Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination

10 páginas, 6 figuras.

Detalles Bibliográficos
Autores: Morales, Sonia, Jiménez-López, José Carlos, Castro López, Antonio Jesús, Rodríguez García, María I., Alché Ramírez, Juan de Dios
Tipo de recurso: artículo
Fecha de publicación:2008
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/32758
Acceso en línea:http://hdl.handle.net/10261/32758
Access Level:acceso abierto
Palabra clave:Actin
Allergens
Cytoskeleton
Ole e 2
Olive trees
Pollen tube
Profilin
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spelling Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germinationMorales, SoniaJiménez-López, José CarlosCastro López, Antonio JesúsRodríguez García, María I.Alché Ramírez, Juan de DiosActinAllergensCytoskeletonOle e 2Olive treesPollen tubeProfilin10 páginas, 6 figuras.Pollen allergens offer a dual perspective of study: some of them are considered key proteins for pollen physiology, but they are also able to trigger allergy symptoms in susceptible humans after coming in contact with their tissues. Profilin (Ole e 2 allergen) has been characterized, to some extent, as one of the major allergens from Olea europaea L. pollen, a highly allergenic species in the Mediterranean countries. In order to obtain clues regarding the biological role of this protein, we have analyzed both its cellular localization and the organization of actin throughout pollen hydration and early pollen tube germination. The localization of the cited proteins was visualized by confocal laser scanning microscopy immunofluorescence using different antibodies. Upon pollen hydration and pollen germination, a massive presence of profilin was detected close to the site of pollen tube emergence, forming a ring-like structure around the ‘effective’ apertural region. Profilin was also detected in the pollen exine of the germinating pollen grains and in the germination medium. After using a permeabilization-enhanced protocol for immunolocalization, profilin was also localized in the cytoplasm of the pollen tube, particularly at both the proximal and apical ends. Noticeable accumulations of actin were observed in the cytoplasm of the pollen tube; particularly, in both the apical region and the area immediately close to the aperture. Actin filaments were not observed, probably due to the need of further enhanced fixation procedures. The ultrastructural localization of profilin showed the presence of the protein in the cytoplasm of both the mature pollen grain and the pollen tube. The results shown here could be interpreted as signs of a massive dissociation of the actin–profilin complexes, mobilization of actin monomers, and therefore, an intense activity of the actin cytoskeleton. The extensive release of allergenic proteins from the pollen grain into the surrounding aqueous media, as described here for profilin, may help us to understand the mechanisms by which these allergens might come in contact with the human mucosa, therefore triggering the symptoms of allergy.This work was funded by the Spanish Ministry for Education and Research (MEC) project BFU2004-00601/BFI and by the Consejería de Innovación, Ciencia y Empresa, Junta de Andalucia (JA), project P06-AGR-01791.Peer reviewedWiley-Blackwell201120112008info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/32758reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1111/j.1365-2818.2008.02044.xinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/327582026-05-22T06:33:51Z
dc.title.none.fl_str_mv Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination
title Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination
spellingShingle Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination
Morales, Sonia
Actin
Allergens
Cytoskeleton
Ole e 2
Olive trees
Pollen tube
Profilin
title_short Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination
title_full Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination
title_fullStr Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination
title_full_unstemmed Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination
title_sort Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination
dc.creator.none.fl_str_mv Morales, Sonia
Jiménez-López, José Carlos
Castro López, Antonio Jesús
Rodríguez García, María I.
Alché Ramírez, Juan de Dios
author Morales, Sonia
author_facet Morales, Sonia
Jiménez-López, José Carlos
Castro López, Antonio Jesús
Rodríguez García, María I.
Alché Ramírez, Juan de Dios
author_role author
author2 Jiménez-López, José Carlos
Castro López, Antonio Jesús
Rodríguez García, María I.
Alché Ramírez, Juan de Dios
author2_role author
author
author
author
dc.subject.none.fl_str_mv Actin
Allergens
Cytoskeleton
Ole e 2
Olive trees
Pollen tube
Profilin
topic Actin
Allergens
Cytoskeleton
Ole e 2
Olive trees
Pollen tube
Profilin
description 10 páginas, 6 figuras.
publishDate 2008
dc.date.none.fl_str_mv 2008
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/32758
url http://hdl.handle.net/10261/32758
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1111/j.1365-2818.2008.02044.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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