Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas

Lactococcus garvieae is a significant pathogen in aquaculture with a potential zoonotic risk. To begin to characterize the late immune response of trout to lactococcosis, we selected infected individuals showing clinical signs of lactococcosis. At the time lactococcosis clinical signs appeared, infe...

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Detalhes bibliográficos
Autores: Castro, Rosario, Coll, Julio, Blanco Gutiérrez, María Del Mar, Rodríguez Bertos, Antonio Manuel, Jouneau, Luc, Fernández-Garayzábal Fernández, José Francisco, Gibello Prieto, Alicia
Formato: artículo
Fecha de publicación:2019
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/93517
Acesso em linha:https://hdl.handle.net/20.500.14352/93517
Access Level:acceso abierto
Palavra-chave:636.09
Lactococcus garvieae
Gene expression
Spleen
Kidney
Trout infection
Veterinaria
31 Ciencias Agrarias
3109 Ciencias Veterinarias
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oai_identifier_str oai:docta.ucm.es:20.500.14352/93517
network_acronym_str ES
network_name_str España
repository_id_str
spelling Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomasCastro, RosarioColl, JulioBlanco Gutiérrez, María Del MarRodríguez Bertos, Antonio ManuelJouneau, LucFernández-Garayzábal Fernández, José FranciscoGibello Prieto, Alicia636.09Lactococcus garvieaeGene expressionSpleenKidneyTrout infectionVeterinaria31 Ciencias Agrarias3109 Ciencias VeterinariasLactococcus garvieae is a significant pathogen in aquaculture with a potential zoonotic risk. To begin to characterize the late immune response of trout to lactococcosis, we selected infected individuals showing clinical signs of lactococcosis. At the time lactococcosis clinical signs appeared, infection by L. garvieae induced a robust inflammatory response in the spleen of rainbow trout, which correlated with abundant granulomatous lesions. The response in kidney goes in parallel with that of spleen, and most of the gene regulations are similar in both organs. A correlation existed between the early inflammatory granulomas in spleen (containing macrophages with internalized L. garvieae) and up-regulated gene sets, which defined the presence of macrophages and neutrophils. This is the first analysis of the immune transcriptome of rainbow trout following L. garvieae infection during the initiation of adaptive immune mechanisms and shows a ranscriptome induction of antibody response by both IgM (+) and IgT (+) spleen B cells to respond to systemic infection. These results increase our understanding of lactococcosis and pave the way for future research to improve control measures of lactococcosis on fish farms.Spring NatureUniversidad Complutense de Madrid20192019-01-0120192019-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/93517reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/935172026-06-02T12:44:21Z
dc.title.none.fl_str_mv Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas
title Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas
spellingShingle Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas
Castro, Rosario
636.09
Lactococcus garvieae
Gene expression
Spleen
Kidney
Trout infection
Veterinaria
31 Ciencias Agrarias
3109 Ciencias Veterinarias
title_short Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas
title_full Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas
title_fullStr Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas
title_full_unstemmed Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas
title_sort Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas
dc.creator.none.fl_str_mv Castro, Rosario
Coll, Julio
Blanco Gutiérrez, María Del Mar
Rodríguez Bertos, Antonio Manuel
Jouneau, Luc
Fernández-Garayzábal Fernández, José Francisco
Gibello Prieto, Alicia
author Castro, Rosario
author_facet Castro, Rosario
Coll, Julio
Blanco Gutiérrez, María Del Mar
Rodríguez Bertos, Antonio Manuel
Jouneau, Luc
Fernández-Garayzábal Fernández, José Francisco
Gibello Prieto, Alicia
author_role author
author2 Coll, Julio
Blanco Gutiérrez, María Del Mar
Rodríguez Bertos, Antonio Manuel
Jouneau, Luc
Fernández-Garayzábal Fernández, José Francisco
Gibello Prieto, Alicia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.09
Lactococcus garvieae
Gene expression
Spleen
Kidney
Trout infection
Veterinaria
31 Ciencias Agrarias
3109 Ciencias Veterinarias
topic 636.09
Lactococcus garvieae
Gene expression
Spleen
Kidney
Trout infection
Veterinaria
31 Ciencias Agrarias
3109 Ciencias Veterinarias
description Lactococcus garvieae is a significant pathogen in aquaculture with a potential zoonotic risk. To begin to characterize the late immune response of trout to lactococcosis, we selected infected individuals showing clinical signs of lactococcosis. At the time lactococcosis clinical signs appeared, infection by L. garvieae induced a robust inflammatory response in the spleen of rainbow trout, which correlated with abundant granulomatous lesions. The response in kidney goes in parallel with that of spleen, and most of the gene regulations are similar in both organs. A correlation existed between the early inflammatory granulomas in spleen (containing macrophages with internalized L. garvieae) and up-regulated gene sets, which defined the presence of macrophages and neutrophils. This is the first analysis of the immune transcriptome of rainbow trout following L. garvieae infection during the initiation of adaptive immune mechanisms and shows a ranscriptome induction of antibody response by both IgM (+) and IgT (+) spleen B cells to respond to systemic infection. These results increase our understanding of lactococcosis and pave the way for future research to improve control measures of lactococcosis on fish farms.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/93517
url https://hdl.handle.net/20.500.14352/93517
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Spring Nature
publisher.none.fl_str_mv Spring Nature
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869417249126744064
score 15,81155

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