Inflammatory Gene Expression In Whole Peripheral Blood At Early Stage Of Sporadic Amyotrophic Lateral Sclerosis

Objective: Characterization of altered expression of selected transcripts linked to inflammation in the peripheral blood of sporadic amyotrophic lateral sclerosis (sALS) patients at early stage of disease to increase knowledge about peripheral inflammatory response in sALS. Methods: RNA expression l...

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Autores: Andrés Benito, Pol, Moreno Castro, Jesús, Domínguez, Raúl, Aso Pérez, Ester, Povedano, Mònica, Ferrer, Isidro (Ferrer Abizanda)
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/124240
Acceso en línea:https://hdl.handle.net/2445/124240
Access Level:acceso abierto
Palabra clave:Esclerosi lateral amiotròfica
Citoquines
Amyotrophic lateral sclerosis
Cytokines
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spelling Inflammatory Gene Expression In Whole Peripheral Blood At Early Stage Of Sporadic Amyotrophic Lateral SclerosisAndrés Benito, PolMoreno Castro, JesúsDomínguez, RaúlAso Pérez, EsterPovedano, MònicaFerrer, Isidro (Ferrer Abizanda)Esclerosi lateral amiotròficaCitoquinesAmyotrophic lateral sclerosisCytokinesObjective: Characterization of altered expression of selected transcripts linked to inflammation in the peripheral blood of sporadic amyotrophic lateral sclerosis (sALS) patients at early stage of disease to increase knowledge about peripheral inflammatory response in sALS. Methods: RNA expression levels of 45 genes were assessed by RT-qPCR in 22 sALS cases in parallel with 13 age-matched controls. Clinical and serum parameters were assessed at the same time. Results: Upregulation of genes coding for factors involved in leukocyte extravasation (ITGB2, INPP5D, SELL, and ICAM1) and extracellular matrix remodeling (MMP9 and TIMP2), as well as downregulation of certain chemokines (CCL5 and CXC5R), antiinflammatory cytokines (IL10, TGFB2, and IL10RA), pro-inflammatory cytokines (IL-6), and T-cell regulators (CD2 and TRBC1) was found in sALS cases independently of gender, clinical symptoms at onset (spinal, respiratory, or bulbar), progression, peripheral leukocyte number, and integrity of RNA. MMP9 levels positively correlated with age, whereas CCR5, CCL5, and TRBC1 negatively correlated with age in sALS but not in controls. Relatively higher TNFA expression levels correlate with higher creatinine kinase protein levels in plasma. Conclusion: Present findings show early inflammatory responses characterized by upregulation of factors enabling extravasation of leukocytes and extracellular matrix remodeling in blood in sALS cases, in addition to increased TNFA levels paralleling skeletal muscle damage.Frontiers Media2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/124240Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fneur.2017.00546Frontiers in Neurology, 2017, vol. 8https://doi.org/10.3389/fneur.2017.00546cc by (c) Andrés Benito et al., 2017http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1242402026-05-27T06:46:51Z
dc.title.none.fl_str_mv Inflammatory Gene Expression In Whole Peripheral Blood At Early Stage Of Sporadic Amyotrophic Lateral Sclerosis
title Inflammatory Gene Expression In Whole Peripheral Blood At Early Stage Of Sporadic Amyotrophic Lateral Sclerosis
spellingShingle Inflammatory Gene Expression In Whole Peripheral Blood At Early Stage Of Sporadic Amyotrophic Lateral Sclerosis
Andrés Benito, Pol
Esclerosi lateral amiotròfica
Citoquines
Amyotrophic lateral sclerosis
Cytokines
title_short Inflammatory Gene Expression In Whole Peripheral Blood At Early Stage Of Sporadic Amyotrophic Lateral Sclerosis
title_full Inflammatory Gene Expression In Whole Peripheral Blood At Early Stage Of Sporadic Amyotrophic Lateral Sclerosis
title_fullStr Inflammatory Gene Expression In Whole Peripheral Blood At Early Stage Of Sporadic Amyotrophic Lateral Sclerosis
title_full_unstemmed Inflammatory Gene Expression In Whole Peripheral Blood At Early Stage Of Sporadic Amyotrophic Lateral Sclerosis
title_sort Inflammatory Gene Expression In Whole Peripheral Blood At Early Stage Of Sporadic Amyotrophic Lateral Sclerosis
dc.creator.none.fl_str_mv Andrés Benito, Pol
Moreno Castro, Jesús
Domínguez, Raúl
Aso Pérez, Ester
Povedano, Mònica
Ferrer, Isidro (Ferrer Abizanda)
author Andrés Benito, Pol
author_facet Andrés Benito, Pol
Moreno Castro, Jesús
Domínguez, Raúl
Aso Pérez, Ester
Povedano, Mònica
Ferrer, Isidro (Ferrer Abizanda)
author_role author
author2 Moreno Castro, Jesús
Domínguez, Raúl
Aso Pérez, Ester
Povedano, Mònica
Ferrer, Isidro (Ferrer Abizanda)
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Esclerosi lateral amiotròfica
Citoquines
Amyotrophic lateral sclerosis
Cytokines
topic Esclerosi lateral amiotròfica
Citoquines
Amyotrophic lateral sclerosis
Cytokines
description Objective: Characterization of altered expression of selected transcripts linked to inflammation in the peripheral blood of sporadic amyotrophic lateral sclerosis (sALS) patients at early stage of disease to increase knowledge about peripheral inflammatory response in sALS. Methods: RNA expression levels of 45 genes were assessed by RT-qPCR in 22 sALS cases in parallel with 13 age-matched controls. Clinical and serum parameters were assessed at the same time. Results: Upregulation of genes coding for factors involved in leukocyte extravasation (ITGB2, INPP5D, SELL, and ICAM1) and extracellular matrix remodeling (MMP9 and TIMP2), as well as downregulation of certain chemokines (CCL5 and CXC5R), antiinflammatory cytokines (IL10, TGFB2, and IL10RA), pro-inflammatory cytokines (IL-6), and T-cell regulators (CD2 and TRBC1) was found in sALS cases independently of gender, clinical symptoms at onset (spinal, respiratory, or bulbar), progression, peripheral leukocyte number, and integrity of RNA. MMP9 levels positively correlated with age, whereas CCR5, CCL5, and TRBC1 negatively correlated with age in sALS but not in controls. Relatively higher TNFA expression levels correlate with higher creatinine kinase protein levels in plasma. Conclusion: Present findings show early inflammatory responses characterized by upregulation of factors enabling extravasation of leukocytes and extracellular matrix remodeling in blood in sALS cases, in addition to increased TNFA levels paralleling skeletal muscle damage.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/124240
url https://hdl.handle.net/2445/124240
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fneur.2017.00546
Frontiers in Neurology, 2017, vol. 8
https://doi.org/10.3389/fneur.2017.00546
dc.rights.none.fl_str_mv cc by (c) Andrés Benito et al., 2017
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Andrés Benito et al., 2017
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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