RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)

Purpose: In the framework of the ‘Realizing the European Network of Biodosimetry’ (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network pe...

Descripción completa

Detalles Bibliográficos
Autores: Depuydt, Julie, Baeyens, Ans, Barnard, Stephen, Beinke, Christina, Benedek, Anett, Beukes, Philip, Domínguez García, Inmaculada, Pastor Carrillo, Nuria María, Vral, Anne
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/130709
Acceso en línea:https://hdl.handle.net/11441/130709
https://doi.org/10.1080/09553002.2016.1206231
Access Level:acceso abierto
Palabra clave:Biodosimetry
micronucleus assay
triage
radiation accident
RENEB
id ES_b3fb3d8a2c9c8f387c9cce0ec6472cbf
oai_identifier_str oai:idus.us.es:11441/130709
network_acronym_str ES
network_name_str España
repository_id_str
spelling RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)Depuydt, JulieBaeyens, AnsBarnard, StephenBeinke, ChristinaBenedek, AnettBeukes, PhilipDomínguez García, InmaculadaPastor Carrillo, Nuria MaríaVral, AnneBiodosimetrymicronucleus assaytriageradiation accidentRENEBPurpose: In the framework of the ‘Realizing the European Network of Biodosimetry’ (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one. Materials and methods: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate. Micronucleus analysis was performed by automated, semi-automated and manual procedures. Results: The dose estimates provided by network laboratories were in good agreement with true administered doses. The most accurate estimates were reported for low dose points (≤ 0.94 Gy). For higher dose points (≥ 2.7 Gy) a larger variation in estimates was observed, though in the second exercise the number of acceptable estimates increased satisfactorily. Higher accuracy was achieved with the semi-automated method. Conclusion: The results of the two exercises performed by our network demonstrate that the micronucleus assay is a useful tool for large-scale radiation emergencies, and can be successfully implemented within a large network of laboratories.VII Programa Marco de Investigación y Desarrollo (VIIPM) de la Unión Europea. nº 295513University Development Cooperation "VLIR Own Initiative Programme" enetre Bélgica and Sudáfrica. ZEIN2011PR387Taylor & Francis LTDBiología CelularEuropean Union2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/130709https://doi.org/10.1080/09553002.2016.1206231reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésInternational Journal of Radiation Biology, 93 (1), 36-47.VIIPM 295513ZEIN2011PR387https://doi.org/10.1080/09553002.2016.1206231info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1307092026-06-17T12:51:07Z
dc.title.none.fl_str_mv RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)
title RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)
spellingShingle RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)
Depuydt, Julie
Biodosimetry
micronucleus assay
triage
radiation accident
RENEB
title_short RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)
title_full RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)
title_fullStr RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)
title_full_unstemmed RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)
title_sort RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)
dc.creator.none.fl_str_mv Depuydt, Julie
Baeyens, Ans
Barnard, Stephen
Beinke, Christina
Benedek, Anett
Beukes, Philip
Domínguez García, Inmaculada
Pastor Carrillo, Nuria María
Vral, Anne
author Depuydt, Julie
author_facet Depuydt, Julie
Baeyens, Ans
Barnard, Stephen
Beinke, Christina
Benedek, Anett
Beukes, Philip
Domínguez García, Inmaculada
Pastor Carrillo, Nuria María
Vral, Anne
author_role author
author2 Baeyens, Ans
Barnard, Stephen
Beinke, Christina
Benedek, Anett
Beukes, Philip
Domínguez García, Inmaculada
Pastor Carrillo, Nuria María
Vral, Anne
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biología Celular
European Union
dc.subject.none.fl_str_mv Biodosimetry
micronucleus assay
triage
radiation accident
RENEB
topic Biodosimetry
micronucleus assay
triage
radiation accident
RENEB
description Purpose: In the framework of the ‘Realizing the European Network of Biodosimetry’ (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one. Materials and methods: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate. Micronucleus analysis was performed by automated, semi-automated and manual procedures. Results: The dose estimates provided by network laboratories were in good agreement with true administered doses. The most accurate estimates were reported for low dose points (≤ 0.94 Gy). For higher dose points (≥ 2.7 Gy) a larger variation in estimates was observed, though in the second exercise the number of acceptable estimates increased satisfactorily. Higher accuracy was achieved with the semi-automated method. Conclusion: The results of the two exercises performed by our network demonstrate that the micronucleus assay is a useful tool for large-scale radiation emergencies, and can be successfully implemented within a large network of laboratories.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/130709
https://doi.org/10.1080/09553002.2016.1206231
url https://hdl.handle.net/11441/130709
https://doi.org/10.1080/09553002.2016.1206231
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Radiation Biology, 93 (1), 36-47.
VIIPM 295513
ZEIN2011PR387
https://doi.org/10.1080/09553002.2016.1206231
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis LTD
publisher.none.fl_str_mv Taylor & Francis LTD
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869417223188119552
score 15,300719

Similares en LA Referencia