Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities

We report application of the knife-edge technique at the sharp edges of WS2 and MoS2 monolayer flakes for lateral and axial resolution assessment in all three modalities of nonlinear laser scanning microscopy: two-photon excited fluorescence (TPEF), second- and third-harmonic generation (SHG, THG) i...

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Autores: Jelić, Jovana Z., Bukumira, Marta, Denčevski, Aleksa, Senkić, Ana, Žužić, Livio, Radatovic, Borna, Vujičić, Nataša, Pajić, Tanja, Rabasović, Mihailo D., Krmpot, Aleksandar J.
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/394219
Acesso em linha:http://hdl.handle.net/10261/394219
https://api.elsevier.com/content/abstract/scopus_id/85202036221
Access Level:Acceso aberto
Palavra-chave:knife-edge technique
nonlinear laser scanning microscopy
resolution measurement
second/third harmonic generation
WS2 and MoS2 monolayers
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spelling Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy ModalitiesJelić, Jovana Z.Bukumira, MartaDenčevski, AleksaSenkić, AnaŽužić, LivioRadatovic, BornaVujičić, NatašaPajić, TanjaRabasović, Mihailo D.Krmpot, Aleksandar J.knife-edge techniquenonlinear laser scanning microscopyresolution measurementsecond/third harmonic generationWS2 and MoS2 monolayersWe report application of the knife-edge technique at the sharp edges of WS2 and MoS2 monolayer flakes for lateral and axial resolution assessment in all three modalities of nonlinear laser scanning microscopy: two-photon excited fluorescence (TPEF), second- and third-harmonic generation (SHG, THG) imaging. This technique provides a high signal-to-noise ratio, no photobleaching effect and shows good agreement with standard resolution measurement techniques. Furthermore, we assessed both the lateral resolution in TPEF imaging modality and the axial resolution in SHG and THG imaging modality directly via the full-width at half maximum parameter of the corresponding Gaussian distribution. We comprehensively analyzed the factors influencing the resolution, such as the numerical aperture, the excitation wavelength and the refractive index of the embedding medium for the different imaging modalities. Glycerin was identified as the optimal embedding medium for achieving resolutions closest to the theoretical limit. The proposed use of WS2 and MoS2 monolayer flakes emerged as promising tools for characterization of nonlinear imaging systems.This research was supported by the Science Fund of the Republic of Serbia, Grant No. 4545, project "Advanced Biophysical Methods for Soil Targeted Fungi-Based Biocontrol Agents" - BioPhysFUN; and the Institute of Physics Belgrade, through the grant by the Ministry of Science, Technological Development and Innovations of the Republic of Serbia. A.S., B.R., and N.V. acknowledge the support of Croatian Science Foundation, Grant No. UIP-2017-05-3869, Centre of Excellence for Advanced Materials and Sensing Devices, ERDF Grant No. KK.01.1.1.01.0001 and Centre for Advanced Laser Techniques, ERDF Grant KK.01.1.1.05.Peer reviewedOxford University Press0000-0003-3440-94640000-0003-2313-73440000-0002-2135-09100000-0002-0567-52990009-0002-6981-87000000-0001-5012-60050000-0002-5437-57860000-0003-3794-76550000-0002-9235-43880000-0003-2751-7395Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/394219https://api.elsevier.com/content/abstract/scopus_id/85202036221reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésMicroscopy and Microanalysishttps://doi.org/10.1093/mam/ozae061Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3942192026-05-22T06:33:51Z
dc.title.none.fl_str_mv Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities
title Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities
spellingShingle Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities
Jelić, Jovana Z.
knife-edge technique
nonlinear laser scanning microscopy
resolution measurement
second/third harmonic generation
WS2 and MoS2 monolayers
title_short Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities
title_full Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities
title_fullStr Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities
title_full_unstemmed Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities
title_sort Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities
dc.creator.none.fl_str_mv Jelić, Jovana Z.
Bukumira, Marta
Denčevski, Aleksa
Senkić, Ana
Žužić, Livio
Radatovic, Borna
Vujičić, Nataša
Pajić, Tanja
Rabasović, Mihailo D.
Krmpot, Aleksandar J.
author Jelić, Jovana Z.
author_facet Jelić, Jovana Z.
Bukumira, Marta
Denčevski, Aleksa
Senkić, Ana
Žužić, Livio
Radatovic, Borna
Vujičić, Nataša
Pajić, Tanja
Rabasović, Mihailo D.
Krmpot, Aleksandar J.
author_role author
author2 Bukumira, Marta
Denčevski, Aleksa
Senkić, Ana
Žužić, Livio
Radatovic, Borna
Vujičić, Nataša
Pajić, Tanja
Rabasović, Mihailo D.
Krmpot, Aleksandar J.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv 0000-0003-3440-9464
0000-0003-2313-7344
0000-0002-2135-0910
0000-0002-0567-5299
0009-0002-6981-8700
0000-0001-5012-6005
0000-0002-5437-5786
0000-0003-3794-7655
0000-0002-9235-4388
0000-0003-2751-7395
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv knife-edge technique
nonlinear laser scanning microscopy
resolution measurement
second/third harmonic generation
WS2 and MoS2 monolayers
topic knife-edge technique
nonlinear laser scanning microscopy
resolution measurement
second/third harmonic generation
WS2 and MoS2 monolayers
description We report application of the knife-edge technique at the sharp edges of WS2 and MoS2 monolayer flakes for lateral and axial resolution assessment in all three modalities of nonlinear laser scanning microscopy: two-photon excited fluorescence (TPEF), second- and third-harmonic generation (SHG, THG) imaging. This technique provides a high signal-to-noise ratio, no photobleaching effect and shows good agreement with standard resolution measurement techniques. Furthermore, we assessed both the lateral resolution in TPEF imaging modality and the axial resolution in SHG and THG imaging modality directly via the full-width at half maximum parameter of the corresponding Gaussian distribution. We comprehensively analyzed the factors influencing the resolution, such as the numerical aperture, the excitation wavelength and the refractive index of the embedding medium for the different imaging modalities. Glycerin was identified as the optimal embedding medium for achieving resolutions closest to the theoretical limit. The proposed use of WS2 and MoS2 monolayer flakes emerged as promising tools for characterization of nonlinear imaging systems.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/394219
https://api.elsevier.com/content/abstract/scopus_id/85202036221
url http://hdl.handle.net/10261/394219
https://api.elsevier.com/content/abstract/scopus_id/85202036221
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Microscopy and Microanalysis
https://doi.org/10.1093/mam/ozae061
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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