consistent data showing that this virus is destroyed by Holder pasteurization (62.5 degrees C for 30 min), but there is a lack of info...

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Autores: Gayà, Antoni, Ritta, Massimo, Lembo, David, Tonetto, Paola, Cresi, Francesco, Sottemano, Stefano, Bertino, Enrico, Moro, Guido E., Calvo Benito, Javier, Donalisio, Manuela
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositorio:Docusalut
Idioma:inglés
OAI Identifier:oai:docusalut.com:20.500.13003/19442
Acceso en línea:https://hdl.handle.net/20.500.13003/19442
Access Level:acceso abierto
Palabra clave:holder pasteurization
donor human milk
viral inactivation
human milk bank
breast milk
cytomegalovirus
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spelling Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk BankGayà, AntoniRitta, MassimoLembo, DavidTonetto, PaolaCresi, FrancescoSottemano, StefanoBertino, EnricoMoro, Guido E.Calvo Benito, JavierDonalisio, Manuelaholder pasteurizationdonor human milkviral inactivationhuman milk bankbreast milkcytomegalovirusOne of the main concerns in human milk banks (HMB) is the transmission of human cytomegalovirus (HCMV) that could be present in the milk of infected women. There are consistent data showing that this virus is destroyed by Holder pasteurization (62.5 degrees C for 30 min), but there is a lack of information about the response of the virus to the treatment at lower temperatures in strict HMB conditions. In order to analyze the effectiveness of different temperatures of pasteurization to eliminate HCMV in human milk, a preliminary assay was performed incubating HCMV-spiked raw milk samples from donor mothers at tested temperatures in a PCR thermocycler and the viral infectivity was assayed on cell cultures. No signs of viral replication were observed after treatments at temperatures equal or >53 degrees C for 30, 20, and 10 min, 58 degrees C for 5 min, 59 degrees C for 2 min, and 60 degrees C for 1 min. These data were confirmed in a pasteurizer-like model introducing HCMV-spiked milk in disposable baby bottles. No viral infectivity was detected on cell cultures after heating treatment of milk for 30 min at temperatures from 56 to 60 degrees C. Thus, our results show that by using conventional pasteurization conditions, temperatures in the range of 56-60 degrees C are enough to inactivate HCMV. Consequently, we consider that, in order to provide a higher quality product, the current recommendation to pasteurize both mother's own milk and donated milk at 62.5 degrees C must be re-evaluated.Frontiers Media Sa20212021-07-2720212021-07-27research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.13003/19442reponame:Docusalutinstname:Conselleria de Salut i Consum del Govern de les Illes BalearsInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docusalut.com:20.500.13003/194422026-06-22T12:44:07Z
dc.title.none.fl_str_mv Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
title Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
spellingShingle Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
Gayà, Antoni
holder pasteurization
donor human milk
viral inactivation
human milk bank
breast milk
cytomegalovirus
title_short Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
title_full Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
title_fullStr Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
title_full_unstemmed Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
title_sort Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
dc.creator.none.fl_str_mv Gayà, Antoni
Ritta, Massimo
Lembo, David
Tonetto, Paola
Cresi, Francesco
Sottemano, Stefano
Bertino, Enrico
Moro, Guido E.
Calvo Benito, Javier
Donalisio, Manuela
author Gayà, Antoni
author_facet Gayà, Antoni
Ritta, Massimo
Lembo, David
Tonetto, Paola
Cresi, Francesco
Sottemano, Stefano
Bertino, Enrico
Moro, Guido E.
Calvo Benito, Javier
Donalisio, Manuela
author_role author
author2 Ritta, Massimo
Lembo, David
Tonetto, Paola
Cresi, Francesco
Sottemano, Stefano
Bertino, Enrico
Moro, Guido E.
Calvo Benito, Javier
Donalisio, Manuela
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv holder pasteurization
donor human milk
viral inactivation
human milk bank
breast milk
cytomegalovirus
topic holder pasteurization
donor human milk
viral inactivation
human milk bank
breast milk
cytomegalovirus
description One of the main concerns in human milk banks (HMB) is the transmission of human cytomegalovirus (HCMV) that could be present in the milk of infected women. There are consistent data showing that this virus is destroyed by Holder pasteurization (62.5 degrees C for 30 min), but there is a lack of information about the response of the virus to the treatment at lower temperatures in strict HMB conditions. In order to analyze the effectiveness of different temperatures of pasteurization to eliminate HCMV in human milk, a preliminary assay was performed incubating HCMV-spiked raw milk samples from donor mothers at tested temperatures in a PCR thermocycler and the viral infectivity was assayed on cell cultures. No signs of viral replication were observed after treatments at temperatures equal or >53 degrees C for 30, 20, and 10 min, 58 degrees C for 5 min, 59 degrees C for 2 min, and 60 degrees C for 1 min. These data were confirmed in a pasteurizer-like model introducing HCMV-spiked milk in disposable baby bottles. No viral infectivity was detected on cell cultures after heating treatment of milk for 30 min at temperatures from 56 to 60 degrees C. Thus, our results show that by using conventional pasteurization conditions, temperatures in the range of 56-60 degrees C are enough to inactivate HCMV. Consequently, we consider that, in order to provide a higher quality product, the current recommendation to pasteurize both mother's own milk and donated milk at 62.5 degrees C must be re-evaluated.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-07-27
2021
2021-07-27
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.13003/19442
url https://hdl.handle.net/20.500.13003/19442
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media Sa
publisher.none.fl_str_mv Frontiers Media Sa
dc.source.none.fl_str_mv reponame:Docusalut
instname:Conselleria de Salut i Consum del Govern de les Illes Balears
instname_str Conselleria de Salut i Consum del Govern de les Illes Balears
reponame_str Docusalut
collection Docusalut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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