Insights into the Regulatory Roles of miRNAs in the Salivary Glands of the Soft Ticks Ornithodoros moubata and Ornithodoros erraticus

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by inhibiting or degrading messenger RNAs (mRNAs). In ticks, salivary miRNAs are proposed to play key roles in modulating host–vector interactions during blood feeding. Previously, we identified salivary miRNAs in Ornithodoro...

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Detalhes bibliográficos
Autores: Cano Argüelles, Ana Laura, Pérez Sánchez, Ricardo, Gallardo Escárate, Cristian, Vizcaíno-Marín, Rocío, González-Sánchez, María, Oleaga, Ana
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/394159
Acesso em linha:http://hdl.handle.net/10261/394159
Access Level:acceso abierto
Palavra-chave:Ornithodoros moubata
Ornithodoros erraticus
MicroRNA
Antagomir
Salivary glands
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spelling Insights into the Regulatory Roles of miRNAs in the Salivary Glands of the Soft Ticks Ornithodoros moubata and Ornithodoros erraticusCano Argüelles, Ana LauraPérez Sánchez, RicardoGallardo Escárate, CristianVizcaíno-Marín, RocíoGonzález-Sánchez, MaríaOleaga, AnaOrnithodoros moubataOrnithodoros erraticusMicroRNAAntagomirSalivary glandsMicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by inhibiting or degrading messenger RNAs (mRNAs). In ticks, salivary miRNAs are proposed to play key roles in modulating host–vector interactions during blood feeding. Previously, we identified salivary miRNAs in Ornithodoros moubata and Ornithodoros erraticus, major vectors of African swine fever and tick-borne human relapsing fever. In this study, we investigated the regulatory roles of salivary miRNAs in tick biology. Salivary miRNA datasets were re-analysed to identify conserved miRNAs, and putative target genes were predicted using the sialotranscriptomes of both species. In silico predictions were validated through experimental inhibition of specific miRNAs using antagomirs. Knockdown of miR-375 and miR-1 significantly reduced blood intake, oviposition, and fertility, indicating their involvement in feeding and reproductive processes. Silencing miR-252b in O. moubata led to increased mortality, suggesting a critical role in survival. Notably, Metis1 was identified as a likely target of miR-252b, and its dysregulation may underlie the observed lethality in miR-252b-silenced ticks. These findings highlight the functional relevance of salivary miRNAs in tick physiology and host interaction, offering new perspectives for the development of innovative tick control strategies.This work was supported by projects ‘RTI2018–098,297-B-I00’ and ‘PID2022–136644OB-I00’ (MCIU/AEI/FEDER, UE), granted by the Spanish Ministry of Science, Innovation and Universities, the State Research Agency (AEI), and the European Regional Development Fund (ERDF). In addition, Ana Laura Cano has been hired under the Generation D initiative, promoted by Red.es, an organization attached to the Ministry for Digital Transformation and the Civil Service, for the attraction and retention of talent through grants and training contracts, financed by the Recovery, Transformation and Resilience Plan through the European Union’s Next Generation funds (MMT24-IRNASA-01-01).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionMinisterio para la Transformación Digital y de la Función Pública (España)Cano Argüelles, Ana Laura [0000-0002-1056-8439]Pérez Sánchez, Ricardo [0000-0001-8875-439X]González-Sánchez, María [0000-0002-7176-3721]Oleaga, Ana [0000-0002-8019-7354]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/394159reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098297-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136644OB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/pathogens14060595Cano-Argüelles, A. L. (2025). Analysis of the tick-host interface and development of vaccines against Ornithodoros moubata. http://hdl.handle.net/10261/414435https://doi.org/10.3390/pathogens14060595Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3941592026-05-22T06:33:51Z
dc.title.none.fl_str_mv Insights into the Regulatory Roles of miRNAs in the Salivary Glands of the Soft Ticks Ornithodoros moubata and Ornithodoros erraticus
title Insights into the Regulatory Roles of miRNAs in the Salivary Glands of the Soft Ticks Ornithodoros moubata and Ornithodoros erraticus
spellingShingle Insights into the Regulatory Roles of miRNAs in the Salivary Glands of the Soft Ticks Ornithodoros moubata and Ornithodoros erraticus
Cano Argüelles, Ana Laura
Ornithodoros moubata
Ornithodoros erraticus
MicroRNA
Antagomir
Salivary glands
title_short Insights into the Regulatory Roles of miRNAs in the Salivary Glands of the Soft Ticks Ornithodoros moubata and Ornithodoros erraticus
title_full Insights into the Regulatory Roles of miRNAs in the Salivary Glands of the Soft Ticks Ornithodoros moubata and Ornithodoros erraticus
title_fullStr Insights into the Regulatory Roles of miRNAs in the Salivary Glands of the Soft Ticks Ornithodoros moubata and Ornithodoros erraticus
title_full_unstemmed Insights into the Regulatory Roles of miRNAs in the Salivary Glands of the Soft Ticks Ornithodoros moubata and Ornithodoros erraticus
title_sort Insights into the Regulatory Roles of miRNAs in the Salivary Glands of the Soft Ticks Ornithodoros moubata and Ornithodoros erraticus
dc.creator.none.fl_str_mv Cano Argüelles, Ana Laura
Pérez Sánchez, Ricardo
Gallardo Escárate, Cristian
Vizcaíno-Marín, Rocío
González-Sánchez, María
Oleaga, Ana
author Cano Argüelles, Ana Laura
author_facet Cano Argüelles, Ana Laura
Pérez Sánchez, Ricardo
Gallardo Escárate, Cristian
Vizcaíno-Marín, Rocío
González-Sánchez, María
Oleaga, Ana
author_role author
author2 Pérez Sánchez, Ricardo
Gallardo Escárate, Cristian
Vizcaíno-Marín, Rocío
González-Sánchez, María
Oleaga, Ana
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Ministerio para la Transformación Digital y de la Función Pública (España)
Cano Argüelles, Ana Laura [0000-0002-1056-8439]
Pérez Sánchez, Ricardo [0000-0001-8875-439X]
González-Sánchez, María [0000-0002-7176-3721]
Oleaga, Ana [0000-0002-8019-7354]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Ornithodoros moubata
Ornithodoros erraticus
MicroRNA
Antagomir
Salivary glands
topic Ornithodoros moubata
Ornithodoros erraticus
MicroRNA
Antagomir
Salivary glands
description MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by inhibiting or degrading messenger RNAs (mRNAs). In ticks, salivary miRNAs are proposed to play key roles in modulating host–vector interactions during blood feeding. Previously, we identified salivary miRNAs in Ornithodoros moubata and Ornithodoros erraticus, major vectors of African swine fever and tick-borne human relapsing fever. In this study, we investigated the regulatory roles of salivary miRNAs in tick biology. Salivary miRNA datasets were re-analysed to identify conserved miRNAs, and putative target genes were predicted using the sialotranscriptomes of both species. In silico predictions were validated through experimental inhibition of specific miRNAs using antagomirs. Knockdown of miR-375 and miR-1 significantly reduced blood intake, oviposition, and fertility, indicating their involvement in feeding and reproductive processes. Silencing miR-252b in O. moubata led to increased mortality, suggesting a critical role in survival. Notably, Metis1 was identified as a likely target of miR-252b, and its dysregulation may underlie the observed lethality in miR-252b-silenced ticks. These findings highlight the functional relevance of salivary miRNAs in tick physiology and host interaction, offering new perspectives for the development of innovative tick control strategies.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/394159
url http://hdl.handle.net/10261/394159
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098297-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136644OB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/pathogens14060595
Cano-Argüelles, A. L. (2025). Analysis of the tick-host interface and development of vaccines against Ornithodoros moubata. http://hdl.handle.net/10261/414435
https://doi.org/10.3390/pathogens14060595
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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