Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels

[EN]Over the last decades, novel therapeutic tools for osteochondral regeneration have arisen from the combination of mesenchymal stromal cells (MSCs) and highly specialized smart biomaterials, such as hydrogel-forming elastin-like recombinamers (ELRs), which could serve as cell-carriers. Herein, we...

ver descrição completa

Detalhes bibliográficos
Autores: Pescador Hernández, David, Ibáñez-Fonseca, Arturo, Sánchez-Guijo Martín, Fermín, García Briñón, Jesús María, Arias, Francisco Javier, Muntión Olave, María Sandra, Hernández Fuentevilla, Cristina Natalia, Girotti, Alessandra, Alonso, Matilde, Cañizo Fernández-Roldán, María Consuelo del, Rodríguez-Cabello, José Carlos, Blanco Blanco, Juan Francisco
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2017
País:España
Recursos:Universidad de Salamanca (USAL)
Repositório:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/146426
Acesso em linha:http://hdl.handle.net/10366/146426
Access Level:Acceso aberto
Palavra-chave:Hyaline cartilage
Xenogeneic
Mesenchymal stromal cells
Elastin
Bioactive hydrogels
Mitochondrial antibody
Mitochondrial Membranes
Mesenchymal Stromal Cells
Hyaline Cartilage
2407 Biología Celular
3213.15 Traumatología
membranas mitocondriales
cartílago hialino
células del estroma mesenquimatoso
id ES_b4bcea07f2477d63cbc736c5f56b42bc
oai_identifier_str oai:gredos.usal.es:10366/146426
network_acronym_str ES
network_name_str España
repository_id_str
spelling Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogelsPescador Hernández, DavidIbáñez-Fonseca, ArturoSánchez-Guijo Martín, FermínGarcía Briñón, Jesús MaríaArias, Francisco JavierMuntión Olave, María SandraHernández Fuentevilla, Cristina NataliaGirotti, AlessandraAlonso, MatildeCañizo Fernández-Roldán, María Consuelo delRodríguez-Cabello, José CarlosBlanco Blanco, Juan FranciscoHyaline cartilageXenogeneicMesenchymal stromal cellsElastinBioactive hydrogelsMitochondrial antibodyMitochondrial MembranesMesenchymal Stromal CellsHyaline Cartilage2407 Biología Celular3213.15 Traumatologíamembranas mitocondrialescartílago hialinocélulas del estroma mesenquimatoso[EN]Over the last decades, novel therapeutic tools for osteochondral regeneration have arisen from the combination of mesenchymal stromal cells (MSCs) and highly specialized smart biomaterials, such as hydrogel-forming elastin-like recombinamers (ELRs), which could serve as cell-carriers. Herein, we evaluate the delivery of xenogeneic human MSCs (hMSCs) within an injectable ELR-based hydrogel carrier for osteochondral regeneration in rabbits. First, a critical-size osteochondral defect was created in the femora of the animals and subsequently filled with the ELRbased hydrogel alone or with embedded hMSCs. Regeneration outcomes were evaluated after three months by gross assessment, magnetic resonance imaging and computed tomography, showing complete filling of the defect and the de novo formation of hyaline-like cartilage and subchondral bone in the hMSC-treated knees. Furthermore, histological sectioning and staining of every sample confirmed regeneration of the full cartilage thickness and early subchondral bone repair, which was more similar to the native cartilage in the case of the cell-loaded ELR-based hydrogel. Overall histological differences between the two groups were assessed semi-quantitatively using the Wakitani scale and found to be statistically significant (p < 0.05).Springer Link202120212017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/146426reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1464262026-06-07T06:28:51Z
dc.title.none.fl_str_mv Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels
title Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels
spellingShingle Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels
Pescador Hernández, David
Hyaline cartilage
Xenogeneic
Mesenchymal stromal cells
Elastin
Bioactive hydrogels
Mitochondrial antibody
Mitochondrial Membranes
Mesenchymal Stromal Cells
Hyaline Cartilage
2407 Biología Celular
3213.15 Traumatología
membranas mitocondriales
cartílago hialino
células del estroma mesenquimatoso
title_short Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels
title_full Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels
title_fullStr Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels
title_full_unstemmed Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels
title_sort Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels
dc.creator.none.fl_str_mv Pescador Hernández, David
Ibáñez-Fonseca, Arturo
Sánchez-Guijo Martín, Fermín
García Briñón, Jesús María
Arias, Francisco Javier
Muntión Olave, María Sandra
Hernández Fuentevilla, Cristina Natalia
Girotti, Alessandra
Alonso, Matilde
Cañizo Fernández-Roldán, María Consuelo del
Rodríguez-Cabello, José Carlos
Blanco Blanco, Juan Francisco
author Pescador Hernández, David
author_facet Pescador Hernández, David
Ibáñez-Fonseca, Arturo
Sánchez-Guijo Martín, Fermín
García Briñón, Jesús María
Arias, Francisco Javier
Muntión Olave, María Sandra
Hernández Fuentevilla, Cristina Natalia
Girotti, Alessandra
Alonso, Matilde
Cañizo Fernández-Roldán, María Consuelo del
Rodríguez-Cabello, José Carlos
Blanco Blanco, Juan Francisco
author_role author
author2 Ibáñez-Fonseca, Arturo
Sánchez-Guijo Martín, Fermín
García Briñón, Jesús María
Arias, Francisco Javier
Muntión Olave, María Sandra
Hernández Fuentevilla, Cristina Natalia
Girotti, Alessandra
Alonso, Matilde
Cañizo Fernández-Roldán, María Consuelo del
Rodríguez-Cabello, José Carlos
Blanco Blanco, Juan Francisco
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hyaline cartilage
Xenogeneic
Mesenchymal stromal cells
Elastin
Bioactive hydrogels
Mitochondrial antibody
Mitochondrial Membranes
Mesenchymal Stromal Cells
Hyaline Cartilage
2407 Biología Celular
3213.15 Traumatología
membranas mitocondriales
cartílago hialino
células del estroma mesenquimatoso
topic Hyaline cartilage
Xenogeneic
Mesenchymal stromal cells
Elastin
Bioactive hydrogels
Mitochondrial antibody
Mitochondrial Membranes
Mesenchymal Stromal Cells
Hyaline Cartilage
2407 Biología Celular
3213.15 Traumatología
membranas mitocondriales
cartílago hialino
células del estroma mesenquimatoso
description [EN]Over the last decades, novel therapeutic tools for osteochondral regeneration have arisen from the combination of mesenchymal stromal cells (MSCs) and highly specialized smart biomaterials, such as hydrogel-forming elastin-like recombinamers (ELRs), which could serve as cell-carriers. Herein, we evaluate the delivery of xenogeneic human MSCs (hMSCs) within an injectable ELR-based hydrogel carrier for osteochondral regeneration in rabbits. First, a critical-size osteochondral defect was created in the femora of the animals and subsequently filled with the ELRbased hydrogel alone or with embedded hMSCs. Regeneration outcomes were evaluated after three months by gross assessment, magnetic resonance imaging and computed tomography, showing complete filling of the defect and the de novo formation of hyaline-like cartilage and subchondral bone in the hMSC-treated knees. Furthermore, histological sectioning and staining of every sample confirmed regeneration of the full cartilage thickness and early subchondral bone repair, which was more similar to the native cartilage in the case of the cell-loaded ELR-based hydrogel. Overall histological differences between the two groups were assessed semi-quantitatively using the Wakitani scale and found to be statistically significant (p < 0.05).
publishDate 2017
dc.date.none.fl_str_mv 2017
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/146426
url http://hdl.handle.net/10366/146426
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Link
publisher.none.fl_str_mv Springer Link
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869417285979996160
score 15,301603

Registros relacionados